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Molecular Cytogenetic Analysis of the Introgression between Agropyron cristatum P Genome and Wheat Genome.
Int J Mol Sci. 2021 Oct 18; 22(20)IJ

Abstract

Agropyron cristatum (2n = 4x = 28, PPPP) is an important wild relative of common wheat (Triticum aestivum L., 2n = 6x = 42). A previous report showed that the wheat-A. cristatum 6P translocation line WAT655 carrying A. cristatum 6PS (0.81-1.00) exhibited high resistance to prevalent physiological races of stripe rust (CYR32 and CYR33). In this study, three disease resistance-related transcripts, which were mapped to A. cristatum 6PS (0.81-1.00) through the analysis of specific molecular markers, were acquired from among A. cristatum full-length transcripts. The BC5F2 and BC5F2:3 genetic populations of the translocation line WAT655 were analyzed by using three disease resistance-related gene markers, A. cristatum P genome-specific markers, and fluorescence in situ hybridization (FISH). The results revealed that the introgression between A. cristatum P genome and wheat genome was observed in progenies of the genetic populations of the translocation line WAT655 and the physical positions of the three genes were considerably adjacent on A. cristatum 6PS (0.81-1.00) according to the FISH results. Additionally, kompetitive allele-specific PCR (KASP) markers of the three genes were developed to detect and acquire 24 breeding lines selected from the progenies of the distant hybridization of wheat and A. cristatum, which showed resistance to physiological races of stripe rust (CYR32 and CYR33) and other desirable agronomic traits according to the field investigation. In conclusion, this study not only provides new insights into the introgression between A. cristatum P genome and wheat genome but also provides the desirable germplasms for breeding practice.

Authors+Show Affiliations

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology of Sciences, Shijiazhuang 050022, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34681868

Citation

Zhang, Zhi, et al. "Molecular Cytogenetic Analysis of the Introgression Between Agropyron Cristatum P Genome and Wheat Genome." International Journal of Molecular Sciences, vol. 22, no. 20, 2021.
Zhang Z, Zhou S, Liu W, et al. Molecular Cytogenetic Analysis of the Introgression between Agropyron cristatum P Genome and Wheat Genome. Int J Mol Sci. 2021;22(20).
Zhang, Z., Zhou, S., Liu, W., Song, L., Zhang, J., Han, H., Yang, X., Lin, Y., Li, X., & Li, L. (2021). Molecular Cytogenetic Analysis of the Introgression between Agropyron cristatum P Genome and Wheat Genome. International Journal of Molecular Sciences, 22(20). https://doi.org/10.3390/ijms222011208
Zhang Z, et al. Molecular Cytogenetic Analysis of the Introgression Between Agropyron Cristatum P Genome and Wheat Genome. Int J Mol Sci. 2021 Oct 18;22(20) PubMed PMID: 34681868.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular Cytogenetic Analysis of the Introgression between Agropyron cristatum P Genome and Wheat Genome. AU - Zhang,Zhi, AU - Zhou,Shenghui, AU - Liu,Weihua, AU - Song,Liqiang, AU - Zhang,Jinpeng, AU - Han,Haiming, AU - Yang,Xinming, AU - Lin,Yida, AU - Li,Xiuquan, AU - Li,Lihui, Y1 - 2021/10/18/ PY - 2021/09/21/received PY - 2021/10/14/revised PY - 2021/10/15/accepted PY - 2021/10/23/entrez PY - 2021/10/24/pubmed PY - 2021/12/17/medline KW - Agropyron cristatum KW - common wheat KW - introgression KW - stripe rust KW - translocation line JF - International journal of molecular sciences JO - Int J Mol Sci VL - 22 IS - 20 N2 - Agropyron cristatum (2n = 4x = 28, PPPP) is an important wild relative of common wheat (Triticum aestivum L., 2n = 6x = 42). A previous report showed that the wheat-A. cristatum 6P translocation line WAT655 carrying A. cristatum 6PS (0.81-1.00) exhibited high resistance to prevalent physiological races of stripe rust (CYR32 and CYR33). In this study, three disease resistance-related transcripts, which were mapped to A. cristatum 6PS (0.81-1.00) through the analysis of specific molecular markers, were acquired from among A. cristatum full-length transcripts. The BC5F2 and BC5F2:3 genetic populations of the translocation line WAT655 were analyzed by using three disease resistance-related gene markers, A. cristatum P genome-specific markers, and fluorescence in situ hybridization (FISH). The results revealed that the introgression between A. cristatum P genome and wheat genome was observed in progenies of the genetic populations of the translocation line WAT655 and the physical positions of the three genes were considerably adjacent on A. cristatum 6PS (0.81-1.00) according to the FISH results. Additionally, kompetitive allele-specific PCR (KASP) markers of the three genes were developed to detect and acquire 24 breeding lines selected from the progenies of the distant hybridization of wheat and A. cristatum, which showed resistance to physiological races of stripe rust (CYR32 and CYR33) and other desirable agronomic traits according to the field investigation. In conclusion, this study not only provides new insights into the introgression between A. cristatum P genome and wheat genome but also provides the desirable germplasms for breeding practice. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/34681868/Molecular_Cytogenetic_Analysis_of_the_Introgression_between_Agropyron_cristatum_P_Genome_and_Wheat_Genome_ L2 - https://www.mdpi.com/resolver?pii=ijms222011208 DB - PRIME DP - Unbound Medicine ER -