TY - JOUR T1 - Exploring the sulfate patterns of chondroitin sulfate/dermatan sulfate and keratan sulfate in human pancreatic cancer. AU - Ren,Qiang, AU - Wang,Jian, AU - Liu,Chao, AU - Meng,Ling-Xin, AU - Qian,Rui-Kun, AU - Gao,Hui-Jie, AU - Qin,Wei, AU - Zhou,Cai-Ju, AU - Qiao,Sen, AU - Wang,Hui-Yun, AU - Zhang,Li-Tao, AU - Zhang,Yun-Tao, Y1 - 2021/08/26/ PY - 2021/05/23/received PY - 2021/08/16/revised PY - 2021/08/21/accepted PY - 2021/9/1/pubmed PY - 2021/10/6/medline PY - 2021/8/31/entrez KW - Carbohydrate sulfotransferases KW - Chondroitin sulfate KW - Keratan sulfate KW - LC–MS/MS KW - Pancreatic cancer SP - 114339 EP - 114339 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 205 N2 - This study was designed to explore the sulfation patterns of chondroitin sulfate (CS)/dermatan sulfate (DS), and keratan sulfate (KS) and the expression of carbohydrate sulfotransferases (CHSTs) in 26 pancreatic tumor and normal tissues. CS/DS and KS profiles were simultaneously determined. Pancreatic tumor tissues exhibited increased ΔDi-0S, ΔDi-4S, and ΔDi-6S levels, with absolute ΔDi-4S content being highest, followed by ΔDi-6S. However, as for the contents of KS-6S and KS-6S,6'S, there were no significant regular change. The expression levels of CHST1 and CHST4 were 37 and 15 times higher than those in normal tissues. PCA and OPLS-DA revealed that ΔDi-4S and ΔDi-6S levels could be reliably used to differentiate between healthy and cancerous tissues. The up-regulation of CHST3, CHST12, CHST13, and CHST15 was directly correlated with C-4 and C-6 sulfation. These data provide a foundation for future studies of the role of ΔDi-4S and ΔDi-6S in the progression of pancreatic cancer. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/34464868/Exploring_the_sulfate_patterns_of_chondroitin_sulfate/dermatan_sulfate_and_keratan_sulfate_in_human_pancreatic_cancer_ DB - PRIME DP - Unbound Medicine ER -