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A defined system for in vitro packaging of DNA-gp3 of the Bacillus subtilis bacteriophage phi 29.
Proc Natl Acad Sci U S A 1986; 83(10):3505-9PN

Abstract

The bacteriophage phi 29 DNA-gene product 3 complex (DNA-gp3) has been efficiently packaged into proheads in a completely defined in vitro system. The phi 29 DNA packaging protein gp16, the product of gene 16, was overproduced in Escherichia coli and purified to near homogeneity. The purified gp16 packaged 23% of the DNA-gp3 added to purified proheads in the defined mixture, while gp16 in an extract of phage-infected cells packaged 26% of the DNA-gp3. No host proteins were required in the defined system. ATP-dependent packaging of DNA-gp3 in the defined system was optimal with approximately equal to 100 copies of gp16 per DNA-gp3, an amount similar to the production of gp16 per DNA-gp3 in phi 29-infected cells.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3458193

Citation

Guo, P, et al. "A Defined System for in Vitro Packaging of DNA-gp3 of the Bacillus Subtilis Bacteriophage Phi 29." Proceedings of the National Academy of Sciences of the United States of America, vol. 83, no. 10, 1986, pp. 3505-9.
Guo P, Grimes S, Anderson D. A defined system for in vitro packaging of DNA-gp3 of the Bacillus subtilis bacteriophage phi 29. Proc Natl Acad Sci USA. 1986;83(10):3505-9.
Guo, P., Grimes, S., & Anderson, D. (1986). A defined system for in vitro packaging of DNA-gp3 of the Bacillus subtilis bacteriophage phi 29. Proceedings of the National Academy of Sciences of the United States of America, 83(10), pp. 3505-9.
Guo P, Grimes S, Anderson D. A Defined System for in Vitro Packaging of DNA-gp3 of the Bacillus Subtilis Bacteriophage Phi 29. Proc Natl Acad Sci USA. 1986;83(10):3505-9. PubMed PMID: 3458193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A defined system for in vitro packaging of DNA-gp3 of the Bacillus subtilis bacteriophage phi 29. AU - Guo,P, AU - Grimes,S, AU - Anderson,D, PY - 1986/5/1/pubmed PY - 1986/5/1/medline PY - 1986/5/1/entrez SP - 3505 EP - 9 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 83 IS - 10 N2 - The bacteriophage phi 29 DNA-gene product 3 complex (DNA-gp3) has been efficiently packaged into proheads in a completely defined in vitro system. The phi 29 DNA packaging protein gp16, the product of gene 16, was overproduced in Escherichia coli and purified to near homogeneity. The purified gp16 packaged 23% of the DNA-gp3 added to purified proheads in the defined mixture, while gp16 in an extract of phage-infected cells packaged 26% of the DNA-gp3. No host proteins were required in the defined system. ATP-dependent packaging of DNA-gp3 in the defined system was optimal with approximately equal to 100 copies of gp16 per DNA-gp3, an amount similar to the production of gp16 per DNA-gp3 in phi 29-infected cells. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/3458193/A_defined_system_for_in_vitro_packaging_of_DNA_gp3_of_the_Bacillus_subtilis_bacteriophage_phi_29_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=3458193 DB - PRIME DP - Unbound Medicine ER -