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Impaired macrophages and failure of steroidogenesis and spermatogenesis in rat testes with cytokines deficiency induced by diacerein.
Histochem Cell Biol. 2021 Dec; 156(6):561-581.HC

Abstract

The role of cytokines in testicular function under normal conditions has not been completely understood. Here, we evaluated testicular macrophages (TM), steroidogenesis by Leydig cells (LC) and seminiferous tubules integrity in cytokines-deficient rat testes induced by diacerein, an anti-inflammatory drug that inhibits interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-α). Male rats received daily 100 mg/kg of diacerein (DIAG; n = 8) or saline (CG; n = 8) for 30 days. Serum testosterone (T) levels were measured and the seminiferous tubule (ST) area, epithelial area (EA), frequency of damaged ST and number of Sertoli cells (SC) were evaluated. TUNEL method and immunoreactions for detection of pro-IL-1β, TNF-α, steroidogenic acute regulatory protein (StAR), 17β-hydroxysteroid dehydrogenase (17β-HSD), androgen receptor (AR) and scavenger receptor for hemoglobin-haptoglobin complexes (CD163), a TM marker, were performed. Testicular AR, 17β-HSD and IL-1β levels were detected by Western blot. Data were submitted to Student t test (p ≤ 0.05). In DIAG, T and testicular AR, 17β-HSD and IL-1β levels decreased significantly (p < 0.05). The number of TUNEL-positive interstitial cells increased and LC showed weak StAR, 17β-HSD and AR immunoexpression in association with reduced IL-1β immunoexpression and number of CD163-positive TM in the interstitial tissue from diacerein-treated rats. Numerous damaged ST were found in DIAG, and reduction in the EA were associated with germ cells death. Moreover, the number of SC reduced and weak AR and TNF-α immunoexpression was observed in SC and germ cells, respectively. The cytokines deficiency induced by diacerein impairs TM, LC and spermatogenesis, and points to a role of IL-1β in steroidogenesis under normal conditions. In the ST, the weak AR and TNF-α immunoexpression in SC and germ cells, respectively, reinforces the idea that TNF-α plays a role in the SC androgenic control.

Authors+Show Affiliations

Department of Morphology and Genetics, Federal University of São Paulo, São Paulo, Brazil.Laboratory of Histology and Embryology, Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, Dental School - São Paulo State University (UNESP), Rua Humaitá, 1680, Araraquara, SP, CEP: 14801-903, Brazil.Laboratory of Histology and Embryology, Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, Dental School - São Paulo State University (UNESP), Rua Humaitá, 1680, Araraquara, SP, CEP: 14801-903, Brazil. estela.sasso@unesp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34515835

Citation

de Oliveira, Salmo Azambuja, et al. "Impaired Macrophages and Failure of Steroidogenesis and Spermatogenesis in Rat Testes With Cytokines Deficiency Induced By Diacerein." Histochemistry and Cell Biology, vol. 156, no. 6, 2021, pp. 561-581.
de Oliveira SA, Cerri PS, Sasso-Cerri E. Impaired macrophages and failure of steroidogenesis and spermatogenesis in rat testes with cytokines deficiency induced by diacerein. Histochem Cell Biol. 2021;156(6):561-581.
de Oliveira, S. A., Cerri, P. S., & Sasso-Cerri, E. (2021). Impaired macrophages and failure of steroidogenesis and spermatogenesis in rat testes with cytokines deficiency induced by diacerein. Histochemistry and Cell Biology, 156(6), 561-581. https://doi.org/10.1007/s00418-021-02023-7
de Oliveira SA, Cerri PS, Sasso-Cerri E. Impaired Macrophages and Failure of Steroidogenesis and Spermatogenesis in Rat Testes With Cytokines Deficiency Induced By Diacerein. Histochem Cell Biol. 2021;156(6):561-581. PubMed PMID: 34515835.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impaired macrophages and failure of steroidogenesis and spermatogenesis in rat testes with cytokines deficiency induced by diacerein. AU - de Oliveira,Salmo Azambuja, AU - Cerri,Paulo Sérgio, AU - Sasso-Cerri,Estela, Y1 - 2021/09/13/ PY - 2021/08/08/accepted PY - 2021/9/14/pubmed PY - 2022/3/9/medline PY - 2021/9/13/entrez KW - Anti-inflammatory KW - IL-1 KW - Leydig cell KW - Macrophage KW - Spermatogenesis KW - TNF-α SP - 561 EP - 581 JF - Histochemistry and cell biology JO - Histochem Cell Biol VL - 156 IS - 6 N2 - The role of cytokines in testicular function under normal conditions has not been completely understood. Here, we evaluated testicular macrophages (TM), steroidogenesis by Leydig cells (LC) and seminiferous tubules integrity in cytokines-deficient rat testes induced by diacerein, an anti-inflammatory drug that inhibits interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-α). Male rats received daily 100 mg/kg of diacerein (DIAG; n = 8) or saline (CG; n = 8) for 30 days. Serum testosterone (T) levels were measured and the seminiferous tubule (ST) area, epithelial area (EA), frequency of damaged ST and number of Sertoli cells (SC) were evaluated. TUNEL method and immunoreactions for detection of pro-IL-1β, TNF-α, steroidogenic acute regulatory protein (StAR), 17β-hydroxysteroid dehydrogenase (17β-HSD), androgen receptor (AR) and scavenger receptor for hemoglobin-haptoglobin complexes (CD163), a TM marker, were performed. Testicular AR, 17β-HSD and IL-1β levels were detected by Western blot. Data were submitted to Student t test (p ≤ 0.05). In DIAG, T and testicular AR, 17β-HSD and IL-1β levels decreased significantly (p < 0.05). The number of TUNEL-positive interstitial cells increased and LC showed weak StAR, 17β-HSD and AR immunoexpression in association with reduced IL-1β immunoexpression and number of CD163-positive TM in the interstitial tissue from diacerein-treated rats. Numerous damaged ST were found in DIAG, and reduction in the EA were associated with germ cells death. Moreover, the number of SC reduced and weak AR and TNF-α immunoexpression was observed in SC and germ cells, respectively. The cytokines deficiency induced by diacerein impairs TM, LC and spermatogenesis, and points to a role of IL-1β in steroidogenesis under normal conditions. In the ST, the weak AR and TNF-α immunoexpression in SC and germ cells, respectively, reinforces the idea that TNF-α plays a role in the SC androgenic control. SN - 1432-119X UR - https://www.unboundmedicine.com/medline/citation/34515835/Impaired_macrophages_and_failure_of_steroidogenesis_and_spermatogenesis_in_rat_testes_with_cytokines_deficiency_induced_by_diacerein_ L2 - https://dx.doi.org/10.1007/s00418-021-02023-7 DB - PRIME DP - Unbound Medicine ER -