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Development and Identification of a Novel Wheat-Thinopyrum scirpeum 4E (4D) Chromosomal Substitution Line with Stripe Rust and Powdery Mildew Resistance.
Plant Dis. 2022 Mar; 106(3):975-983.PD

Abstract

Stripe rust caused by Puccinia striiformis f. sp. tritici and powdery mildew caused by Blumeria graminis f. sp. tritici are devastating diseases of wheat worldwide. Exploration of new disease-resistant genes from cultivated wheat and wild relatives are the most effective means of reducing the amounts of fungicides applied to combat these diseases. Thinopyrum scirpeum (2n = 4x = 28, EEEE) is an important promising reservoir of useful genes, including stripe rust and powdery mildew resistance, and may be useful for increasing wheat disease resistance. Here, we characterize a novel wheat-Th. scirpeum disomic substitution line, K16-730-3, and chromosome-specific markers were developed that can be used to trace the Th. scirpeum chromosome or chromosome segments transferred into wheat. Genomic in situ hybridization and fluorescence in situ hybridization analyses indicated that K16-730-3 is a new 4E (4D) chromosomal substitution line. Evaluation of seedling and adult disease responses revealed that K16-730-3 is resistant to stripe rust and powdery mildew. In addition, no obvious difference in grain yield was observed between K16-730-3 and its wheat parents. Genotyping-by-sequencing analyses indicated that 74 PCR-based markers can accurately trace chromosome 4E, which were linked to the disease resistance genes in the wheat background. Further marker validation analyses revealed that 13 specific markers can distinguish between the E-genome chromosomes of Th. scirpeum and the chromosomes of other wheat-related species. The new substitution line K16-730-3 carrying the stripe rust and powdery mildew resistance genes will be useful as novel germplasm in breeding for disease resistance. The markers developed in this study can be used in marker-assisted selection for increasing disease resistance in wheat.

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Authors+Show Affiliations

Gong B
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Zhang H
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Yang Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Zhang J
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Zhu W
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Xu L
Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Wang Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Zeng J
College of Resources, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Fan X
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Sha L
College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Zhang H
College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Wu D
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Chen G
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Zhou Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Kang H
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.

MeSH

Chromosomes, PlantIn Situ Hybridization, FluorescencePlant BreedingPlant DiseasesTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34698515

Citation

Gong, Biran, et al. "Development and Identification of a Novel Wheat-Thinopyrum Scirpeum 4E (4D) Chromosomal Substitution Line With Stripe Rust and Powdery Mildew Resistance." Plant Disease, vol. 106, no. 3, 2022, pp. 975-983.
Gong B, Zhang H, Yang Y, et al. Development and Identification of a Novel Wheat-Thinopyrum scirpeum 4E (4D) Chromosomal Substitution Line with Stripe Rust and Powdery Mildew Resistance. Plant Dis. 2022;106(3):975-983.
Gong, B., Zhang, H., Yang, Y., Zhang, J., Zhu, W., Xu, L., Wang, Y., Zeng, J., Fan, X., Sha, L., Zhang, H., Wu, D., Chen, G., Zhou, Y., & Kang, H. (2022). Development and Identification of a Novel Wheat-Thinopyrum scirpeum 4E (4D) Chromosomal Substitution Line with Stripe Rust and Powdery Mildew Resistance. Plant Disease, 106(3), 975-983. https://doi.org/10.1094/PDIS-08-21-1599-RE
Gong B, et al. Development and Identification of a Novel Wheat-Thinopyrum Scirpeum 4E (4D) Chromosomal Substitution Line With Stripe Rust and Powdery Mildew Resistance. Plant Dis. 2022;106(3):975-983. PubMed PMID: 34698515.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and Identification of a Novel Wheat-Thinopyrum scirpeum 4E (4D) Chromosomal Substitution Line with Stripe Rust and Powdery Mildew Resistance. AU - Gong,Biran, AU - Zhang,Hao, AU - Yang,Yulu, AU - Zhang,Juwei, AU - Zhu,Wei, AU - Xu,Lili, AU - Wang,Yi, AU - Zeng,Jian, AU - Fan,Xing, AU - Sha,Lina, AU - Zhang,Haiqin, AU - Wu,DanDan, AU - Chen,Guoyue, AU - Zhou,Yonghong, AU - Kang,Houyang, Y1 - 2022/03/06/ PY - 2021/10/27/pubmed PY - 2022/4/2/medline PY - 2021/10/26/entrez KW - Thinopyrum scirpeum KW - chromosomal substitution KW - genotyping by sequencing KW - powdery mildew KW - stripe rust SP - 975 EP - 983 JF - Plant disease JO - Plant Dis VL - 106 IS - 3 N2 - Stripe rust caused by Puccinia striiformis f. sp. tritici and powdery mildew caused by Blumeria graminis f. sp. tritici are devastating diseases of wheat worldwide. Exploration of new disease-resistant genes from cultivated wheat and wild relatives are the most effective means of reducing the amounts of fungicides applied to combat these diseases. Thinopyrum scirpeum (2n = 4x = 28, EEEE) is an important promising reservoir of useful genes, including stripe rust and powdery mildew resistance, and may be useful for increasing wheat disease resistance. Here, we characterize a novel wheat-Th. scirpeum disomic substitution line, K16-730-3, and chromosome-specific markers were developed that can be used to trace the Th. scirpeum chromosome or chromosome segments transferred into wheat. Genomic in situ hybridization and fluorescence in situ hybridization analyses indicated that K16-730-3 is a new 4E (4D) chromosomal substitution line. Evaluation of seedling and adult disease responses revealed that K16-730-3 is resistant to stripe rust and powdery mildew. In addition, no obvious difference in grain yield was observed between K16-730-3 and its wheat parents. Genotyping-by-sequencing analyses indicated that 74 PCR-based markers can accurately trace chromosome 4E, which were linked to the disease resistance genes in the wheat background. Further marker validation analyses revealed that 13 specific markers can distinguish between the E-genome chromosomes of Th. scirpeum and the chromosomes of other wheat-related species. The new substitution line K16-730-3 carrying the stripe rust and powdery mildew resistance genes will be useful as novel germplasm in breeding for disease resistance. The markers developed in this study can be used in marker-assisted selection for increasing disease resistance in wheat. SN - 0191-2917 UR - https://www.unboundmedicine.com/medline/citation/34698515/Development_and_Identification_of_a_Novel_Wheat_Thinopyrum_scirpeum_4E__4D__Chromosomal_Substitution_Line_with_Stripe_Rust_and_Powdery_Mildew_Resistance_ DB - PRIME DP - Unbound Medicine ER -
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