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Embelia Laeta aqueous extract suppresses acute inflammation via decreasing COX-2/iNOS expression and inhibiting NF-κB pathway.
J Ethnopharmacol. 2021 Dec 05; 281:114575.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The root of Embelia laeta (L.) Mez., which is called Suanjifeng in Chinese ethnic Yao medicine, is traditionally for inflammation-related diseases, such as oral ulcer, sore throat, enteritis, and rheumatoid arthritis. However, the biological properties and the underlying mechanisms of Embelia laeta still need further studies.

AIM OF THIS STUDY

The present study aims to investigate the anti-inflammatory effect and its underlying mechanisms of Embelia laeta.

MATERIALS AND METHODS

In this study, except acute toxicity experiments, Kunming (KM) mice of either sex were enrolled to establish inflammatory model induced by xylene, acetic acid and carrageenan, respectively. Mice were randomly divided into different groups and pretreated by oral gavage with different doses of Embelia laeta aqueous extract (ELAE) (2.5, 5, 10 g/kg) and 10 mg/kg of Indo for 7 days. Ear edema, vascular permeability, abdominal writhing, and paw edema degree were detected in related experiments. Moreover, in the carrageenan-induced paw edema mice model, histological changes were detected by H&E staining. MDA, MPO and NO were detected by assay kit. Proinflammatory cytokines of IFN-γ, TNF-α, IL-1β, IL-6 and PGE2 were detected by ELISA. Additionally, COX-2, iNOS and NF-κB pathway-related proteins were detected by Western blotting.

RESULTS

Results showed that the ELAE evoked an obvious dose-dependent inhibition of ear edema induced by xylene, paw edema induced by carrageenan, as well as suppressing the increase of vascular permeability and writhing times elicited by acetic acid. Histopathological analysis indicated that ELAE could significantly decrease the cellular infiltration in paw tissue. ELAE showed antioxidant property through markedly decrease the MDA level and MPO activity in edema paw. In addition, ELAE decreased the proinflammatory cytokines IFN-γ, TNF-α, IL-1β, IL-6, PGE2 and NO that induced by carrageenan. Western blotting results also showed that ELAE could obviously downregulate the COX-2 and iNOS expression. Further analysis revealed that ELAE also inhibited NF-κB from the cytoplasm to the nucleus and stabilize the conversion of IκBα.

CONCLUSION

ELAE had powerful anti-inflammatory property, which might be had a close relationship with mediating proinflammatory cytokines production, decreasing the COX-2 and iNOS expression, and inhibiting the activation of NF-κB signaling pathway.

Authors+Show Affiliations

College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China. Electronic address: zzmfwt@qq.com.College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, China; College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China. Electronic address: 136067251@qq.com.Clinical Research Center, Shantou Central Hospital, Shantou, Guangdong, China. Electronic address: hgvxin@163.com.College of Pharmacy, Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning, China. Electronic address: 494997752@qq.com.College of Pharmacy, Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning, China. Electronic address: 3578316045@qq.com.College of Pharmacy, Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning, China. Electronic address: lxx.2015.0829@qq.com.College of Basic Medicine, Guangxi University of Chinese Medicine, Nanning, China. Electronic address: 416158716@qq.com.College of Basic Medicine, Guangxi University of Chinese Medicine, Nanning, China. Electronic address: 1713552545@qq.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34461190

Citation

Zhang, Zhongmin, et al. "Embelia Laeta Aqueous Extract Suppresses Acute Inflammation Via Decreasing COX-2/iNOS Expression and Inhibiting NF-κB Pathway." Journal of Ethnopharmacology, vol. 281, 2021, p. 114575.
Zhang Z, Li L, Huang G, et al. Embelia Laeta aqueous extract suppresses acute inflammation via decreasing COX-2/iNOS expression and inhibiting NF-κB pathway. J Ethnopharmacol. 2021;281:114575.
Zhang, Z., Li, L., Huang, G., Zhou, T., Zhang, X., Leng, X., Chen, Z., & Lin, J. (2021). Embelia Laeta aqueous extract suppresses acute inflammation via decreasing COX-2/iNOS expression and inhibiting NF-κB pathway. Journal of Ethnopharmacology, 281, 114575. https://doi.org/10.1016/j.jep.2021.114575
Zhang Z, et al. Embelia Laeta Aqueous Extract Suppresses Acute Inflammation Via Decreasing COX-2/iNOS Expression and Inhibiting NF-κB Pathway. J Ethnopharmacol. 2021 Dec 5;281:114575. PubMed PMID: 34461190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Embelia Laeta aqueous extract suppresses acute inflammation via decreasing COX-2/iNOS expression and inhibiting NF-κB pathway. AU - Zhang,Zhongmin, AU - Li,Li, AU - Huang,Guoxin, AU - Zhou,Tong, AU - Zhang,Xinyue, AU - Leng,Xinxin, AU - Chen,Zhenxing, AU - Lin,Jiang, Y1 - 2021/08/27/ PY - 2021/04/07/received PY - 2021/08/22/revised PY - 2021/08/26/accepted PY - 2021/8/31/pubmed PY - 2021/8/31/medline PY - 2021/8/30/entrez KW - Embelia laeta KW - Inflammation KW - Mechanism KW - NF-κB KW - Traditional Chinese medicine SP - 114575 EP - 114575 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 281 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The root of Embelia laeta (L.) Mez., which is called Suanjifeng in Chinese ethnic Yao medicine, is traditionally for inflammation-related diseases, such as oral ulcer, sore throat, enteritis, and rheumatoid arthritis. However, the biological properties and the underlying mechanisms of Embelia laeta still need further studies. AIM OF THIS STUDY: The present study aims to investigate the anti-inflammatory effect and its underlying mechanisms of Embelia laeta. MATERIALS AND METHODS: In this study, except acute toxicity experiments, Kunming (KM) mice of either sex were enrolled to establish inflammatory model induced by xylene, acetic acid and carrageenan, respectively. Mice were randomly divided into different groups and pretreated by oral gavage with different doses of Embelia laeta aqueous extract (ELAE) (2.5, 5, 10 g/kg) and 10 mg/kg of Indo for 7 days. Ear edema, vascular permeability, abdominal writhing, and paw edema degree were detected in related experiments. Moreover, in the carrageenan-induced paw edema mice model, histological changes were detected by H&E staining. MDA, MPO and NO were detected by assay kit. Proinflammatory cytokines of IFN-γ, TNF-α, IL-1β, IL-6 and PGE2 were detected by ELISA. Additionally, COX-2, iNOS and NF-κB pathway-related proteins were detected by Western blotting. RESULTS: Results showed that the ELAE evoked an obvious dose-dependent inhibition of ear edema induced by xylene, paw edema induced by carrageenan, as well as suppressing the increase of vascular permeability and writhing times elicited by acetic acid. Histopathological analysis indicated that ELAE could significantly decrease the cellular infiltration in paw tissue. ELAE showed antioxidant property through markedly decrease the MDA level and MPO activity in edema paw. In addition, ELAE decreased the proinflammatory cytokines IFN-γ, TNF-α, IL-1β, IL-6, PGE2 and NO that induced by carrageenan. Western blotting results also showed that ELAE could obviously downregulate the COX-2 and iNOS expression. Further analysis revealed that ELAE also inhibited NF-κB from the cytoplasm to the nucleus and stabilize the conversion of IκBα. CONCLUSION: ELAE had powerful anti-inflammatory property, which might be had a close relationship with mediating proinflammatory cytokines production, decreasing the COX-2 and iNOS expression, and inhibiting the activation of NF-κB signaling pathway. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/34461190/Embelia_Laeta_aqueous_extract_suppresses_acute_inflammation_via_decreasing_COX_2/iNOS_expression_and_inhibiting_NF_κB_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(21)00804-7 DB - PRIME DP - Unbound Medicine ER -