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[Effect of electroacupuncture combined with Zhuang-medicine-thread moxibustion on silent information regulator-1/nuclear factor κB signaling pathway in gastric antrum of diabetic gastroparesis rats].
Zhen Ci Yan Jiu. 2021 Oct 25; 46(10):837-44.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) combined with Zhuang-medicine-thread moxibustion on silent information regulator-1 (SIRT1)/nuclear factor kappa B (NF-κB) signal pathway and inflammatory factor expression in gastric antrum tissue of diabetic gastroparesis (DGP) rats, so as to explore its mechanism underlying improvement of DGP.

METHODS

Male SD rats were randomly divided into normal, model, medication, EA, Zhuang-medicine-thread moxibustion (moxibustion) and EA+moxibustion groups (n=12 per group). The DGP model was established by intraperitoneal injection of streptozotocin (STZ). Rats of the medication group were treated by gavage of 0.15 mg/mL mosapride citrate suspension. EA (10 Hz /50 Hz, 2 mA) or moxibustion (3 cones) or EA+moxibustion was applied to "Zhongwan"(CV12), bilateral "Neiguan"(PC6) and bilateral "Sanyinjiao"(SP6) of the related group for 20 min, once a day for 3 weeks. Blood glucose, gastric emptying rate and intestinal propulsion rate were measured. The levels of serum interleukin (IL)-6, IL-8, IL-10 and tumor necrosis factor-α(TNF-α) were detected by ELISA; the phosphorylation level of the phosphorylated inhibitor of nuclear factor κBα inhibitor (pIκ-Bα), the protein and mRNA expression of NF-κB p65 and SIRT1 in the gastric antrum tissue were detected by Western blot and real-time quantifitative PCR, respectively.

RESULTS

(1) Compared with the normal group, the levels of blood glucose, serum IL-6, IL-8, TNF-α, and gastric pIκ-Bα and NF-κB p65 protein and mRNA expressions were significantly increased (P<0.01), and the gastric emptying rate, intestinal propulsion rate, serum IL-10 level, and SIRT1 protein and mRNA expressions were considerably decreased in the model group (P<0.01). (2) In contrast to the model group, the blood glucose in the EA, moxibustion and EA+moxibustion groups, serum IL-6, IL-8 and TNF-α levels in the 4 treatment groups, as well as NF-κB p65 protein expression in the medication and EA+moxibustion groups, and NF-κB p65 mRNA expression and pIκ-Bα protein and mRNA expression in the 4 treatment groups were significantly decreased (P<0.01, P<0.05); while the gastric emptying rate and intestinal propulsive rate and IL-10 content in the 4 treatment groups, and SIRT1 protein and mRNA expression in the medication and EA+moxibustion groups were obviously increased (P<0.05, P<0.01). (3) The effects of EA+moxibustion were significantly superior to those of simple EA and moxibustion in increasing gastric emptying rate, IL-10, SIRT1 protein expression (P<0.05, P<0.01), and in lowering IL-8 and TNF-α contents, pIκ-Bα protein and mRNA expression and NF-κB p65 mRNA expression (P<0.05, P<0.01). No significant differences were found among the 4 intervention groups in promoting the intestinal propulsive rate and among the EA, moxibustion and EA+moxibustion groups in lowering blood glucose (P>0.05).

CONCLUSION

EA combined with Zhuang-medicine-thread moxibustion can effectively reduce the level of serum inflammatory factors and regulate SIRT1/NF-κB signal pathway in DGP rats, which may contribute to its function in improving gastrointestinal movement.

Authors+Show Affiliations

Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.College of Acupuncture-moxibustion and Tuina, Guangxi University of Chinese Medicine, Nanning 530001, China.Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

34698457

Citation

Mai, Wei, et al. "[Effect of Electroacupuncture Combined With Zhuang-medicine-thread Moxibustion On Silent Information Regulator-1/nuclear Factor κB Signaling Pathway in Gastric Antrum of Diabetic Gastroparesis Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 46, no. 10, 2021, pp. 837-44.
Mai W, Fan YS, Miao FR, et al. [Effect of electroacupuncture combined with Zhuang-medicine-thread moxibustion on silent information regulator-1/nuclear factor κB signaling pathway in gastric antrum of diabetic gastroparesis rats]. Zhen Ci Yan Jiu. 2021;46(10):837-44.
Mai, W., Fan, Y. S., Miao, F. R., He, C., Huang, L. L., Zhao, X. J., Nie, Y. J., & Pang, R. K. (2021). [Effect of electroacupuncture combined with Zhuang-medicine-thread moxibustion on silent information regulator-1/nuclear factor κB signaling pathway in gastric antrum of diabetic gastroparesis rats]. Zhen Ci Yan Jiu = Acupuncture Research, 46(10), 837-44. https://doi.org/10.13702/j.1000-0607.200942
Mai W, et al. [Effect of Electroacupuncture Combined With Zhuang-medicine-thread Moxibustion On Silent Information Regulator-1/nuclear Factor κB Signaling Pathway in Gastric Antrum of Diabetic Gastroparesis Rats]. Zhen Ci Yan Jiu. 2021 Oct 25;46(10):837-44. PubMed PMID: 34698457.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture combined with Zhuang-medicine-thread moxibustion on silent information regulator-1/nuclear factor κB signaling pathway in gastric antrum of diabetic gastroparesis rats]. AU - Mai,Wei, AU - Fan,Yu-Shan, AU - Miao,Fu-Rui, AU - He,Cai, AU - Huang,Li-Lin, AU - Zhao,Xiao-Jun, AU - Nie,Ying-Jie, AU - Pang,Rui-Kang, PY - 2021/10/26/entrez PY - 2021/10/27/pubmed PY - 2021/10/28/medline KW - Diabetic gastroparesis KW - Electroacupuncture KW - Gastric SIRT1/NF-κB signaling KW - Gastrointestinal movement KW - Inflammatory factors KW - Zhuang-medicine-thread moxibustion SP - 837 EP - 44 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 46 IS - 10 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) combined with Zhuang-medicine-thread moxibustion on silent information regulator-1 (SIRT1)/nuclear factor kappa B (NF-κB) signal pathway and inflammatory factor expression in gastric antrum tissue of diabetic gastroparesis (DGP) rats, so as to explore its mechanism underlying improvement of DGP. METHODS: Male SD rats were randomly divided into normal, model, medication, EA, Zhuang-medicine-thread moxibustion (moxibustion) and EA+moxibustion groups (n=12 per group). The DGP model was established by intraperitoneal injection of streptozotocin (STZ). Rats of the medication group were treated by gavage of 0.15 mg/mL mosapride citrate suspension. EA (10 Hz /50 Hz, 2 mA) or moxibustion (3 cones) or EA+moxibustion was applied to "Zhongwan"(CV12), bilateral "Neiguan"(PC6) and bilateral "Sanyinjiao"(SP6) of the related group for 20 min, once a day for 3 weeks. Blood glucose, gastric emptying rate and intestinal propulsion rate were measured. The levels of serum interleukin (IL)-6, IL-8, IL-10 and tumor necrosis factor-α(TNF-α) were detected by ELISA; the phosphorylation level of the phosphorylated inhibitor of nuclear factor κBα inhibitor (pIκ-Bα), the protein and mRNA expression of NF-κB p65 and SIRT1 in the gastric antrum tissue were detected by Western blot and real-time quantifitative PCR, respectively. RESULTS: (1) Compared with the normal group, the levels of blood glucose, serum IL-6, IL-8, TNF-α, and gastric pIκ-Bα and NF-κB p65 protein and mRNA expressions were significantly increased (P<0.01), and the gastric emptying rate, intestinal propulsion rate, serum IL-10 level, and SIRT1 protein and mRNA expressions were considerably decreased in the model group (P<0.01). (2) In contrast to the model group, the blood glucose in the EA, moxibustion and EA+moxibustion groups, serum IL-6, IL-8 and TNF-α levels in the 4 treatment groups, as well as NF-κB p65 protein expression in the medication and EA+moxibustion groups, and NF-κB p65 mRNA expression and pIκ-Bα protein and mRNA expression in the 4 treatment groups were significantly decreased (P<0.01, P<0.05); while the gastric emptying rate and intestinal propulsive rate and IL-10 content in the 4 treatment groups, and SIRT1 protein and mRNA expression in the medication and EA+moxibustion groups were obviously increased (P<0.05, P<0.01). (3) The effects of EA+moxibustion were significantly superior to those of simple EA and moxibustion in increasing gastric emptying rate, IL-10, SIRT1 protein expression (P<0.05, P<0.01), and in lowering IL-8 and TNF-α contents, pIκ-Bα protein and mRNA expression and NF-κB p65 mRNA expression (P<0.05, P<0.01). No significant differences were found among the 4 intervention groups in promoting the intestinal propulsive rate and among the EA, moxibustion and EA+moxibustion groups in lowering blood glucose (P>0.05). CONCLUSION: EA combined with Zhuang-medicine-thread moxibustion can effectively reduce the level of serum inflammatory factors and regulate SIRT1/NF-κB signal pathway in DGP rats, which may contribute to its function in improving gastrointestinal movement. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/34698457/[Effect_of_electroacupuncture_combined_with_Zhuang_medicine_thread_moxibustion_on_silent_information_regulator_1/nuclear_factor_κB_signaling_pathway_in_gastric_antrum_of_diabetic_gastroparesis_rats]_ DB - PRIME DP - Unbound Medicine ER -