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Evaluation of a quality control material containing hemoglobin for blood gas and pH measurement.
Scand J Clin Lab Invest Suppl. 1987; 188:101-12.SJ

Abstract

A procedure for the preparation of a Stroma-Free Hemoglobin Solution (SFHS) is given. The stability of this SFHS containing Methemoglobin Reductase, can be improved by addition of NADH. The characteristics of the stable SFHS can be manipulated by varying independently the concentrations of bicarbonate and Inositol-Hexa-Phosphate. This way the desired acid-base behaviour and position of the Oxygen Hemoglobin Equilibrium Curve (OHEC) can be obtained. Three SFHS were prepared with acidotic, alkalotic or normal acid-base characteristics and all SFHS had an OHEC in the normal position (actual p50 3.33-3.87 kPa). Results show that: stability of SFHS is 1 year, xHi less than 2.5% after 1 year; p50 decreases about 15% per year; Hill's coefficient, nHill, is constant, but differed between levels; the mean values for nHill are 2.0 for the acidotic level, 2.2 for the normal and 2.4 for the alkalotic level; temperature coefficients for SFHS are: d(pH)/dt = -0.016 pH/degrees C, d(pCO2)/dt = 6.2%/degrees C and d(pO2)/dt = 7.2%/degrees C. Oxygen and carbon dioxide tonometered SFHS, of which the pH was measured with the reference method for pH measurement in blood was used on several blood gas analyzers to demonstrate the suitability for pH, pCO2 and pO2 measurement. The SFHS, which contained oxyhemoglobin, carboxyhemoglobin and methemoglobin, was also used as a control material for hemoglobin meters and CO-Oximeters. It is concluded that SFHS behaves blood-like with respect to pH, pCO2 and pO2 as well as total hemoglobin, oxygen saturation, carboxyhemoglobin and methemoglobin measurements. In contrast to hemoglobin-free aqueous control material, it buffers oxygen in a blood-like manner. Its shelflife is limited compared to the generally used aqueous control materials, but it is sufficient for repetitive use in clinical laboratories.

Authors+Show Affiliations

Sprokholt R
Heart-Lung Institute, Univ. Hospital Utrecht, The Netherlands.
van Ooik S
No affiliation info available
van den Camp RA
No affiliation info available
Bouma BN
No affiliation info available
Zijlstra WG
No affiliation info available
Maas AH
No affiliation info available

MeSH

Blood Gas AnalysisCarboxyhemoglobinEvaluation Studies as TopicHemoglobinsHydrogen-Ion ConcentrationMethemoglobinOxyhemoglobinsQuality ControlReference StandardsSpectrophotometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3482476

Citation

Sprokholt, R, et al. "Evaluation of a Quality Control Material Containing Hemoglobin for Blood Gas and pH Measurement." Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum, vol. 188, 1987, pp. 101-12.
Sprokholt R, van Ooik S, van den Camp RA, et al. Evaluation of a quality control material containing hemoglobin for blood gas and pH measurement. Scand J Clin Lab Invest Suppl. 1987;188:101-12.
Sprokholt, R., van Ooik, S., van den Camp, R. A., Bouma, B. N., Zijlstra, W. G., & Maas, A. H. (1987). Evaluation of a quality control material containing hemoglobin for blood gas and pH measurement. Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum, 188, 101-12.
Sprokholt R, et al. Evaluation of a Quality Control Material Containing Hemoglobin for Blood Gas and pH Measurement. Scand J Clin Lab Invest Suppl. 1987;188:101-12. PubMed PMID: 3482476.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of a quality control material containing hemoglobin for blood gas and pH measurement. AU - Sprokholt,R, AU - van Ooik,S, AU - van den Camp,R A, AU - Bouma,B N, AU - Zijlstra,W G, AU - Maas,A H, PY - 1987/1/1/pubmed PY - 1987/1/1/medline PY - 1987/1/1/entrez SP - 101 EP - 12 JF - Scandinavian journal of clinical and laboratory investigation. Supplementum JO - Scand J Clin Lab Invest Suppl VL - 188 N2 - A procedure for the preparation of a Stroma-Free Hemoglobin Solution (SFHS) is given. The stability of this SFHS containing Methemoglobin Reductase, can be improved by addition of NADH. The characteristics of the stable SFHS can be manipulated by varying independently the concentrations of bicarbonate and Inositol-Hexa-Phosphate. This way the desired acid-base behaviour and position of the Oxygen Hemoglobin Equilibrium Curve (OHEC) can be obtained. Three SFHS were prepared with acidotic, alkalotic or normal acid-base characteristics and all SFHS had an OHEC in the normal position (actual p50 3.33-3.87 kPa). Results show that: stability of SFHS is 1 year, xHi less than 2.5% after 1 year; p50 decreases about 15% per year; Hill's coefficient, nHill, is constant, but differed between levels; the mean values for nHill are 2.0 for the acidotic level, 2.2 for the normal and 2.4 for the alkalotic level; temperature coefficients for SFHS are: d(pH)/dt = -0.016 pH/degrees C, d(pCO2)/dt = 6.2%/degrees C and d(pO2)/dt = 7.2%/degrees C. Oxygen and carbon dioxide tonometered SFHS, of which the pH was measured with the reference method for pH measurement in blood was used on several blood gas analyzers to demonstrate the suitability for pH, pCO2 and pO2 measurement. The SFHS, which contained oxyhemoglobin, carboxyhemoglobin and methemoglobin, was also used as a control material for hemoglobin meters and CO-Oximeters. It is concluded that SFHS behaves blood-like with respect to pH, pCO2 and pO2 as well as total hemoglobin, oxygen saturation, carboxyhemoglobin and methemoglobin measurements. In contrast to hemoglobin-free aqueous control material, it buffers oxygen in a blood-like manner. Its shelflife is limited compared to the generally used aqueous control materials, but it is sufficient for repetitive use in clinical laboratories. SN - 0085-591X UR - https://www.unboundmedicine.com/medline/citation/3482476/Evaluation_of_a_quality_control_material_containing_hemoglobin_for_blood_gas_and_pH_measurement_ DB - PRIME DP - Unbound Medicine ER -