TY - JOUR T1 - Surface plasmon resonance aptasensor based on niobium carbide MXene quantum dots for nucleocapsid of SARS-CoV-2 detection. AU - Chen,Rongyuan, AU - Kan,Lun, AU - Duan,Fenghe, AU - He,Linghao, AU - Wang,Minghua, AU - Cui,Jing, AU - Zhang,Zhihong, AU - Zhang,Zhonghou, Y1 - 2021/09/02/ PY - 2021/04/21/received PY - 2021/08/11/accepted PY - 2021/9/3/entrez PY - 2021/9/4/pubmed PY - 2021/9/15/medline KW - Aptasensor KW - Detection of N-gene KW - Nb2C MXene quantum dot KW - SARS-CoV-2 KW - Surface plasmon resonance biosensor SP - 316 EP - 316 JF - Mikrochimica acta JO - Mikrochim Acta VL - 188 IS - 10 N2 - A novel label-free surface plasmon resonance (SPR) aptasensor has been constructed for the detection of N-gene of SARS-CoV-2 by using thiol-modified niobium carbide MXene quantum dots (Nb2C-SH QDs) as the bioplatform for anchoring N-gene-targeted aptamer. In the presence of SARS-CoV-2 N-gene, the immobilized aptamer strands changed their conformation to specifically bind with N-gene. It thus increased the contact area or enlarged the distance between aptamer and the SPR chip, resulting in a change of the SPR signal irradiated by the laser (He-Ne) with the wavelength (λ) of 633 nm. Nb2C QDs were derived from Nb2C MXene nanosheets via a solvothermal method, followed by functionalization with octadecanethiol through a self-assembling method. Subsequently, the gold chip for SPR measurements was modified with Nb2C-SH QDs via covalent binding of the Au-S bond also by self-assembling interaction. Nb2C-SH QDs not only resulted in high bioaffinity toward aptamer but also enhanced the SPR response. Thus, the Nb2C-SH QD-based SPR aptasensor had low limit of detection (LOD) of 4.9 pg mL-1 toward N-gene within the concentration range 0.05 to 100 ng mL-1. The sensor also showed excellent selectivity in the presence of various respiratory viruses and proteins in human serum and high stability. Moreover, the Nb2C-SH QD-based SPR aptasensor displayed a vast practical application for the qualitative analysis of N-gene from different samples, including seawater, seafood, and human serum. Thus, this work can provide a deep insight into the construction of the aptasensor for detecting SARS-CoV-2 in complex environments. A novel label-free surface plasmon resonance aptasensor has been constructed to detect sensitively and selectively the N-gene of SARS-CoV-2 by using thiol-modified niobium carbide MXene quantum dots as the scaffold to anchor the N-gene-targeted aptamer. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/34476615/Surface_plasmon_resonance_aptasensor_based_on_niobium_carbide_MXene_quantum_dots_for_nucleocapsid_of_SARS_CoV_2_detection_ DB - PRIME DP - Unbound Medicine ER -