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Abnormal regulation of microRNAs and related genes in pediatric β-thalassemia.
J Clin Lab Anal. 2021 Sep; 35(9):e23945.JC

Abstract

BACKGROUND

MicroRNAs (miRNAs) participate in the reactivation of γ-globin expression in β-thalassemia. However, the miRNA transcriptional profiles of pediatric β-thalassemia remain unclear. Accordingly, in this study, we assessed miRNA expression in pediatric patients with β-thalassemia.

METHODS

Differentially expressed miRNAs in pediatric patients with β-thalassemia were determined using microRNA sequencing.

RESULTS

Hsa-miR-483-3p, hsa-let-7f-1-3p, hsa-let-7a-3p, hsa-miR-543, hsa-miR-433-3p, hsa-miR-4435, hsa-miR-329-3p, hsa-miR-92b-5p, hsa-miR-6747-3p and hsa-miR-495-3p were significantly upregulated, whereas hsa-miR-4508, hsa-miR-20a-5p, hsa-let-7b-5p, hsa-miR-93-5p, hsa-let-7i-5p, hsa-miR-6501-5p, hsa-miR-221-3p, hsa-let-7g-5p, hsa-miR-106a-5p, and hsa-miR-17-5p were significantly downregulated in pediatric patients with β-thalassemia. After integrating our data with a previously published dataset, we found that hsa-let-7b-5p and hsa-let-7i-5p expression levels were also lower in adolescent or adult patients with β-thalassemia. The predicted target genes of hsa-let-7b-5p and hsa-let-7i-5p were associated with the transforming growth factor β receptor, phosphatidylinositol 3-kinase/AKT, FoxO, Hippo, and mitogen-activated protein kinase signaling pathways. We also identified 12 target genes of hsa-let-7a-3p and hsa-let-7f-1-3p and 21 target genes of hsa-let-7a-3p and hsa-let-7f-1-3p, which were differentially expressed in patients with β-thalassemia. Finally, we found that hsa-miR-190-5p and hsa-miR-1278-5p may regulate hemoglobin switching by modulation of the B-cell lymphoma/leukemia 11A gene.

CONCLUSION

The results of the study show that several microRNAs are dysregulated in pediatric β-thalassemia. Further, the results also indicate toward a critical role of let7 miRNAs in the pathogenesis of pediatric β-thalassemia, which needs to be investigated further.

Links

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Authors+Show Affiliations

Wang H
Medical Genetic Diagnosis and Therapy Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Chen M
Medical Genetic Diagnosis and Therapy Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Xu S
Guangxi Medical University, Nanning, China.
Pan Y
Medical Technology and Engineering College of Fujian Medical University, Fuzhou, China.
Zhang Y
Fujian University of Traditional Chinese Medicine, Fuzhou, China.
Huang H
Medical Genetic Diagnosis and Therapy Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Xu L
Medical Genetic Diagnosis and Therapy Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, China.

MeSH

Case-Control StudiesChild, PreschoolFemaleGene Expression ProfilingGene Expression RegulationGene Regulatory NetworksHumansMaleMicroRNAsPrognosisbeta-Thalassemia

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34398996

Citation

Wang, Haiwei, et al. "Abnormal Regulation of microRNAs and Related Genes in Pediatric Β-thalassemia." Journal of Clinical Laboratory Analysis, vol. 35, no. 9, 2021, pp. e23945.
Wang H, Chen M, Xu S, et al. Abnormal regulation of microRNAs and related genes in pediatric β-thalassemia. J Clin Lab Anal. 2021;35(9):e23945.
Wang, H., Chen, M., Xu, S., Pan, Y., Zhang, Y., Huang, H., & Xu, L. (2021). Abnormal regulation of microRNAs and related genes in pediatric β-thalassemia. Journal of Clinical Laboratory Analysis, 35(9), e23945. https://doi.org/10.1002/jcla.23945
Wang H, et al. Abnormal Regulation of microRNAs and Related Genes in Pediatric Β-thalassemia. J Clin Lab Anal. 2021;35(9):e23945. PubMed PMID: 34398996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Abnormal regulation of microRNAs and related genes in pediatric β-thalassemia. AU - Wang,Haiwei, AU - Chen,Meihuan, AU - Xu,Shiyi, AU - Pan,Yali, AU - Zhang,Yanhong, AU - Huang,Hailong, AU - Xu,Liangpu, Y1 - 2021/08/16/ PY - 2021/07/19/revised PY - 2021/05/06/received PY - 2021/07/27/accepted PY - 2021/8/17/pubmed PY - 2022/1/27/medline PY - 2021/8/16/entrez KW - B-cell lymphoma/leukemia 11A KW - let7 microRNA KW - microRNA sequencing KW - pediatric β-thalassemia KW - γ-globin reactivation SP - e23945 EP - e23945 JF - Journal of clinical laboratory analysis JO - J Clin Lab Anal VL - 35 IS - 9 N2 - BACKGROUND: MicroRNAs (miRNAs) participate in the reactivation of γ-globin expression in β-thalassemia. However, the miRNA transcriptional profiles of pediatric β-thalassemia remain unclear. Accordingly, in this study, we assessed miRNA expression in pediatric patients with β-thalassemia. METHODS: Differentially expressed miRNAs in pediatric patients with β-thalassemia were determined using microRNA sequencing. RESULTS: Hsa-miR-483-3p, hsa-let-7f-1-3p, hsa-let-7a-3p, hsa-miR-543, hsa-miR-433-3p, hsa-miR-4435, hsa-miR-329-3p, hsa-miR-92b-5p, hsa-miR-6747-3p and hsa-miR-495-3p were significantly upregulated, whereas hsa-miR-4508, hsa-miR-20a-5p, hsa-let-7b-5p, hsa-miR-93-5p, hsa-let-7i-5p, hsa-miR-6501-5p, hsa-miR-221-3p, hsa-let-7g-5p, hsa-miR-106a-5p, and hsa-miR-17-5p were significantly downregulated in pediatric patients with β-thalassemia. After integrating our data with a previously published dataset, we found that hsa-let-7b-5p and hsa-let-7i-5p expression levels were also lower in adolescent or adult patients with β-thalassemia. The predicted target genes of hsa-let-7b-5p and hsa-let-7i-5p were associated with the transforming growth factor β receptor, phosphatidylinositol 3-kinase/AKT, FoxO, Hippo, and mitogen-activated protein kinase signaling pathways. We also identified 12 target genes of hsa-let-7a-3p and hsa-let-7f-1-3p and 21 target genes of hsa-let-7a-3p and hsa-let-7f-1-3p, which were differentially expressed in patients with β-thalassemia. Finally, we found that hsa-miR-190-5p and hsa-miR-1278-5p may regulate hemoglobin switching by modulation of the B-cell lymphoma/leukemia 11A gene. CONCLUSION: The results of the study show that several microRNAs are dysregulated in pediatric β-thalassemia. Further, the results also indicate toward a critical role of let7 miRNAs in the pathogenesis of pediatric β-thalassemia, which needs to be investigated further. SN - 1098-2825 UR - https://www.unboundmedicine.com/medline/citation/34398996/Abnormal_regulation_of_microRNAs_and_related_genes_in_pediatric_β_thalassemia_ DB - PRIME DP - Unbound Medicine ER -