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Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract.
Viruses. 2021 11 02; 13(11)V

Abstract

The COVID-19 pandemic has currently created an unprecedented threat to human society and global health. A rapid mass vaccination to create herd immunity against SARS-CoV-2 is a crucial measure to ease the spread of this disease. Here, we investigated the immunogenicity of a SARS-CoV-2 subunit vaccine candidate, a SARS-CoV-2 spike glycoprotein encapsulated in N,N,N-trimethyl chitosan particles or S-TMC NPs. Upon intraperitoneal immunization, S-TMC NP-immunized mice elicited a stronger systemic antibody response, with neutralizing capacity against SARS-CoV-2, than mice receiving the soluble form of S-glycoprotein. S-TMC NPs were able to stimulate the circulating IgG and IgA as found in SARS-CoV-2-infected patients. In addition, spike-specific T cell responses were drastically activated in S-TMC NP-immunized mice. Surprisingly, administration of S-TMC NPs via the intraperitoneal route also stimulated SARS-CoV-2-specific immune responses in the respiratory tract, which were demonstrated by the presence of high levels of SARS-CoV-2-specific IgG and IgA in the lung homogenates and bronchoalveolar lavages of the immunized mice. We found that peritoneal immunization with spike nanospheres stimulates both systemic and respiratory mucosal immunity.

Authors+Show Affiliations

Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.Institute of Biological Products, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand.Institute of Biological Products, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand.Department of Chemistry, Faculty of Science, Mahidol University, Salaya, Nakornpatom 73170, Thailand.Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

34835008

Citation

Jearanaiwitayakul, Tuksin, et al. "Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses Against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract." Viruses, vol. 13, no. 11, 2021.
Jearanaiwitayakul T, Apichirapokey S, Chawengkirttikul R, et al. Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract. Viruses. 2021;13(11).
Jearanaiwitayakul, T., Apichirapokey, S., Chawengkirttikul, R., Limthongkul, J., Seesen, M., Jakaew, P., Trisiriwanich, S., Sapsutthipas, S., Sunintaboon, P., & Ubol, S. (2021). Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract. Viruses, 13(11). https://doi.org/10.3390/v13112202
Jearanaiwitayakul T, et al. Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses Against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract. Viruses. 2021 11 2;13(11) PubMed PMID: 34835008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract. AU - Jearanaiwitayakul,Tuksin, AU - Apichirapokey,Suttikarn, AU - Chawengkirttikul,Runglawan, AU - Limthongkul,Jitra, AU - Seesen,Mathurin, AU - Jakaew,Phissinee, AU - Trisiriwanich,Sakalin, AU - Sapsutthipas,Sompong, AU - Sunintaboon,Panya, AU - Ubol,Sukathida, Y1 - 2021/11/02/ PY - 2021/09/07/received PY - 2021/10/26/revised PY - 2021/10/29/accepted PY - 2021/11/27/entrez PY - 2021/11/28/pubmed PY - 2021/12/15/medline KW - COVID-19 vaccine KW - SARS-CoV-2 spike glycoprotein KW - adjuvant delivery particles KW - mucosal immunity KW - peritoneal immunization JF - Viruses JO - Viruses VL - 13 IS - 11 N2 - The COVID-19 pandemic has currently created an unprecedented threat to human society and global health. A rapid mass vaccination to create herd immunity against SARS-CoV-2 is a crucial measure to ease the spread of this disease. Here, we investigated the immunogenicity of a SARS-CoV-2 subunit vaccine candidate, a SARS-CoV-2 spike glycoprotein encapsulated in N,N,N-trimethyl chitosan particles or S-TMC NPs. Upon intraperitoneal immunization, S-TMC NP-immunized mice elicited a stronger systemic antibody response, with neutralizing capacity against SARS-CoV-2, than mice receiving the soluble form of S-glycoprotein. S-TMC NPs were able to stimulate the circulating IgG and IgA as found in SARS-CoV-2-infected patients. In addition, spike-specific T cell responses were drastically activated in S-TMC NP-immunized mice. Surprisingly, administration of S-TMC NPs via the intraperitoneal route also stimulated SARS-CoV-2-specific immune responses in the respiratory tract, which were demonstrated by the presence of high levels of SARS-CoV-2-specific IgG and IgA in the lung homogenates and bronchoalveolar lavages of the immunized mice. We found that peritoneal immunization with spike nanospheres stimulates both systemic and respiratory mucosal immunity. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/34835008/Peritoneal_Administration_of_a_Subunit_Vaccine_Encapsulated_in_a_Nanodelivery_System_Not_Only_Augments_Systemic_Responses_against_SARS_CoV_2_but_Also_Stimulates_Responses_in_the_Respiratory_Tract_ DB - PRIME DP - Unbound Medicine ER -