Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples.
Molecules. 2021 Aug 14; 26(16)M

Abstract

In this study, we propose a simple, cost-effective, and sensitive high-performance liquid chromatography with fluorescence detection (HPLC-FLD) for the simultaneous determination of seven bisphenols (bisphenol F (BPF), bisphenol E (BPE), bisphenol B (BPB), BADGE (bisphenol A diglycidyl ether), BADGE∙2H2O, BADGE∙H2O, BADGE∙2HCl) in human breast milk samples. The dispersive solid phase extraction (d-SPE) coupled with solid phase extraction (SPE) procedure performed well for the majority of the analytes with recoveries in the range 57-88% and relative standard deviations (RSD%) of less than 9.4%. During the d-SPE stage, no significant matrix effect was observed thanks to the application of different pairs of salts such as zirconium-dioxide-based sorbents (Z-Sep or Z-Sep +) and primary secondary amine (PSA) or QuEChERS Enhanced Matrix Removal-Lipid (EMR-Lipid) and PSA. The method limits of quantification (mLOQs) for all investigated analytes were set at satisfactory low values in the range 171.89-235.11 ng mL-1. Analyte concentrations were determined as the average value from human breast milk matrix samples. The results show that the d-SPE/SPE procedure, especially with the application of EMR-Lipid and PSA, could be used for further bisphenol analyses in human breast milk samples.

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Authors+Show Affiliations

Tuzimski T

Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.

Szubartowski S

Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland. Doctoral School of Medical University of Lublin, Medical University of Lublin, Chodźki 7, 20-093 Lublin, Poland.

MeSH

AminesBenzhydryl CompoundsChromatography, High Pressure LiquidFemaleFood ContaminationHumansMilk, HumanPhenolsSolid Phase ExtractionTandem Mass Spectrometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34443517

Citation

Tuzimski, Tomasz, and Szymon Szubartowski. "Application of d-SPE Before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples." Molecules (Basel, Switzerland), vol. 26, no. 16, 2021.

Tuzimski T, Szubartowski S. Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples. Molecules. 2021;26(16).

Tuzimski, T., & Szubartowski, S. (2021). Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples. Molecules (Basel, Switzerland), 26(16). https://doi.org/10.3390/molecules26164930

Tuzimski T, Szubartowski S. Application of d-SPE Before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples. Molecules. 2021 Aug 14;26(16) PubMed PMID: 34443517.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples. AU - Tuzimski,Tomasz, AU - Szubartowski,Szymon, Y1 - 2021/08/14/ PY - 2021/06/30/received PY - 2021/08/10/revised PY - 2021/08/12/accepted PY - 2021/8/27/entrez PY - 2021/8/28/pubmed PY - 2021/9/25/medline KW - BADGE (bisphenol A diglycidyl ether) and its derivatives KW - bisphenols KW - dispersive solid phase extraction (d-SPE)/solid phase extraction (SPE) KW - fluorescence detector (FLD) KW - high-performance liquid chromatography (HPLC) KW - human breast milk samples JF - Molecules (Basel, Switzerland) JO - Molecules VL - 26 IS - 16 N2 - In this study, we propose a simple, cost-effective, and sensitive high-performance liquid chromatography with fluorescence detection (HPLC-FLD) for the simultaneous determination of seven bisphenols (bisphenol F (BPF), bisphenol E (BPE), bisphenol B (BPB), BADGE (bisphenol A diglycidyl ether), BADGE∙2H2O, BADGE∙H2O, BADGE∙2HCl) in human breast milk samples. The dispersive solid phase extraction (d-SPE) coupled with solid phase extraction (SPE) procedure performed well for the majority of the analytes with recoveries in the range 57-88% and relative standard deviations (RSD%) of less than 9.4%. During the d-SPE stage, no significant matrix effect was observed thanks to the application of different pairs of salts such as zirconium-dioxide-based sorbents (Z-Sep or Z-Sep +) and primary secondary amine (PSA) or QuEChERS Enhanced Matrix Removal-Lipid (EMR-Lipid) and PSA. The method limits of quantification (mLOQs) for all investigated analytes were set at satisfactory low values in the range 171.89-235.11 ng mL-1. Analyte concentrations were determined as the average value from human breast milk matrix samples. The results show that the d-SPE/SPE procedure, especially with the application of EMR-Lipid and PSA, could be used for further bisphenol analyses in human breast milk samples. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/34443517/Application_of_d_SPE_before_SPE_and_HPLC_FLD_to_Analyze_Bisphenols_in_Human_Breast_Milk_Samples_ DB - PRIME DP - Unbound Medicine ER -

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Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples.Molecules. 2021 Aug 14; 26(16)M