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TRPV1 and TRPA1 Channels Are Both Involved Downstream of Histamine-Induced Itch.
Biomolecules. 2021 08 06; 11(8)B

Abstract

Two histamine receptor subtypes (HR), namely H1R and H4R, are involved in the transmission of histamine-induced itch as key components. Although exact downstream signaling mechanisms are still elusive, transient receptor potential (TRP) ion channels play important roles in the sensation of histaminergic and non-histaminergic itch. The aim of this study was to investigate the involvement of TRPV1 and TRPA1 channels in the transmission of histaminergic itch. The potential of TRPV1 and TRPA1 inhibitors to modulate H1R- and H4R-induced signal transmission was tested in a scratching assay in mice in vivo as well as via Ca2+ imaging of murine sensory dorsal root ganglia (DRG) neurons in vitro. TRPV1 inhibition led to a reduction of H1R- and H4R- induced itch, whereas TRPA1 inhibition reduced H4R- but not H1R-induced itch. TRPV1 and TRPA1 inhibition resulted in a reduced Ca2+ influx into sensory neurons in vitro. In conclusion, these results indicate that both channels, TRPV1 and TRPA1, are involved in the transmission of histamine-induced pruritus.

Authors+Show Affiliations

Department of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, Germany. Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA. Department of Veterinary Medicine, Institute of Pharmacology and Toxicology, Freie Universität Berlin, 14195 Berlin, Germany.Department of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, Germany.Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA.Institute of Pharmaceutical and Medical Chemistry, Heinrich Heine University Düsseldorf, 40225 Duesseldorf, Germany.Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA. Department of Veterinary Medicine, Institute of Pharmacology and Toxicology, Freie Universität Berlin, 14195 Berlin, Germany.Department of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, Germany.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

34439832

Citation

Wilzopolski, Jenny, et al. "TRPV1 and TRPA1 Channels Are Both Involved Downstream of Histamine-Induced Itch." Biomolecules, vol. 11, no. 8, 2021.
Wilzopolski J, Kietzmann M, Mishra SK, et al. TRPV1 and TRPA1 Channels Are Both Involved Downstream of Histamine-Induced Itch. Biomolecules. 2021;11(8).
Wilzopolski, J., Kietzmann, M., Mishra, S. K., Stark, H., Bäumer, W., & Rossbach, K. (2021). TRPV1 and TRPA1 Channels Are Both Involved Downstream of Histamine-Induced Itch. Biomolecules, 11(8). https://doi.org/10.3390/biom11081166
Wilzopolski J, et al. TRPV1 and TRPA1 Channels Are Both Involved Downstream of Histamine-Induced Itch. Biomolecules. 2021 08 6;11(8) PubMed PMID: 34439832.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TRPV1 and TRPA1 Channels Are Both Involved Downstream of Histamine-Induced Itch. AU - Wilzopolski,Jenny, AU - Kietzmann,Manfred, AU - Mishra,Santosh K, AU - Stark,Holger, AU - Bäumer,Wolfgang, AU - Rossbach,Kristine, Y1 - 2021/08/06/ PY - 2021/07/13/received PY - 2021/07/31/revised PY - 2021/08/04/accepted PY - 2021/8/27/entrez PY - 2021/8/28/pubmed PY - 2021/9/28/medline KW - Ca2+-imaging KW - TRPA1 KW - TRPV1 KW - dorsal root ganglion neurons (DRG) KW - histamine KW - histamine H1 receptor KW - histamine H4 receptor KW - itch KW - signal transduction JF - Biomolecules JO - Biomolecules VL - 11 IS - 8 N2 - Two histamine receptor subtypes (HR), namely H1R and H4R, are involved in the transmission of histamine-induced itch as key components. Although exact downstream signaling mechanisms are still elusive, transient receptor potential (TRP) ion channels play important roles in the sensation of histaminergic and non-histaminergic itch. The aim of this study was to investigate the involvement of TRPV1 and TRPA1 channels in the transmission of histaminergic itch. The potential of TRPV1 and TRPA1 inhibitors to modulate H1R- and H4R-induced signal transmission was tested in a scratching assay in mice in vivo as well as via Ca2+ imaging of murine sensory dorsal root ganglia (DRG) neurons in vitro. TRPV1 inhibition led to a reduction of H1R- and H4R- induced itch, whereas TRPA1 inhibition reduced H4R- but not H1R-induced itch. TRPV1 and TRPA1 inhibition resulted in a reduced Ca2+ influx into sensory neurons in vitro. In conclusion, these results indicate that both channels, TRPV1 and TRPA1, are involved in the transmission of histamine-induced pruritus. SN - 2218-273X UR - https://www.unboundmedicine.com/medline/citation/34439832/TRPV1_and_TRPA1_Channels_Are_Both_Involved_Downstream_of_Histamine_Induced_Itch_ L2 - https://www.mdpi.com/resolver?pii=biom11081166 DB - PRIME DP - Unbound Medicine ER -