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Proteome Analysis and In Vitro Antiviral, Anticancer and Antioxidant Capacities of the Aqueous Extracts of Lentinula edodes and Pleurotus ostreatus Edible Mushrooms.
Molecules. 2021 Jul 30; 26(15)M

Abstract

In this study, we examined aqueous extracts of the edible mushrooms Pleurotus ostreatus (oyster mushroom) and Lentinula edodes (shiitake mushroom). Proteome analysis was conducted using LC-Triple TOF-MS and showed the expression of 753 proteins by Pleurotus ostreatus, and 432 proteins by Lentinula edodes. Bioactive peptides: Rab GDP dissociation inhibitor, superoxide dismutase, thioredoxin reductase, serine proteinase and lectin, were identified in both mushrooms. The extracts also included promising bioactive compounds including phenolics, flavonoids, vitamins and amino acids. The extracts showed promising antiviral activities, with a selectivity index (SI) of 4.5 for Pleurotus ostreatus against adenovirus (Ad7), and a slight activity for Lentinula edodes against herpes simplex-II (HSV-2). The extracts were not cytotoxic to normal human peripheral blood mononuclear cells (PBMCs). On the contrary, they showed moderate cytotoxicity against various cancer cell lines. Additionally, antioxidant activity was assessed using DPPH radical scavenging, ABTS radical cation scavenging and ORAC assays. The two extracts showed potential antioxidant activities, with the maximum activity seen for Pleurotus ostreatus (IC50 µg/mL) = 39.46 ± 1.27 for DPPH; 11.22 ± 1.81 for ABTS; and 21.40 ± 2.20 for ORAC assays. This study encourages the use of these mushrooms in medicine in the light of their low cytotoxicity on normal PBMCs vis à vis their antiviral, antitumor and antioxidant capabilities.

Authors+Show Affiliations

Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University (ACU), 4th Industrial Area, 6th of October City, Cairo 2566, Egypt.Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University (ACU), 4th Industrial Area, 6th of October City, Cairo 2566, Egypt. Department of Microbiology and Immunology, Faculty of Pharmacy, Helwan University, Cairo 11795, Egypt.Department of Biology, College of Science, Taif University, Taif 11099, Saudi Arabia.Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Cairo 11566, Egypt.Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Cairo 11566, Egypt.Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Cairo 11566, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34361776

Citation

Elhusseiny, Shaza M., et al. "Proteome Analysis and in Vitro Antiviral, Anticancer and Antioxidant Capacities of the Aqueous Extracts of Lentinula Edodes and Pleurotus Ostreatus Edible Mushrooms." Molecules (Basel, Switzerland), vol. 26, no. 15, 2021.
Elhusseiny SM, El-Mahdy TS, Awad MF, et al. Proteome Analysis and In Vitro Antiviral, Anticancer and Antioxidant Capacities of the Aqueous Extracts of Lentinula edodes and Pleurotus ostreatus Edible Mushrooms. Molecules. 2021;26(15).
Elhusseiny, S. M., El-Mahdy, T. S., Awad, M. F., Elleboudy, N. S., Farag, M. M. S., Yassein, M. A., & Aboshanab, K. M. (2021). Proteome Analysis and In Vitro Antiviral, Anticancer and Antioxidant Capacities of the Aqueous Extracts of Lentinula edodes and Pleurotus ostreatus Edible Mushrooms. Molecules (Basel, Switzerland), 26(15). https://doi.org/10.3390/molecules26154623
Elhusseiny SM, et al. Proteome Analysis and in Vitro Antiviral, Anticancer and Antioxidant Capacities of the Aqueous Extracts of Lentinula Edodes and Pleurotus Ostreatus Edible Mushrooms. Molecules. 2021 Jul 30;26(15) PubMed PMID: 34361776.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteome Analysis and In Vitro Antiviral, Anticancer and Antioxidant Capacities of the Aqueous Extracts of Lentinula edodes and Pleurotus ostreatus Edible Mushrooms. AU - Elhusseiny,Shaza M, AU - El-Mahdy,Taghrid S, AU - Awad,Mohamed F, AU - Elleboudy,Nooran S, AU - Farag,Mohamed M S, AU - Yassein,Mahmoud A, AU - Aboshanab,Khaled M, Y1 - 2021/07/30/ PY - 2021/06/30/received PY - 2021/07/22/revised PY - 2021/07/28/accepted PY - 2021/8/7/entrez PY - 2021/8/8/pubmed PY - 2021/9/18/medline KW - Lentinula edodes KW - Pleurotus ostreatus KW - antioxidant KW - antitumor KW - antiviral KW - shiitake KW - white rot fungus JF - Molecules (Basel, Switzerland) JO - Molecules VL - 26 IS - 15 N2 - In this study, we examined aqueous extracts of the edible mushrooms Pleurotus ostreatus (oyster mushroom) and Lentinula edodes (shiitake mushroom). Proteome analysis was conducted using LC-Triple TOF-MS and showed the expression of 753 proteins by Pleurotus ostreatus, and 432 proteins by Lentinula edodes. Bioactive peptides: Rab GDP dissociation inhibitor, superoxide dismutase, thioredoxin reductase, serine proteinase and lectin, were identified in both mushrooms. The extracts also included promising bioactive compounds including phenolics, flavonoids, vitamins and amino acids. The extracts showed promising antiviral activities, with a selectivity index (SI) of 4.5 for Pleurotus ostreatus against adenovirus (Ad7), and a slight activity for Lentinula edodes against herpes simplex-II (HSV-2). The extracts were not cytotoxic to normal human peripheral blood mononuclear cells (PBMCs). On the contrary, they showed moderate cytotoxicity against various cancer cell lines. Additionally, antioxidant activity was assessed using DPPH radical scavenging, ABTS radical cation scavenging and ORAC assays. The two extracts showed potential antioxidant activities, with the maximum activity seen for Pleurotus ostreatus (IC50 µg/mL) = 39.46 ± 1.27 for DPPH; 11.22 ± 1.81 for ABTS; and 21.40 ± 2.20 for ORAC assays. This study encourages the use of these mushrooms in medicine in the light of their low cytotoxicity on normal PBMCs vis à vis their antiviral, antitumor and antioxidant capabilities. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/34361776/Proteome_Analysis_and_In_Vitro_Antiviral_Anticancer_and_Antioxidant_Capacities_of_the_Aqueous_Extracts_of_Lentinula_edodes_and_Pleurotus_ostreatus_Edible_Mushrooms_ L2 - https://www.mdpi.com/resolver?pii=molecules26154623 DB - PRIME DP - Unbound Medicine ER -