Tags

Type your tag names separated by a space and hit enter

Identification and Evaluation of Wheat-Aegilops bicornis Lines with Resistance to Powdery Mildew and Stripe Rust.
Plant Dis. 2022 Mar; 106(3):864-871.PD

Abstract

Wheat pathogens, especially those causing powdery mildew and stripe rust, seriously threaten yield worldwide. Utilizing newly identified disease resistance genes from wheat relatives is an effective strategy to minimize disease damage. In this study, chromosome-specific molecular markers for the 3Sb and 7Sb chromosomes of Aegilops bicornis were developed using PCR-based landmark unique gene primers for screening wheat-A. bicornis progenies. Fluorescence in situ hybridization (FISH) was performed to further identify wheat-A. bicornis progenies using oligonucleotides probes Oligo-pSc119.2-1, Oligo-pTa535-1, and Oligo-(GAA)8. After establishing A. bicornis 3Sb and 7Sb chromosome-specific FISH markers, Holdfast (common wheat)-A. bicornis 3Sb addition, 7Sb addition, 3Sb(3A) substitution, 3Sb(3B) substitution, 3Sb(3D) substitution, 7Sb(7A) substitution, and 7Sb(7B) substitution lines were identified by the molecular and cytological markers. Stripe rust and powdery mildew resistance, along with agronomic traits, were investigated to evaluate the breeding potential of these lines. Holdfast and Holdfast-A. bicornis progenies were all highly resistant to stripe rust, indicating that the stripe rust resistance might derive from Holdfast. However, Holdfast-A. bicornis 3Sb addition, 3Sb(3A) substitution, 3Sb(3B) substitution, and 3Sb(3D) substitution lines showed high resistance to powdery mildew while Holdfast was highly susceptible, indicating that chromosome 3Sb of A. bicornis carries previously unknown powdery mildew resistance gene(s). Additionally, the transfer of the 3Sb chromosome from A. bicornis to wheat significantly increased tiller number, but chromosome 7Sb has a negative effect on agronomic traits. Therefore, wheat germplasm containing A. bicornis chromosome 3Sb has potential to contribute to improving powdery mildew resistance and tiller number during wheat breeding.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Wang X
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Han R
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Chen Z
College of Agricultural, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
Li J
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Zhu T
College of Life Science, Yantai University, Yantai, Shandong 264005, China.
Guo J
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Xu W
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Zi Y
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Li F
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Zhai S
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Li H
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Liu J
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Liu A
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Cheng D
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Song J
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
Jia J
College of Agricultural, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
Ma P
College of Life Science, Yantai University, Yantai, Shandong 264005, China.
Liu C
Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.

MeSH

AegilopsChromosomes, PlantIn Situ Hybridization, FluorescencePlant BreedingPlant DiseasesTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34645309

Citation

Wang, Xiaolu, et al. "Identification and Evaluation of Wheat-Aegilops Bicornis Lines With Resistance to Powdery Mildew and Stripe Rust." Plant Disease, vol. 106, no. 3, 2022, pp. 864-871.
Wang X, Han R, Chen Z, et al. Identification and Evaluation of Wheat-Aegilops bicornis Lines with Resistance to Powdery Mildew and Stripe Rust. Plant Dis. 2022;106(3):864-871.
Wang, X., Han, R., Chen, Z., Li, J., Zhu, T., Guo, J., Xu, W., Zi, Y., Li, F., Zhai, S., Li, H., Liu, J., Liu, A., Cheng, D., Song, J., Jia, J., Ma, P., & Liu, C. (2022). Identification and Evaluation of Wheat-Aegilops bicornis Lines with Resistance to Powdery Mildew and Stripe Rust. Plant Disease, 106(3), 864-871. https://doi.org/10.1094/PDIS-05-21-0982-RE
Wang X, et al. Identification and Evaluation of Wheat-Aegilops Bicornis Lines With Resistance to Powdery Mildew and Stripe Rust. Plant Dis. 2022;106(3):864-871. PubMed PMID: 34645309.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and Evaluation of Wheat-Aegilops bicornis Lines with Resistance to Powdery Mildew and Stripe Rust. AU - Wang,Xiaolu, AU - Han,Ran, AU - Chen,Zhiwei, AU - Li,Jianbo, AU - Zhu,Tong, AU - Guo,Jun, AU - Xu,Wenjing, AU - Zi,Yan, AU - Li,Faji, AU - Zhai,Shengnan, AU - Li,Haosheng, AU - Liu,Jianjun, AU - Liu,Aifeng, AU - Cheng,Dungong, AU - Song,Jianmin, AU - Jia,Juqing, AU - Ma,Pengtao, AU - Liu,Cheng, Y1 - 2022/03/10/ PY - 2021/10/15/pubmed PY - 2022/4/2/medline PY - 2021/10/14/entrez KW - Aegilops bicornis KW - FISH KW - agronomic traits KW - disease resistance KW - molecular marker SP - 864 EP - 871 JF - Plant disease JO - Plant Dis VL - 106 IS - 3 N2 - Wheat pathogens, especially those causing powdery mildew and stripe rust, seriously threaten yield worldwide. Utilizing newly identified disease resistance genes from wheat relatives is an effective strategy to minimize disease damage. In this study, chromosome-specific molecular markers for the 3Sb and 7Sb chromosomes of Aegilops bicornis were developed using PCR-based landmark unique gene primers for screening wheat-A. bicornis progenies. Fluorescence in situ hybridization (FISH) was performed to further identify wheat-A. bicornis progenies using oligonucleotides probes Oligo-pSc119.2-1, Oligo-pTa535-1, and Oligo-(GAA)8. After establishing A. bicornis 3Sb and 7Sb chromosome-specific FISH markers, Holdfast (common wheat)-A. bicornis 3Sb addition, 7Sb addition, 3Sb(3A) substitution, 3Sb(3B) substitution, 3Sb(3D) substitution, 7Sb(7A) substitution, and 7Sb(7B) substitution lines were identified by the molecular and cytological markers. Stripe rust and powdery mildew resistance, along with agronomic traits, were investigated to evaluate the breeding potential of these lines. Holdfast and Holdfast-A. bicornis progenies were all highly resistant to stripe rust, indicating that the stripe rust resistance might derive from Holdfast. However, Holdfast-A. bicornis 3Sb addition, 3Sb(3A) substitution, 3Sb(3B) substitution, and 3Sb(3D) substitution lines showed high resistance to powdery mildew while Holdfast was highly susceptible, indicating that chromosome 3Sb of A. bicornis carries previously unknown powdery mildew resistance gene(s). Additionally, the transfer of the 3Sb chromosome from A. bicornis to wheat significantly increased tiller number, but chromosome 7Sb has a negative effect on agronomic traits. Therefore, wheat germplasm containing A. bicornis chromosome 3Sb has potential to contribute to improving powdery mildew resistance and tiller number during wheat breeding. SN - 0191-2917 UR - https://www.unboundmedicine.com/medline/citation/34645309/Identification_and_Evaluation_of_Wheat_Aegilops_bicornis_Lines_with_Resistance_to_Powdery_Mildew_and_Stripe_Rust_ DB - PRIME DP - Unbound Medicine ER -

Related Citations

More