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Genome-wide identification and characterization of bZIP transcription factors and their expression profile under abiotic stresses in Chinese pear (Pyrus bretschneideri).
BMC Plant Biol. 2021 Sep 09; 21(1):413.BP

Abstract

BACKGROUND

In plants, basic leucine zipper transcription factors (TFs) play important roles in multiple biological processes such as anthesis, fruit growth & development and stress responses. However, systematic investigation and characterization of bZIP-TFs remain unclear in Chinese white pear. Chinese white pear is a fruit crop that has important nutritional and medicinal values.

RESULTS

In this study, 62 bZIP genes were comprehensively identified from Chinese Pear, and 54 genes were distributed among 17 chromosomes. Frequent whole-genome duplication (WGD) and dispersed duplication (DSD) were the major driving forces underlying the bZIP gene family in Chinese white pear. bZIP-TFs are classified into 13 subfamilies according to the phylogenetic tree. Subsequently, purifying selection plays an important role in the evolution process of PbbZIPs. Synteny analysis of bZIP genes revealed that 196 orthologous gene pairs were identified between Pyrus bretschneideri, Fragaria vesca, Prunus mume, and Prunus persica. Moreover, cis-elements that respond to various stresses and hormones were found on the promoter regions of PbbZIP, which were induced by stimuli. Gene structure (intron/exon) and different compositions of motifs revealed that functional divergence among subfamilies. Expression pattern of PbbZIP genes differential expressed under hormonal treatment abscisic acid, salicylic acid, and methyl jasmonate in pear fruits by real-time qRT-PCR.

CONCLUSIONS

Collectively, a systematic analysis of gene structure, motif composition, subcellular localization, synteny analysis, and calculation of synonymous (Ks) and non-synonymous (Ka) was performed in Chinese white pear. Sixty-two bZIP-TFs in Chinese pear were identified, and their expression profiles were comprehensively analyzed under ABA, SA, and MeJa hormones, which respond to multiple abiotic stresses and fruit growth and development. PbbZIP gene occurred through Whole-genome duplication and dispersed duplication events. These results provide a basic framework for further elucidating the biological function characterizations under multiple developmental stages and abiotic stress responses.

Authors+Show Affiliations

School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.Department of Software Engineering, University of Gujrat, Lahore, 54000, Pakistan.School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.Department of Environment and Soil Sciences, University of Lleida, Avinguda Alcalde Rovira Roure 191, 25198, Lleida, Spain.College of Agronomy, Anhui Agricultural University, Hefei, 230036, China.Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, China.School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China. ypcaiah@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34503442

Citation

Manzoor, Muhammad Aamir, et al. "Genome-wide Identification and Characterization of bZIP Transcription Factors and Their Expression Profile Under Abiotic Stresses in Chinese Pear (Pyrus Bretschneideri)." BMC Plant Biology, vol. 21, no. 1, 2021, p. 413.
Manzoor MA, Manzoor MM, Li G, et al. Genome-wide identification and characterization of bZIP transcription factors and their expression profile under abiotic stresses in Chinese pear (Pyrus bretschneideri). BMC Plant Biol. 2021;21(1):413.
Manzoor, M. A., Manzoor, M. M., Li, G., Abdullah, M., Han, W., Wenlong, H., Shakoor, A., Riaz, M. W., Rehman, S., & Cai, Y. (2021). Genome-wide identification and characterization of bZIP transcription factors and their expression profile under abiotic stresses in Chinese pear (Pyrus bretschneideri). BMC Plant Biology, 21(1), 413. https://doi.org/10.1186/s12870-021-03191-3
Manzoor MA, et al. Genome-wide Identification and Characterization of bZIP Transcription Factors and Their Expression Profile Under Abiotic Stresses in Chinese Pear (Pyrus Bretschneideri). BMC Plant Biol. 2021 Sep 9;21(1):413. PubMed PMID: 34503442.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide identification and characterization of bZIP transcription factors and their expression profile under abiotic stresses in Chinese pear (Pyrus bretschneideri). AU - Manzoor,Muhammad Aamir, AU - Manzoor,Muhammad Mudassar, AU - Li,Guohui, AU - Abdullah,Muhammad, AU - Han,Wang, AU - Wenlong,Han, AU - Shakoor,Awais, AU - Riaz,Muhammad Waheed, AU - Rehman,Shamsur, AU - Cai,Yongping, Y1 - 2021/09/09/ PY - 2021/04/10/received PY - 2021/08/28/accepted PY - 2021/9/10/entrez PY - 2021/9/11/pubmed PY - 2021/9/21/medline KW - Expansion KW - Expression analysis KW - PbbZIP gene family KW - Phylogenetic analysis KW - Stress response SP - 413 EP - 413 JF - BMC plant biology JO - BMC Plant Biol VL - 21 IS - 1 N2 - BACKGROUND: In plants, basic leucine zipper transcription factors (TFs) play important roles in multiple biological processes such as anthesis, fruit growth & development and stress responses. However, systematic investigation and characterization of bZIP-TFs remain unclear in Chinese white pear. Chinese white pear is a fruit crop that has important nutritional and medicinal values. RESULTS: In this study, 62 bZIP genes were comprehensively identified from Chinese Pear, and 54 genes were distributed among 17 chromosomes. Frequent whole-genome duplication (WGD) and dispersed duplication (DSD) were the major driving forces underlying the bZIP gene family in Chinese white pear. bZIP-TFs are classified into 13 subfamilies according to the phylogenetic tree. Subsequently, purifying selection plays an important role in the evolution process of PbbZIPs. Synteny analysis of bZIP genes revealed that 196 orthologous gene pairs were identified between Pyrus bretschneideri, Fragaria vesca, Prunus mume, and Prunus persica. Moreover, cis-elements that respond to various stresses and hormones were found on the promoter regions of PbbZIP, which were induced by stimuli. Gene structure (intron/exon) and different compositions of motifs revealed that functional divergence among subfamilies. Expression pattern of PbbZIP genes differential expressed under hormonal treatment abscisic acid, salicylic acid, and methyl jasmonate in pear fruits by real-time qRT-PCR. CONCLUSIONS: Collectively, a systematic analysis of gene structure, motif composition, subcellular localization, synteny analysis, and calculation of synonymous (Ks) and non-synonymous (Ka) was performed in Chinese white pear. Sixty-two bZIP-TFs in Chinese pear were identified, and their expression profiles were comprehensively analyzed under ABA, SA, and MeJa hormones, which respond to multiple abiotic stresses and fruit growth and development. PbbZIP gene occurred through Whole-genome duplication and dispersed duplication events. These results provide a basic framework for further elucidating the biological function characterizations under multiple developmental stages and abiotic stress responses. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/34503442/Genome_wide_identification_and_characterization_of_bZIP_transcription_factors_and_their_expression_profile_under_abiotic_stresses_in_Chinese_pear__Pyrus_bretschneideri__ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-021-03191-3 DB - PRIME DP - Unbound Medicine ER -