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Genetic dissection of quantitative trait loci for grain size and weight by high-resolution genetic mapping in bread wheat (Triticum aestivum L.).
Theor Appl Genet. 2022 Jan; 135(1):257-271.TA

Abstract

KEY MESSAGE

Six major QTLs for wheat grain size and weight were identified on chromosomes 4A, 4B, 5A and 6A across multiple environments, and were validated in different genetic backgrounds. Grain size and weight are crucial components of wheat yield. Dissection of their genetic control is thus essential for the improvement of yield potential in wheat breeding. We used a doubled haploid (DH) population to detect quantitative trait loci (QTLs) for grain width (GW), grain length (GL), and thousand grain weight (TGW) in five environments. Six major QTLs, QGw.cib-4B.2, QGl.cib-4A, QGl.cib-5A.1, QGl.cib-6A, QTgw.cib-4B, and QTgw.cib-5A, were consistently identified in at least three individual environments and in best linear unbiased prediction (BLUP) datasets, and explained 5.65-34.06% of phenotypic variation. QGw.cib-4B.2, QTgw.cib-4B, QGl.cib-5A.1 and QGl.cib-6A had no effect on grain number per spike (GNS). In addition to QGl.cib-4A, the other major QTLs were further validated by using Kompetitive Allele Specific PCR (KASP) markers in different genetic backgrounds. Moreover, significant interactions between the three major GL QTLs and two major TGW QTLs were observed. Comparison analysis showed that QGl.cib-5A.1 and QGl.cib-6A are likely new loci. Notably, QGw.cib-4B.2 and QTgw.cib-4B were co-located on chromosome 4B and improved TGW by increasing only GW, unlike nearby or overlapped loci reported previously. Three genes associated with grain development within the QGw.cib-4B.2/QTgw.cib-4B interval were identified by searches on sequence similarity, spatial expression patterns, and orthologs. The major QTLs and KASP markers reported here will be useful for elucidating the genetic architecture of grain size and weight and for developing new wheat cultivars with high and stable yield.

Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, China. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, 611130, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, China. ymwei@sicau.edu.cn. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, 611130, China. ymwei@sicau.edu.cn.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China. hailong@cib.ac.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34647130

Citation

Li, Tao, et al. "Genetic Dissection of Quantitative Trait Loci for Grain Size and Weight By High-resolution Genetic Mapping in Bread Wheat (Triticum Aestivum L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 135, no. 1, 2022, pp. 257-271.
Li T, Deng G, Su Y, et al. Genetic dissection of quantitative trait loci for grain size and weight by high-resolution genetic mapping in bread wheat (Triticum aestivum L.). Theor Appl Genet. 2022;135(1):257-271.
Li, T., Deng, G., Su, Y., Yang, Z., Tang, Y., Wang, J., Zhang, J., Qiu, X., Pu, X., Yang, W., Li, J., Liu, Z., Zhang, H., Liang, J., Yu, M., Wei, Y., & Long, H. (2022). Genetic dissection of quantitative trait loci for grain size and weight by high-resolution genetic mapping in bread wheat (Triticum aestivum L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 135(1), 257-271. https://doi.org/10.1007/s00122-021-03964-2
Li T, et al. Genetic Dissection of Quantitative Trait Loci for Grain Size and Weight By High-resolution Genetic Mapping in Bread Wheat (Triticum Aestivum L.). Theor Appl Genet. 2022;135(1):257-271. PubMed PMID: 34647130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic dissection of quantitative trait loci for grain size and weight by high-resolution genetic mapping in bread wheat (Triticum aestivum L.). AU - Li,Tao, AU - Deng,Guangbing, AU - Su,Yan, AU - Yang,Zhao, AU - Tang,Yanyan, AU - Wang,Jinhui, AU - Zhang,Juanyu, AU - Qiu,Xvebing, AU - Pu,Xi, AU - Yang,Wuyun, AU - Li,Jun, AU - Liu,Zehou, AU - Zhang,Haili, AU - Liang,Junjun, AU - Yu,Maoqun, AU - Wei,Yuming, AU - Long,Hai, Y1 - 2021/10/13/ PY - 2021/06/29/received PY - 2021/09/29/accepted PY - 2021/10/15/pubmed PY - 2022/3/18/medline PY - 2021/10/14/entrez SP - 257 EP - 271 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 135 IS - 1 N2 - KEY MESSAGE: Six major QTLs for wheat grain size and weight were identified on chromosomes 4A, 4B, 5A and 6A across multiple environments, and were validated in different genetic backgrounds. Grain size and weight are crucial components of wheat yield. Dissection of their genetic control is thus essential for the improvement of yield potential in wheat breeding. We used a doubled haploid (DH) population to detect quantitative trait loci (QTLs) for grain width (GW), grain length (GL), and thousand grain weight (TGW) in five environments. Six major QTLs, QGw.cib-4B.2, QGl.cib-4A, QGl.cib-5A.1, QGl.cib-6A, QTgw.cib-4B, and QTgw.cib-5A, were consistently identified in at least three individual environments and in best linear unbiased prediction (BLUP) datasets, and explained 5.65-34.06% of phenotypic variation. QGw.cib-4B.2, QTgw.cib-4B, QGl.cib-5A.1 and QGl.cib-6A had no effect on grain number per spike (GNS). In addition to QGl.cib-4A, the other major QTLs were further validated by using Kompetitive Allele Specific PCR (KASP) markers in different genetic backgrounds. Moreover, significant interactions between the three major GL QTLs and two major TGW QTLs were observed. Comparison analysis showed that QGl.cib-5A.1 and QGl.cib-6A are likely new loci. Notably, QGw.cib-4B.2 and QTgw.cib-4B were co-located on chromosome 4B and improved TGW by increasing only GW, unlike nearby or overlapped loci reported previously. Three genes associated with grain development within the QGw.cib-4B.2/QTgw.cib-4B interval were identified by searches on sequence similarity, spatial expression patterns, and orthologs. The major QTLs and KASP markers reported here will be useful for elucidating the genetic architecture of grain size and weight and for developing new wheat cultivars with high and stable yield. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/34647130/Genetic_dissection_of_quantitative_trait_loci_for_grain_size_and_weight_by_high_resolution_genetic_mapping_in_bread_wheat__Triticum_aestivum_L___ L2 - https://dx.doi.org/10.1007/s00122-021-03964-2 DB - PRIME DP - Unbound Medicine ER -