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Biosynthesis, Characterization and Antibacterial Application of Novel Silver Nanoparticles against Drug Resistant Pathogenic Klebsiella pneumoniae and Salmonella Enteritidis.
Molecules. 2021 Oct 02; 26(19)M

Abstract

The present study highlights the biosynthesis of silver nanoparticles (AgNPs) using culture supernatant of Massilia sp. MAHUQ-52 as well as the antimicrobial application of synthesized AgNPs against multi-drug resistant pathogenic Klebsiella pneumoniae and Salmonella Enteritidis. Well-defined AgNPs formation occurred from the reaction mixture of cell-free supernatant and silver nitrate (AgNO3) solution within 48 h of incubation. UV-visible spectroscopy analysis showed a strong peak at 435 nm, which corresponds to the surface plasmon resonance of AgNPs. The synthesized AgNPs were characterized by FE-TEM, EDX, XRD, DLS and FT-IR. From FE-TEM analysis, it was found that most of the particles were spherical shape, and the size of synthesized nanoparticles (NPs) was 15-55 nm. EDX spectrum revealed a strong silver signal at 3 keV. XRD analysis determined the crystalline, pure, face-centered cubic AgNPs. FT-IR analysis identified various functional molecules that may be involved with the synthesis and stabilization of AgNPs. The antimicrobial activity of Massilia sp. MAHUQ-52 mediated synthesized AgNPs was determined using the disk diffusion method against K. pneumoniae and S. Enteritidis. Biosynthesized AgNPs showed strong antimicrobial activity against both K. pneumoniae and S. Enteritidis. The MICs of synthesized AgNPs against K. pneumoniae and S. Enteritidis were 12.5 and 25.0 μg/mL, respectively. The MBC of biosynthesized AgNPs against both pathogens was 50.0 μg/mL. From FE-SEM analysis, it was found that the AgNPs-treated cells showed morphological changes with irregular and damaged cell walls that culminated in cell death.

Authors+Show Affiliations

Department of Food and Nutrition, College of Biotechnology and Natural Resource, Chung-Ang University, Anseong 17546, Korea.Department of Food Science and Biotechnology, Gachon University, Seongnam 461-701, Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34641540

Citation

Huq, Md Amdadul, and Shahina Akter. "Biosynthesis, Characterization and Antibacterial Application of Novel Silver Nanoparticles Against Drug Resistant Pathogenic Klebsiella Pneumoniae and Salmonella Enteritidis." Molecules (Basel, Switzerland), vol. 26, no. 19, 2021.
Huq MA, Akter S. Biosynthesis, Characterization and Antibacterial Application of Novel Silver Nanoparticles against Drug Resistant Pathogenic Klebsiella pneumoniae and Salmonella Enteritidis. Molecules. 2021;26(19).
Huq, M. A., & Akter, S. (2021). Biosynthesis, Characterization and Antibacterial Application of Novel Silver Nanoparticles against Drug Resistant Pathogenic Klebsiella pneumoniae and Salmonella Enteritidis. Molecules (Basel, Switzerland), 26(19). https://doi.org/10.3390/molecules26195996
Huq MA, Akter S. Biosynthesis, Characterization and Antibacterial Application of Novel Silver Nanoparticles Against Drug Resistant Pathogenic Klebsiella Pneumoniae and Salmonella Enteritidis. Molecules. 2021 Oct 2;26(19) PubMed PMID: 34641540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biosynthesis, Characterization and Antibacterial Application of Novel Silver Nanoparticles against Drug Resistant Pathogenic Klebsiella pneumoniae and Salmonella Enteritidis. AU - Huq,Md Amdadul, AU - Akter,Shahina, Y1 - 2021/10/02/ PY - 2021/08/21/received PY - 2021/09/27/revised PY - 2021/09/27/accepted PY - 2021/10/13/entrez PY - 2021/10/14/pubmed PY - 2021/11/19/medline KW - AgNPs KW - K. pneumoniae KW - Massilia sp. MAHUQ-52 KW - S. Enteritidis KW - biosynthesis JF - Molecules (Basel, Switzerland) JO - Molecules VL - 26 IS - 19 N2 - The present study highlights the biosynthesis of silver nanoparticles (AgNPs) using culture supernatant of Massilia sp. MAHUQ-52 as well as the antimicrobial application of synthesized AgNPs against multi-drug resistant pathogenic Klebsiella pneumoniae and Salmonella Enteritidis. Well-defined AgNPs formation occurred from the reaction mixture of cell-free supernatant and silver nitrate (AgNO3) solution within 48 h of incubation. UV-visible spectroscopy analysis showed a strong peak at 435 nm, which corresponds to the surface plasmon resonance of AgNPs. The synthesized AgNPs were characterized by FE-TEM, EDX, XRD, DLS and FT-IR. From FE-TEM analysis, it was found that most of the particles were spherical shape, and the size of synthesized nanoparticles (NPs) was 15-55 nm. EDX spectrum revealed a strong silver signal at 3 keV. XRD analysis determined the crystalline, pure, face-centered cubic AgNPs. FT-IR analysis identified various functional molecules that may be involved with the synthesis and stabilization of AgNPs. The antimicrobial activity of Massilia sp. MAHUQ-52 mediated synthesized AgNPs was determined using the disk diffusion method against K. pneumoniae and S. Enteritidis. Biosynthesized AgNPs showed strong antimicrobial activity against both K. pneumoniae and S. Enteritidis. The MICs of synthesized AgNPs against K. pneumoniae and S. Enteritidis were 12.5 and 25.0 μg/mL, respectively. The MBC of biosynthesized AgNPs against both pathogens was 50.0 μg/mL. From FE-SEM analysis, it was found that the AgNPs-treated cells showed morphological changes with irregular and damaged cell walls that culminated in cell death. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/34641540/Biosynthesis_Characterization_and_Antibacterial_Application_of_Novel_Silver_Nanoparticles_against_Drug_Resistant_Pathogenic_Klebsiella_pneumoniae_and_Salmonella_Enteritidis_ L2 - https://www.mdpi.com/resolver?pii=molecules26195996 DB - PRIME DP - Unbound Medicine ER -