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Evaluation of Urinary Clusterin and Cystatin B as Biomarkers for Renal Injury in Dogs Envenomated by the European Adder (Vipera berus).
Top Companion Anim Med. 2022 Jan-Feb; 46:100586.TC

Abstract

Dogs are commonly bitten by the European adder (Vipera berus) but studies investigating the effects of envenomation are limited. Snakebite-related kidney injury is reported in dogs but diagnosis of acute kidney injury (AKI) might be limited by the insensitivity of routinely used renal function biomarkers. The aim of this study was to evaluate novel biomarkers of renal injury (urinary cystatin B and urinary clusterin) and biomarkers of renal function (serum creatinine and serum symmetric dimethylarginine), and urine protein to creatinine ratio in dogs envenomated by V. berus. Biomarkers were measured at presentation (T1), 12 hours (T2), 24 hours (T3), 36 hours (T4), and 14 days (T5) after snakebite and compared to a group of healthy control dogs. A secondary aim was to investigate the association between biomarker concentrations and severity of clinical signs of envenomation using a snakebite severity score (SSS). Urinary cystatin B concentrations were significantly higher at all timepoints in envenomated dogs compared to controls (P < .010), except for T5 (P = .222). Absolute urinary clusterin concentrations were not significantly different to controls at any timepoint. Compared to controls, serum creatinine and serum symmetric dimethylarginine concentrations were significantly lower in envenomated dogs at T1-T4 (P < .036) and T2-T4 (P < .036), respectively. Urine protein to creatinine ratio was higher in envenomated dogs compared to controls at T2 and T3. Urinary cystatin B concentrations at T1 were correlated with SSS (Spearman's ρ = 0.690, P < .001). The increased urinary cystatin B concentrations observed in dogs envenomated by V. berus in comparison to controls may indicate renal tubular injury in these patients.

Authors+Show Affiliations

Faculty of Veterinary Medicine, Department of Companion Animal Clinical Sciences, Norwegian University of Life Sciences, Oslo Norway. Electronic address: hannah.harjen@nmbu.no.Faculty of Veterinary Medicine, Department of Companion Animal Clinical Sciences, Norwegian University of Life Sciences, Oslo Norway.Faculty of Veterinary Medicine, Department of Companion Animal Clinical Sciences, Norwegian University of Life Sciences, Oslo Norway.Faculty of Veterinary Medicine, Department of Companion Animal Clinical Sciences, Norwegian University of Life Sciences, Oslo Norway.IDEXX Laboratories, Inc., Westbrook, ME, USA.IDEXX Laboratories, Inc., Westbrook, ME, USA.IDEXX Laboratories, Inc., Westbrook, ME, USA.IDEXX Laboratories, Inc., Westbrook, ME, USA.Faculty of Veterinary Medicine, Department of Preclinical sciences and Pathology, Norwegian University of Life Sciences, Oslo Norway.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34583053

Citation

Harjen, Hannah J., et al. "Evaluation of Urinary Clusterin and Cystatin B as Biomarkers for Renal Injury in Dogs Envenomated By the European Adder (Vipera Berus)." Topics in Companion Animal Medicine, vol. 46, 2022, p. 100586.
Harjen HJ, Anfinsen KP, Hultman J, et al. Evaluation of Urinary Clusterin and Cystatin B as Biomarkers for Renal Injury in Dogs Envenomated by the European Adder (Vipera berus). Top Companion Anim Med. 2022;46:100586.
Harjen, H. J., Anfinsen, K. P., Hultman, J., Moldal, E. R., Szlosek, D., Murphy, R., Friis, H., Peterson, S., & Rørtveit, R. (2022). Evaluation of Urinary Clusterin and Cystatin B as Biomarkers for Renal Injury in Dogs Envenomated by the European Adder (Vipera berus). Topics in Companion Animal Medicine, 46, 100586. https://doi.org/10.1016/j.tcam.2021.100586
Harjen HJ, et al. Evaluation of Urinary Clusterin and Cystatin B as Biomarkers for Renal Injury in Dogs Envenomated By the European Adder (Vipera Berus). Top Companion Anim Med. 2022 Jan-Feb;46:100586. PubMed PMID: 34583053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of Urinary Clusterin and Cystatin B as Biomarkers for Renal Injury in Dogs Envenomated by the European Adder (Vipera berus). AU - Harjen,Hannah J, AU - Anfinsen,Kristin P, AU - Hultman,Josefin, AU - Moldal,Elena R, AU - Szlosek,Donald, AU - Murphy,Rachel, AU - Friis,Hanne, AU - Peterson,Sarah, AU - Rørtveit,Runa, Y1 - 2021/09/25/ PY - 2021/03/23/received PY - 2021/09/04/revised PY - 2021/09/23/accepted PY - 2021/9/29/pubmed PY - 2022/1/27/medline PY - 2021/9/28/entrez KW - acute kidney injury KW - adder KW - biomarker KW - clusterin KW - cystatin B KW - snakebite KW - viper SP - 100586 EP - 100586 JF - Topics in companion animal medicine JO - Top Companion Anim Med VL - 46 N2 - Dogs are commonly bitten by the European adder (Vipera berus) but studies investigating the effects of envenomation are limited. Snakebite-related kidney injury is reported in dogs but diagnosis of acute kidney injury (AKI) might be limited by the insensitivity of routinely used renal function biomarkers. The aim of this study was to evaluate novel biomarkers of renal injury (urinary cystatin B and urinary clusterin) and biomarkers of renal function (serum creatinine and serum symmetric dimethylarginine), and urine protein to creatinine ratio in dogs envenomated by V. berus. Biomarkers were measured at presentation (T1), 12 hours (T2), 24 hours (T3), 36 hours (T4), and 14 days (T5) after snakebite and compared to a group of healthy control dogs. A secondary aim was to investigate the association between biomarker concentrations and severity of clinical signs of envenomation using a snakebite severity score (SSS). Urinary cystatin B concentrations were significantly higher at all timepoints in envenomated dogs compared to controls (P < .010), except for T5 (P = .222). Absolute urinary clusterin concentrations were not significantly different to controls at any timepoint. Compared to controls, serum creatinine and serum symmetric dimethylarginine concentrations were significantly lower in envenomated dogs at T1-T4 (P < .036) and T2-T4 (P < .036), respectively. Urine protein to creatinine ratio was higher in envenomated dogs compared to controls at T2 and T3. Urinary cystatin B concentrations at T1 were correlated with SSS (Spearman's ρ = 0.690, P < .001). The increased urinary cystatin B concentrations observed in dogs envenomated by V. berus in comparison to controls may indicate renal tubular injury in these patients. SN - 1946-9837 UR - https://www.unboundmedicine.com/medline/citation/34583053/Evaluation_of_Urinary_Clusterin_and_Cystatin_B_as_Biomarkers_for_Renal_Injury_in_Dogs_Envenomated_by_the_European_Adder__Vipera_berus__ DB - PRIME DP - Unbound Medicine ER -