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Age and intraocular pressure in murine experimental glaucoma.
Prog Retin Eye Res. 2021 Nov 18 [Online ahead of print]PR

Abstract

Age and intraocular pressure (IOP) are the two most important risk factors for the development and progression of open-angle glaucoma. While IOP is commonly considered in models of experimental glaucoma (EG), most studies use juvenile or adult animals and seldom older animals which are representative of the human disease. This paper provides a concise review of how retinal ganglion cell (RGC) loss, the hallmark of glaucoma, can be evaluated in EG with a special emphasis on serial in vivo imaging, a parallel approach used in clinical practice. It appraises the suitability of EG models for the purpose of in vivo imaging and argues for the use of models that provide a sustained elevation of IOP, without compromise of the ocular media. In a study with parallel cohorts of adult (3-month-old, equivalent to 20 human years) and old (2-year-old, equivalent to 70 human years) mice, we compare the effects of elevated IOP on serial ganglion cell complex thickness and individual RGC dendritic morphology changes obtained in vivo. We also evaluate how age modulates the impact of elevated IOP on RGC somal and axonal density in histological analysis as well the density of melanopsin RGCs. We discuss the challenges of using old animals and emphasize the potential of single RGC imaging for understanding the pathobiology of RGC loss and evaluating new therapeutic avenues.

Authors+Show Affiliations

Retina and Optic Nerve Research Laboratory, Dalhousie University, Halifax, Nova Scotia, Canada.Retina and Optic Nerve Research Laboratory, Dalhousie University, Halifax, Nova Scotia, Canada; Department of Medical Neuroscience, Dalhousie University, Halifax, Nova Scotia, Canada.Department of Ophthalmology and Visual Sciences, Dalhousie University, Halifax, Nova Scotia, Canada.Retina and Optic Nerve Research Laboratory, Dalhousie University, Halifax, Nova Scotia, Canada.Retina and Optic Nerve Research Laboratory, Dalhousie University, Halifax, Nova Scotia, Canada.Retina and Optic Nerve Research Laboratory, Dalhousie University, Halifax, Nova Scotia, Canada; Department of Ophthalmology and Visual Sciences, Dalhousie University, Halifax, Nova Scotia, Canada.Retina and Optic Nerve Research Laboratory, Dalhousie University, Halifax, Nova Scotia, Canada; Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia, Canada.Retina and Optic Nerve Research Laboratory, Dalhousie University, Halifax, Nova Scotia, Canada; Department of Medical Neuroscience, Dalhousie University, Halifax, Nova Scotia, Canada; Department of Ophthalmology and Visual Sciences, Dalhousie University, Halifax, Nova Scotia, Canada; Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia, Canada. Electronic address: bal@dal.ca.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

34801667

Citation

Di Pierdomenico, Johnny, et al. "Age and Intraocular Pressure in Murine Experimental Glaucoma." Progress in Retinal and Eye Research, 2021, p. 101021.
Di Pierdomenico J, Henderson DCM, Giammaria S, et al. Age and intraocular pressure in murine experimental glaucoma. Prog Retin Eye Res. 2021.
Di Pierdomenico, J., Henderson, D. C. M., Giammaria, S., Smith, V. L., Jamet, A. J., Smith, C. A., Hooper, M. L., & Chauhan, B. C. (2021). Age and intraocular pressure in murine experimental glaucoma. Progress in Retinal and Eye Research, 101021. https://doi.org/10.1016/j.preteyeres.2021.101021
Di Pierdomenico J, et al. Age and Intraocular Pressure in Murine Experimental Glaucoma. Prog Retin Eye Res. 2021 Nov 18;101021. PubMed PMID: 34801667.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Age and intraocular pressure in murine experimental glaucoma. AU - Di Pierdomenico,Johnny, AU - Henderson,Delaney C M, AU - Giammaria,Sara, AU - Smith,Victoria L, AU - Jamet,Aliénor J, AU - Smith,Corey A, AU - Hooper,Michele L, AU - Chauhan,Balwantray C, Y1 - 2021/11/18/ PY - 2021/08/13/received PY - 2021/10/25/revised PY - 2021/11/08/accepted PY - 2021/11/22/pubmed PY - 2021/11/22/medline PY - 2021/11/21/entrez KW - Age KW - Experimental glaucoma KW - In vivo imaging KW - Intraocular pressure KW - Mouse KW - Retinal ganglion cell SP - 101021 EP - 101021 JF - Progress in retinal and eye research JO - Prog Retin Eye Res N2 - Age and intraocular pressure (IOP) are the two most important risk factors for the development and progression of open-angle glaucoma. While IOP is commonly considered in models of experimental glaucoma (EG), most studies use juvenile or adult animals and seldom older animals which are representative of the human disease. This paper provides a concise review of how retinal ganglion cell (RGC) loss, the hallmark of glaucoma, can be evaluated in EG with a special emphasis on serial in vivo imaging, a parallel approach used in clinical practice. It appraises the suitability of EG models for the purpose of in vivo imaging and argues for the use of models that provide a sustained elevation of IOP, without compromise of the ocular media. In a study with parallel cohorts of adult (3-month-old, equivalent to 20 human years) and old (2-year-old, equivalent to 70 human years) mice, we compare the effects of elevated IOP on serial ganglion cell complex thickness and individual RGC dendritic morphology changes obtained in vivo. We also evaluate how age modulates the impact of elevated IOP on RGC somal and axonal density in histological analysis as well the density of melanopsin RGCs. We discuss the challenges of using old animals and emphasize the potential of single RGC imaging for understanding the pathobiology of RGC loss and evaluating new therapeutic avenues. SN - 1873-1635 UR - https://www.unboundmedicine.com/medline/citation/34801667/Age_and_intraocular_pressure_in_murine_experimental_glaucoma. L2 - https://linkinghub.elsevier.com/retrieve/pii/S1350-9462(21)00082-3 DB - PRIME DP - Unbound Medicine ER -
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