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Protective effects of crocin on testicular torsion/detorsion in rats.
Theriogenology. 2021 Oct 01; 173:241-248.T

Abstract

Oxidative stress, caused by extreme accumulation of un-scavenged reactive oxygen species, plays an integral role in the Ischemia-Reperfusion (I/R) injury to the testicles following testicular torsion. The current research aimed to examine the protective effects of crocin as a natural antioxidant on testicular I/R injury in rats. Animals were divided randomly into five groups (seven each): (1) sham group, (2) torsion/detorsion (T/D) group, (3) intact group with 100 mg/kg crocin, (4) and (5) T/D groups followed by treatment with two different doses of crocin (50 and 100 mg/kg (IP)). I/R injury was induced by 720° clockwise torsion of the left testicles for 2 h. After 24 h of reperfusion, blood samples and epididymal sperms were collected to measure biochemical (GPx, SOD, and MDA), hormonal (testosterone), and sperm parameters (total sperm recovery, motility, viability, and morphology). Moreover, affected testicles were subjected to histopathology examination. I/R injury caused a significant reduction in sperm characteristics (except for morphology) (P < 0.05), which could not be significantly improved by crocin administration at either dose (P > 0.05). Johnsen's testicular score, mean seminiferous tubular diameter, and germinal epithelial cell thickness were significantly decreased in the T/D group compared to the intact and sham groups. However, crocin could significantly improve the histopathological parameters in both treatment groups compared to the T/D group (P < 0.05). T/D reduced SOD and GPx activity and testosterone level significantly (except for GPx) compared to the sham group (P < 0.05). However, crocin administration could significantly reverse them. Also, crocin reduced the amount of MDA significantly in the high-dose treatment group in comparison to T/D group (P < 0.05). The results of the current study revealed that crocin could be a promising agent to protect against I/R injury following surgical correction of the testicular torsion.

Authors+Show Affiliations

Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. Electronic address: v.ganjiani@yahoo.com.Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. Electronic address: nahmadi@shirazu.ac.ir.Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. Electronic address: mrdivar@gmail.com.Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. Electronic address: sharifiy@shirazu.ac.ir.Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. Electronic address: meimandi@shirazu.ac.ir.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34399388

Citation

Ganjiani, Vahid, et al. "Protective Effects of Crocin On Testicular Torsion/detorsion in Rats." Theriogenology, vol. 173, 2021, pp. 241-248.
Ganjiani V, Ahmadi N, Divar MR, et al. Protective effects of crocin on testicular torsion/detorsion in rats. Theriogenology. 2021;173:241-248.
Ganjiani, V., Ahmadi, N., Divar, M. R., Sharifiyazdi, H., & Meimandi-Parizi, A. (2021). Protective effects of crocin on testicular torsion/detorsion in rats. Theriogenology, 173, 241-248. https://doi.org/10.1016/j.theriogenology.2021.07.021
Ganjiani V, et al. Protective Effects of Crocin On Testicular Torsion/detorsion in Rats. Theriogenology. 2021 Oct 1;173:241-248. PubMed PMID: 34399388.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of crocin on testicular torsion/detorsion in rats. AU - Ganjiani,Vahid, AU - Ahmadi,Nasrollah, AU - Divar,Mohammad Reza, AU - Sharifiyazdi,Hassan, AU - Meimandi-Parizi,Abdolhamid, Y1 - 2021/08/06/ PY - 2020/11/09/received PY - 2021/07/27/revised PY - 2021/07/31/accepted PY - 2021/8/17/pubmed PY - 2021/8/27/medline PY - 2021/8/16/entrez KW - Crocin KW - Ischemia/reperfusion injury KW - Testis SP - 241 EP - 248 JF - Theriogenology JO - Theriogenology VL - 173 N2 - Oxidative stress, caused by extreme accumulation of un-scavenged reactive oxygen species, plays an integral role in the Ischemia-Reperfusion (I/R) injury to the testicles following testicular torsion. The current research aimed to examine the protective effects of crocin as a natural antioxidant on testicular I/R injury in rats. Animals were divided randomly into five groups (seven each): (1) sham group, (2) torsion/detorsion (T/D) group, (3) intact group with 100 mg/kg crocin, (4) and (5) T/D groups followed by treatment with two different doses of crocin (50 and 100 mg/kg (IP)). I/R injury was induced by 720° clockwise torsion of the left testicles for 2 h. After 24 h of reperfusion, blood samples and epididymal sperms were collected to measure biochemical (GPx, SOD, and MDA), hormonal (testosterone), and sperm parameters (total sperm recovery, motility, viability, and morphology). Moreover, affected testicles were subjected to histopathology examination. I/R injury caused a significant reduction in sperm characteristics (except for morphology) (P < 0.05), which could not be significantly improved by crocin administration at either dose (P > 0.05). Johnsen's testicular score, mean seminiferous tubular diameter, and germinal epithelial cell thickness were significantly decreased in the T/D group compared to the intact and sham groups. However, crocin could significantly improve the histopathological parameters in both treatment groups compared to the T/D group (P < 0.05). T/D reduced SOD and GPx activity and testosterone level significantly (except for GPx) compared to the sham group (P < 0.05). However, crocin administration could significantly reverse them. Also, crocin reduced the amount of MDA significantly in the high-dose treatment group in comparison to T/D group (P < 0.05). The results of the current study revealed that crocin could be a promising agent to protect against I/R injury following surgical correction of the testicular torsion. SN - 1879-3231 UR - https://www.unboundmedicine.com/medline/citation/34399388/Protective_effects_of_crocin_on_testicular_torsion/detorsion_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0093-691X(21)00246-6 DB - PRIME DP - Unbound Medicine ER -