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TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line.
Front Plant Sci. 2021; 12:762265.FP

Abstract

Triticum boeoticum Boiss (AbAb, 2n = 2x = 14) is one of the sources of the blue grain trait controlled by blue aleurone layer 2 (Ba2). However, the underlying genes have not been cloned. In this study, a transcriptomic comparison between a blue-grained wheat-T. boeoticum substitution line and its wheat parent identified 41 unigenes related to anthocyanin biosynthesis and 29 unigenes related to transport. The bHLH transcription factor gene TbMYC4A showed a higher expression level in the blue-grained substitution line. TbMYC4A contained the three characteristic bHLH transcription factor domains (bHLH-MYC_N, HLH and ACT-like) and clustered with genes identified from other wheat lines with the blue grain trait derived from other Triticeae species. TbMYC4A overexpression confirmed that it was a functional bHLH transcription factor. The analysis of a TbMYC4A-specific marker showed that the gene was also present in T. boeoticum and T. monococcum with blue aleurone but absent in other Triticeae materials with white aleurone. These results indicate that TbMYC4A is a candidate gene of Ba2 controlling the blue aleurone trait. The isolation of TbMYC4A is helpful for further clarifying the genetic mechanism of the blue aleurone trait and is of great significance for breeding blue-grained wheat varieties.

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Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.

Qinghai Province Key Laboratory of Crop Molecular Breeding, Xining, China. Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34804098

Citation

Liu, Xin, et al. "TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum Boeoticum Substitution Line." Frontiers in Plant Science, vol. 12, 2021, p. 762265.

Liu X, Zhang M, Jiang X, et al. TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line. Front Plant Sci. 2021;12:762265.

Liu, X., Zhang, M., Jiang, X., Li, H., Jia, Z., Hao, M., Jiang, B., Huang, L., Ning, S., Yuan, Z., Chen, X., Chen, X., Liu, D., Liu, B., & Zhang, L. (2021). TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line. Frontiers in Plant Science, 12, 762265. https://doi.org/10.3389/fpls.2021.762265

Liu X, et al. TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum Boeoticum Substitution Line. Front Plant Sci. 2021;12:762265. PubMed PMID: 34804098.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line. AU - Liu,Xin, AU - Zhang,Minghu, AU - Jiang,Xiaomei, AU - Li,Hui, AU - Jia,Zhenjiao, AU - Hao,Ming, AU - Jiang,Bo, AU - Huang,Lin, AU - Ning,Shunzong, AU - Yuan,Zhongwei, AU - Chen,Xuejiao, AU - Chen,Xue, AU - Liu,Dengcai, AU - Liu,Baolong, AU - Zhang,Lianquan, Y1 - 2021/11/05/ PY - 2021/08/21/received PY - 2021/10/12/accepted PY - 2021/11/22/entrez PY - 2021/11/23/pubmed PY - 2021/11/23/medline KW - TbMYC4A KW - Triticum boeoticum KW - anthocyanin biosynthesis KW - blue aleurone KW - transcriptome analysis SP - 762265 EP - 762265 JF - Frontiers in plant science JO - Front Plant Sci VL - 12 N2 - Triticum boeoticum Boiss (AbAb, 2n = 2x = 14) is one of the sources of the blue grain trait controlled by blue aleurone layer 2 (Ba2). However, the underlying genes have not been cloned. In this study, a transcriptomic comparison between a blue-grained wheat-T. boeoticum substitution line and its wheat parent identified 41 unigenes related to anthocyanin biosynthesis and 29 unigenes related to transport. The bHLH transcription factor gene TbMYC4A showed a higher expression level in the blue-grained substitution line. TbMYC4A contained the three characteristic bHLH transcription factor domains (bHLH-MYC_N, HLH and ACT-like) and clustered with genes identified from other wheat lines with the blue grain trait derived from other Triticeae species. TbMYC4A overexpression confirmed that it was a functional bHLH transcription factor. The analysis of a TbMYC4A-specific marker showed that the gene was also present in T. boeoticum and T. monococcum with blue aleurone but absent in other Triticeae materials with white aleurone. These results indicate that TbMYC4A is a candidate gene of Ba2 controlling the blue aleurone trait. The isolation of TbMYC4A is helpful for further clarifying the genetic mechanism of the blue aleurone trait and is of great significance for breeding blue-grained wheat varieties. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/34804098/TbMYC4A_Is_a_Candidate_Gene_Controlling_the_Blue_Aleurone_Trait_in_a_Wheat_Triticum_boeoticum_Substitution_Line_ DB - PRIME DP - Unbound Medicine ER -