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Microbiota-Macroalgal Relationships at a Hawaiian Intertidal Bench Are Influenced by Macroalgal Phyla and Associated Thallus Complexity.
mSphere. 2021 10 27; 6(5):e0066521.M

Abstract

The ocean represents the largest biome on earth; however, we have only begun to understand the diversity and function of the marine microbial inhabitants and their interactions with macroalgal species. Macroalgae play an integral role in overall ocean biome health and serve both as major primary producers and foundation species in the ecosystem. Previous studies have been limited, focusing on the microbiome of a single algal species or its interaction with selected microbes. This project aimed to understand overall biodiversity of microbial communities associated with five common macroalgal species and to determine the drivers of these communities at 'Ewa Beach, O'ahu, HI. Representative species of Chlorophyta (green), Ochrophyta (brown), and Rhodophyta (red) algae, each species having various levels of calcification, thallus complexity, and status as native or invasive species, were collected from an intertidal bench in May 2019. A portion of the V3-V4 variable region of the small-subunit rRNA gene was amplified for high-throughput sequencing using universal bacterial primers to elucidate the core and variable algal microbiome. Significant differences in bacterial community composition were only partially explained by host species, whether the host was native or invasive, and thallus complexity. Macroalgal phylum explained the most variation in associated microbial communities at 'Ewa Beach. This study advances our understanding of microbial-macroalgal interactions and their connectivity by producing insight into factors that influence the community structure of macroalga-associated microbiota. IMPORTANCE Generally, most eukaryotic organisms form relationships with microbes that are important in mediating host organismal health. Macroalgae are a diverse group of photosynthetic eukaryotic organisms that serve as primary producers and foundational species in many ecosystems. However, little is known about their microbial counterparts across a wide range of macroalgal morphologies, phylogenies, and calcification levels. Thus, to further understand the factors involved in bacterial community composition associated with macroalgal species at one point in time, representative samples were collected across phyla. Here, we show that both host macroalga phyla and morphology influenced the associated microbial community. Additionally, we show that the invasive species Avrainvillea lacerata does not have a unique microbial community on this intertidal bench, further supporting the idea that host phylum strongly influences microbial community composition.

Authors+Show Affiliations

Department of Biology, College of Charleston, Charleston, South Carolina, USA.Department of Biology, College of Charleston, Charleston, South Carolina, USA.Department of Biology, Grice Marine Lab, College of Charleston, Charleston, South Carolina, USA.Department of Biology, College of Charleston, Charleston, South Carolina, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

34550007

Citation

Kuba, Gabrielle M., et al. "Microbiota-Macroalgal Relationships at a Hawaiian Intertidal Bench Are Influenced By Macroalgal Phyla and Associated Thallus Complexity." MSphere, vol. 6, no. 5, 2021, pp. e0066521.
Kuba GM, Spalding HL, Hill-Spanik KM, et al. Microbiota-Macroalgal Relationships at a Hawaiian Intertidal Bench Are Influenced by Macroalgal Phyla and Associated Thallus Complexity. mSphere. 2021;6(5):e0066521.
Kuba, G. M., Spalding, H. L., Hill-Spanik, K. M., & Fullerton, H. (2021). Microbiota-Macroalgal Relationships at a Hawaiian Intertidal Bench Are Influenced by Macroalgal Phyla and Associated Thallus Complexity. MSphere, 6(5), e0066521. https://doi.org/10.1128/mSphere.00665-21
Kuba GM, et al. Microbiota-Macroalgal Relationships at a Hawaiian Intertidal Bench Are Influenced By Macroalgal Phyla and Associated Thallus Complexity. mSphere. 2021 10 27;6(5):e0066521. PubMed PMID: 34550007.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microbiota-Macroalgal Relationships at a Hawaiian Intertidal Bench Are Influenced by Macroalgal Phyla and Associated Thallus Complexity. AU - Kuba,Gabrielle M, AU - Spalding,Heather L, AU - Hill-Spanik,Kristina M, AU - Fullerton,Heather, Y1 - 2021/09/22/ PY - 2021/9/23/pubmed PY - 2022/2/1/medline PY - 2021/9/22/entrez KW - invasive algae KW - macroalgae KW - microbiome KW - native algae SP - e0066521 EP - e0066521 JF - mSphere JO - mSphere VL - 6 IS - 5 N2 - The ocean represents the largest biome on earth; however, we have only begun to understand the diversity and function of the marine microbial inhabitants and their interactions with macroalgal species. Macroalgae play an integral role in overall ocean biome health and serve both as major primary producers and foundation species in the ecosystem. Previous studies have been limited, focusing on the microbiome of a single algal species or its interaction with selected microbes. This project aimed to understand overall biodiversity of microbial communities associated with five common macroalgal species and to determine the drivers of these communities at 'Ewa Beach, O'ahu, HI. Representative species of Chlorophyta (green), Ochrophyta (brown), and Rhodophyta (red) algae, each species having various levels of calcification, thallus complexity, and status as native or invasive species, were collected from an intertidal bench in May 2019. A portion of the V3-V4 variable region of the small-subunit rRNA gene was amplified for high-throughput sequencing using universal bacterial primers to elucidate the core and variable algal microbiome. Significant differences in bacterial community composition were only partially explained by host species, whether the host was native or invasive, and thallus complexity. Macroalgal phylum explained the most variation in associated microbial communities at 'Ewa Beach. This study advances our understanding of microbial-macroalgal interactions and their connectivity by producing insight into factors that influence the community structure of macroalga-associated microbiota. IMPORTANCE Generally, most eukaryotic organisms form relationships with microbes that are important in mediating host organismal health. Macroalgae are a diverse group of photosynthetic eukaryotic organisms that serve as primary producers and foundational species in many ecosystems. However, little is known about their microbial counterparts across a wide range of macroalgal morphologies, phylogenies, and calcification levels. Thus, to further understand the factors involved in bacterial community composition associated with macroalgal species at one point in time, representative samples were collected across phyla. Here, we show that both host macroalga phyla and morphology influenced the associated microbial community. Additionally, we show that the invasive species Avrainvillea lacerata does not have a unique microbial community on this intertidal bench, further supporting the idea that host phylum strongly influences microbial community composition. SN - 2379-5042 UR - https://www.unboundmedicine.com/medline/citation/34550007/Microbiota_Macroalgal_Relationships_at_a_Hawaiian_Intertidal_Bench_Are_Influenced_by_Macroalgal_Phyla_and_Associated_Thallus_Complexity_ DB - PRIME DP - Unbound Medicine ER -