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Antioxidant compounds of Kielmeyera coriacea Mart. with α-amylase, lipase and advanced glycation end-product inhibitory activities.
J Pharm Biomed Anal. 2021 Nov 30; 206:114387.JP

Abstract

Chronic hyperglycemia and hyperlipidemia are associated with excessive formation of reactive oxygen species and advanced glycation end-products. The present study aimed to evaluate the potential in vitro antidiabetic properties of Kielmeyera coriacea inner bark. The main phytochemical compounds were identified by UHPLC-ESI/MSn and the ethanol extract and its fractions were used to evaluate their antioxidant and anti-glycation capacities, as well as their inhibitory potential against glycoside and lipid hydrolases activities. The polar fractions, especially the n-butanol fraction, had free radical scavenging and quenching properties (ORAC and FRAP values>1800 and 1000 µmol trolox eq/g, respectively, and DPPH IC50<4 µg/mL), and inhibited ROS production (p < 0.01), lipid peroxidation (p < 0.001), glycation (IC50 ~ 10 µg/mL in the BSA-fructose assay; IC50 ~ 200 µg/mL in the BSA-methylglyoxal and arginine-methylglyoxal assays), α-amylase (IC50<0.1 µg/mL) and lipase (IC50<5 µg/mL), with no cytotoxicity. Biomolecules well-known as potent antioxidants were identified for the first time in the inner bark of K. coriacea, such as protocatechuic acid, epicatechin and procyanidins A, B and C. Together, our results support the antioxidant, anti-glycation and glycoside and lipid hydrolases inhibitory properties of the inner bark of K. coriacea, a species found in the Brazilian savanna, which makes it especially useful to combat oxidative stress and hyperglycemia and hyperlipidemia.

Authors+Show Affiliations

Institute of Biotechnology - Federal University of Uberlândia, Av. Pará, 1720, 38400-902, Uberlândia, MG, Brazil.Institute of Chemistry - Federal University of Uberlândia, Av. João Naves de Ávila, 2121, 38408-100, Uberlândia, MG, Brazil.Institute of Biotechnology - Federal University of Uberlândia, Av. Pará, 1720, 38400-902, Uberlândia, MG, Brazil.Institute of Biotechnology - Federal University of Uberlândia, Av. Pará, 1720, 38400-902, Uberlândia, MG, Brazil.Institute of Biotechnology - Federal University of Uberlândia, Av. Pará, 1720, 38400-902, Uberlândia, MG, Brazil.Institute of Biotechnology - Federal University of Uberlândia, Av. Pará, 1720, 38400-902, Uberlândia, MG, Brazil.Institute of Biotechnology - Federal University of Uberlândia, Av. Pará, 1720, 38400-902, Uberlândia, MG, Brazil.Institute of Biotechnology - Federal University of Uberlândia, Av. Pará, 1720, 38400-902, Uberlândia, MG, Brazil.Institute of Chemistry - Federal University of Uberlândia, Av. João Naves de Ávila, 2121, 38408-100, Uberlândia, MG, Brazil.Institute of Chemistry - Federal University of Uberlândia, Av. João Naves de Ávila, 2121, 38408-100, Uberlândia, MG, Brazil.Institute of Biotechnology - Federal University of Uberlândia, Av. Pará, 1720, 38400-902, Uberlândia, MG, Brazil.Institute of Chemistry - Federal University of Uberlândia, Av. João Naves de Ávila, 2121, 38408-100, Uberlândia, MG, Brazil.Institute of Biotechnology - Federal University of Uberlândia, Av. Pará, 1720, 38400-902, Uberlândia, MG, Brazil. Electronic address: foued@ufu.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34583125

Citation

Justino, Allisson Benatti, et al. "Antioxidant Compounds of Kielmeyera Coriacea Mart. With Α-amylase, Lipase and Advanced Glycation End-product Inhibitory Activities." Journal of Pharmaceutical and Biomedical Analysis, vol. 206, 2021, p. 114387.
Justino AB, Santana EC, Franco RR, et al. Antioxidant compounds of Kielmeyera coriacea Mart. with α-amylase, lipase and advanced glycation end-product inhibitory activities. J Pharm Biomed Anal. 2021;206:114387.
Justino, A. B., Santana, E. C., Franco, R. R., Queiroz, J. S., Silva, H. C. G., de Lima, J. P., Saraiva, A. L., Martins, M. M., Lemos de Morais, S. A., de Oliveira, A., Filho, L. R. G., Aquino, F. J. T., & Espindola, F. S. (2021). Antioxidant compounds of Kielmeyera coriacea Mart. with α-amylase, lipase and advanced glycation end-product inhibitory activities. Journal of Pharmaceutical and Biomedical Analysis, 206, 114387. https://doi.org/10.1016/j.jpba.2021.114387
Justino AB, et al. Antioxidant Compounds of Kielmeyera Coriacea Mart. With Α-amylase, Lipase and Advanced Glycation End-product Inhibitory Activities. J Pharm Biomed Anal. 2021 Nov 30;206:114387. PubMed PMID: 34583125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant compounds of Kielmeyera coriacea Mart. with α-amylase, lipase and advanced glycation end-product inhibitory activities. AU - Justino,Allisson Benatti, AU - Santana,Eder C, AU - Franco,Rodrigo Rodrigues, AU - Queiroz,Julia Silveira, AU - Silva,Heitor Cappato Guerra, AU - de Lima,Joed Pires,Júnior AU - Saraiva,André Lopes, AU - Martins,Mário Machado, AU - Lemos de Morais,Sérgio Antônio, AU - de Oliveira,Alberto, AU - Filho,Luiz Ricardo Goulart, AU - Aquino,Francisco José Torres, AU - Espindola,Foued Salmen, Y1 - 2021/09/20/ PY - 2020/12/09/received PY - 2021/09/01/revised PY - 2021/09/16/accepted PY - 2021/9/29/pubmed PY - 2021/10/14/medline PY - 2021/9/28/entrez KW - Antioxidants KW - Diabetes KW - Enzyme inhibition KW - Glycation KW - Polyphenols SP - 114387 EP - 114387 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 206 N2 - Chronic hyperglycemia and hyperlipidemia are associated with excessive formation of reactive oxygen species and advanced glycation end-products. The present study aimed to evaluate the potential in vitro antidiabetic properties of Kielmeyera coriacea inner bark. The main phytochemical compounds were identified by UHPLC-ESI/MSn and the ethanol extract and its fractions were used to evaluate their antioxidant and anti-glycation capacities, as well as their inhibitory potential against glycoside and lipid hydrolases activities. The polar fractions, especially the n-butanol fraction, had free radical scavenging and quenching properties (ORAC and FRAP values>1800 and 1000 µmol trolox eq/g, respectively, and DPPH IC50<4 µg/mL), and inhibited ROS production (p < 0.01), lipid peroxidation (p < 0.001), glycation (IC50 ~ 10 µg/mL in the BSA-fructose assay; IC50 ~ 200 µg/mL in the BSA-methylglyoxal and arginine-methylglyoxal assays), α-amylase (IC50<0.1 µg/mL) and lipase (IC50<5 µg/mL), with no cytotoxicity. Biomolecules well-known as potent antioxidants were identified for the first time in the inner bark of K. coriacea, such as protocatechuic acid, epicatechin and procyanidins A, B and C. Together, our results support the antioxidant, anti-glycation and glycoside and lipid hydrolases inhibitory properties of the inner bark of K. coriacea, a species found in the Brazilian savanna, which makes it especially useful to combat oxidative stress and hyperglycemia and hyperlipidemia. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/34583125/Antioxidant_compounds_of_Kielmeyera_coriacea_Mart__with_α_amylase_lipase_and_advanced_glycation_end_product_inhibitory_activities_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(21)00498-2 DB - PRIME DP - Unbound Medicine ER -