Tags

Type your tag names separated by a space and hit enter

Purification of xanthine dehydrogenase from rat liver: a rapid procedure with high enzyme yields.
Arch Biochem Biophys. 1987 Oct; 258(1):219-25.AB

Abstract

Xanthine dehydrogenase (EC 1.2.1.37) was purified approximately 1000-fold from liver homogenates of adult male Sprague-Dawley rats. Enzyme recovery was good (greater than 20% of the starting activity was obtained), and the homogeneously pure enzyme had a molecular mass of approximately 300,000 Da. The purified protein exhibited a specific activity of 2470 units/mg protein and spectral properties identical to those of the best preparations of this enzyme reported by other investigators. Routine preparations of this enzyme also possess higher dehydrogenase:oxidase ratios (typically between 5 and 6) than do other xanthine dehydrogenase preparations so far reported in the literature. Maximum dehydrogenase:oxidase ratios, greater than 10, could be obtained from this procedure if only peak dehydrogenase fractions from the chromatography columns were saved. The present small-scale purification method, which can be completed in 48-60 h, utilizes ammonium sulfate fractionation, Sephadex G-200 column chromatography, Blue Dextran-Sepharose column chromatography, and preparative gel electrophoresis.

Authors+Show Affiliations

Division of Environmental Health, School of Public Health, University of Minnesota, Minneapolis 55455.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3478001

Citation

Suleiman, S A., and J B. Stevens. "Purification of Xanthine Dehydrogenase From Rat Liver: a Rapid Procedure With High Enzyme Yields." Archives of Biochemistry and Biophysics, vol. 258, no. 1, 1987, pp. 219-25.
Suleiman SA, Stevens JB. Purification of xanthine dehydrogenase from rat liver: a rapid procedure with high enzyme yields. Arch Biochem Biophys. 1987;258(1):219-25.
Suleiman, S. A., & Stevens, J. B. (1987). Purification of xanthine dehydrogenase from rat liver: a rapid procedure with high enzyme yields. Archives of Biochemistry and Biophysics, 258(1), 219-25.
Suleiman SA, Stevens JB. Purification of Xanthine Dehydrogenase From Rat Liver: a Rapid Procedure With High Enzyme Yields. Arch Biochem Biophys. 1987;258(1):219-25. PubMed PMID: 3478001.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification of xanthine dehydrogenase from rat liver: a rapid procedure with high enzyme yields. AU - Suleiman,S A, AU - Stevens,J B, PY - 1987/10/1/pubmed PY - 1987/10/1/medline PY - 1987/10/1/entrez SP - 219 EP - 25 JF - Archives of biochemistry and biophysics JO - Arch Biochem Biophys VL - 258 IS - 1 N2 - Xanthine dehydrogenase (EC 1.2.1.37) was purified approximately 1000-fold from liver homogenates of adult male Sprague-Dawley rats. Enzyme recovery was good (greater than 20% of the starting activity was obtained), and the homogeneously pure enzyme had a molecular mass of approximately 300,000 Da. The purified protein exhibited a specific activity of 2470 units/mg protein and spectral properties identical to those of the best preparations of this enzyme reported by other investigators. Routine preparations of this enzyme also possess higher dehydrogenase:oxidase ratios (typically between 5 and 6) than do other xanthine dehydrogenase preparations so far reported in the literature. Maximum dehydrogenase:oxidase ratios, greater than 10, could be obtained from this procedure if only peak dehydrogenase fractions from the chromatography columns were saved. The present small-scale purification method, which can be completed in 48-60 h, utilizes ammonium sulfate fractionation, Sephadex G-200 column chromatography, Blue Dextran-Sepharose column chromatography, and preparative gel electrophoresis. SN - 0003-9861 UR - https://www.unboundmedicine.com/medline/citation/3478001/Purification_of_xanthine_dehydrogenase_from_rat_liver:_a_rapid_procedure_with_high_enzyme_yields_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0003-9861(87)90338-9 DB - PRIME DP - Unbound Medicine ER -