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Assessment of diagnostic potential of some circulating microRNAs in Amyotrophic Lateral Sclerosis Patients, an Egyptian study.
Clin Neurol Neurosurg. 2021 Sep; 208:106883.CN

Abstract

OBJECTIVE

Numerous studies have been carried out to identify the role of microRNA (miRNA) as potential biomarkers for many diseases including amyotrophic lateral sclerosis (ALS). The aim of this study was to explore the circulating levels of some miRNAs in cohort of Egyptian ALS patients in an attempt to correlate the selected miRNA profiles with disease progression.

METHODS

Thirty ALS patients and 20 age and sex matched healthy controls were enrolled. Circulating miRNA levels were determined in venous blood samples, collected on EDTA, from all the study subjects. The selection of miRNA species (miR-206, miR-142-3p, miR-143-3p, miR-181a-5p, miR-106b-3p, miR-4516 and Let7f-5p) was based on their involvement in the pathophysiology of ALS and was further confirmed by data mining of specific miRNA databases (miRBase and miRDB).

RESULTS

As compared to the control group, significant consistent upregulation was found in the levels of miR-206, miR-143-3p and to a lesser extent in miR-142-3p. An elevation trend, although not significant, was also found in the levels of miR-181a-5p, miR-106b-3p, and miR-4516. Interestingly, we found that the levels of miR-142-3p were elevated in familial cases, while that of miR-4516 were significantly increased in sporadic cases. Furthermore, the levels of Let7f-5p, although were generally lowered in ALS patients but were also decreased in familial cases as well as in spinal onset ALS as compared to bulbar onset.

CONCLUSION

This is the first study investigating miRNA profiles in Egyptian ALS patients. We found that some miRNAs are significantly altered in ALS patients, and some may be used to distinguish familial and sporadic cases and bulbar and spinal onset. Larger study is needed, in which we will conduct a correlation of miRNA levels against variations in disease onset, progression as well as systemic inflammatory responses and the extent of neuromuscular involvement in Egyptian ALS patients in an attempt to identify environmental/occupational risk factors.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Soliman R
Neuromuscular Unit, Neurology and Psychiatry Department, Faculty of Medicine Ain Shams University, Cairo 11566, Egypt. Electronic address: radwasoliman@med.asu.edu.eg.
Mousa NO
Biotechnology Department, Basic and Applied Sciences Institute, Egypt-Japan University of Science and Technology, Borg Al Arab 21934, Egypt; Biotechnology Program, Chemistry Department, Faculty of Science, Cairo University, Cairo 12613, Egypt. Electronic address: nahlaosama@aucegypt.edu.
Rashed HR
Neuromuscular Unit, Neurology and Psychiatry Department, Faculty of Medicine Ain Shams University, Cairo 11566, Egypt. Electronic address: rashedheba@med.asu.edu.eg.
Moustafa RR
Neuromuscular Unit, Neurology and Psychiatry Department, Faculty of Medicine Ain Shams University, Cairo 11566, Egypt. Electronic address: ramezrm@med.asu.edu.eg.
Hamdi N
Molecular Pathology Unit, German University in Cairo (GUC), Cairo, Egypt. Electronic address: nabila.hamdi@guc.edu.eg.
Osman A
Biotechnology Department, Basic and Applied Sciences Institute, Egypt-Japan University of Science and Technology, Borg Al Arab 21934, Egypt; Biochemistry Department, Faculty of Science, Ain Shams University, Cairo 11566, Egypt. Electronic address: ahmed.osman@ejust.edu.eg.
Fahmy N
Neuromuscular Unit, Neurology and Psychiatry Department, Faculty of Medicine Ain Shams University, Cairo 11566, Egypt. Electronic address: dr.nagiafahmy@med.asu.edu.eg.

MeSH

AdultAgedAmyotrophic Lateral SclerosisBiomarkersCirculating MicroRNAEgyptFemaleHumansMaleMiddle Aged

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34454204

Citation

Soliman, Radwa, et al. "Assessment of Diagnostic Potential of some Circulating microRNAs in Amyotrophic Lateral Sclerosis Patients, an Egyptian Study." Clinical Neurology and Neurosurgery, vol. 208, 2021, p. 106883.
Soliman R, Mousa NO, Rashed HR, et al. Assessment of diagnostic potential of some circulating microRNAs in Amyotrophic Lateral Sclerosis Patients, an Egyptian study. Clin Neurol Neurosurg. 2021;208:106883.
Soliman, R., Mousa, N. O., Rashed, H. R., Moustafa, R. R., Hamdi, N., Osman, A., & Fahmy, N. (2021). Assessment of diagnostic potential of some circulating microRNAs in Amyotrophic Lateral Sclerosis Patients, an Egyptian study. Clinical Neurology and Neurosurgery, 208, 106883. https://doi.org/10.1016/j.clineuro.2021.106883
Soliman R, et al. Assessment of Diagnostic Potential of some Circulating microRNAs in Amyotrophic Lateral Sclerosis Patients, an Egyptian Study. Clin Neurol Neurosurg. 2021;208:106883. PubMed PMID: 34454204.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of diagnostic potential of some circulating microRNAs in Amyotrophic Lateral Sclerosis Patients, an Egyptian study. AU - Soliman,Radwa, AU - Mousa,Nahla O, AU - Rashed,Hebatallah R, AU - Moustafa,Ramez R, AU - Hamdi,Nabila, AU - Osman,Ahmad, AU - Fahmy,Nagia, Y1 - 2021/08/10/ PY - 2021/04/13/received PY - 2021/08/05/revised PY - 2021/08/06/accepted PY - 2021/8/29/pubmed PY - 2022/1/27/medline PY - 2021/8/28/entrez KW - ALS KW - miR-142-3p KW - miR-143-3p KW - miR-206 KW - miR-4516 SP - 106883 EP - 106883 JF - Clinical neurology and neurosurgery JO - Clin Neurol Neurosurg VL - 208 N2 - OBJECTIVE: Numerous studies have been carried out to identify the role of microRNA (miRNA) as potential biomarkers for many diseases including amyotrophic lateral sclerosis (ALS). The aim of this study was to explore the circulating levels of some miRNAs in cohort of Egyptian ALS patients in an attempt to correlate the selected miRNA profiles with disease progression. METHODS: Thirty ALS patients and 20 age and sex matched healthy controls were enrolled. Circulating miRNA levels were determined in venous blood samples, collected on EDTA, from all the study subjects. The selection of miRNA species (miR-206, miR-142-3p, miR-143-3p, miR-181a-5p, miR-106b-3p, miR-4516 and Let7f-5p) was based on their involvement in the pathophysiology of ALS and was further confirmed by data mining of specific miRNA databases (miRBase and miRDB). RESULTS: As compared to the control group, significant consistent upregulation was found in the levels of miR-206, miR-143-3p and to a lesser extent in miR-142-3p. An elevation trend, although not significant, was also found in the levels of miR-181a-5p, miR-106b-3p, and miR-4516. Interestingly, we found that the levels of miR-142-3p were elevated in familial cases, while that of miR-4516 were significantly increased in sporadic cases. Furthermore, the levels of Let7f-5p, although were generally lowered in ALS patients but were also decreased in familial cases as well as in spinal onset ALS as compared to bulbar onset. CONCLUSION: This is the first study investigating miRNA profiles in Egyptian ALS patients. We found that some miRNAs are significantly altered in ALS patients, and some may be used to distinguish familial and sporadic cases and bulbar and spinal onset. Larger study is needed, in which we will conduct a correlation of miRNA levels against variations in disease onset, progression as well as systemic inflammatory responses and the extent of neuromuscular involvement in Egyptian ALS patients in an attempt to identify environmental/occupational risk factors. SN - 1872-6968 UR - https://www.unboundmedicine.com/medline/citation/34454204/Assessment_of_diagnostic_potential_of_some_circulating_microRNAs_in_Amyotrophic_Lateral_Sclerosis_Patients_an_Egyptian_study_ DB - PRIME DP - Unbound Medicine ER -