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Wheat genetic loci conferring resistance to stripe rust in the face of genetically diverse races of the fungus Puccinia striiformis f. sp. tritici.
Theor Appl Genet. 2022 Jan; 135(1):301-319.TA

Abstract

KEY MESSAGE

Analysis of a wheat multi-founder population identified 14 yellow rust resistance QTL. For three of the four most significant QTL, haplotype analysis indicated resistance alleles were rare in European wheat. Stripe rust, or yellow rust (YR), is a major fungal disease of wheat (Triticum aestivum) caused by Puccinia striiformis Westend f. sp. tritici (Pst). Since 2011, the historically clonal European Pst races have been superseded by the rapid incursion of genetically diverse lineages, reducing the resistance of varieties previously showing durable resistance. Identification of sources of genetic resistance to such races is a high priority for wheat breeding. Here we use a wheat eight-founder multi-parent population genotyped with a 90,000 feature single nucleotide polymorphism array to genetically map YR resistance to such new Pst races. Genetic analysis of five field trials at three UK sites identified 14 quantitative trait loci (QTL) conferring resistance. Of these, four highly significant loci were consistently identified across all test environments, located on chromosomes 1A (QYr.niab-1A.1), 2A (QYr.niab-2A.1), 2B (QYr.niab-2B.1) and 2D (QYr.niab-2D.1), together explaining ~ 50% of the phenotypic variation. Analysis of these four QTL in two-way and three-way combinations showed combinations conferred greater resistance than single QTL, and genetic markers were developed that distinguished resistant and susceptible alleles. Haplotype analysis in a collection of wheat varieties found that the haplotypes associated with YR resistance at three of these four major loci were rare (≤ 7%) in European wheat, highlighting their potential utility for future targeted improvement of disease resistance. Notably, the physical interval for QTL QYr.niab-2B.1 contained five nucleotide-binding leucine-rich repeat candidate genes with integrated BED domains, of which two corresponded to the cloned resistance genes Yr7 and Yr5/YrSp.

Authors+Show Affiliations

NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK. Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, CB2 3EA, UK.NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK.Limagrain UK Ltd, Market Rasen, LN7 6DT, UK.Limagrain UK Ltd, Market Rasen, LN7 6DT, UK.NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK.Scotland's Rural College (SRUC), Kings Buildings, West Mains Road, Edinburgh, EH9 3JG, UK.NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK.NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK. Scotland's Rural College (SRUC), The King's Buildings, West Mains Road, Edinburgh, EH9 3JG, UK.NIAB, 93 Lawrence Weaver Road, Cambridge, CB3 0LE, UK. james.cockram@niab.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34837509

Citation

Bouvet, Laura, et al. "Wheat Genetic Loci Conferring Resistance to Stripe Rust in the Face of Genetically Diverse Races of the Fungus Puccinia Striiformis F. Sp. Tritici." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 135, no. 1, 2022, pp. 301-319.
Bouvet L, Percival-Alwyn L, Berry S, et al. Wheat genetic loci conferring resistance to stripe rust in the face of genetically diverse races of the fungus Puccinia striiformis f. sp. tritici. Theor Appl Genet. 2022;135(1):301-319.
Bouvet, L., Percival-Alwyn, L., Berry, S., Fenwick, P., Mantello, C. C., Sharma, R., Holdgate, S., Mackay, I. J., & Cockram, J. (2022). Wheat genetic loci conferring resistance to stripe rust in the face of genetically diverse races of the fungus Puccinia striiformis f. sp. tritici. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 135(1), 301-319. https://doi.org/10.1007/s00122-021-03967-z
Bouvet L, et al. Wheat Genetic Loci Conferring Resistance to Stripe Rust in the Face of Genetically Diverse Races of the Fungus Puccinia Striiformis F. Sp. Tritici. Theor Appl Genet. 2022;135(1):301-319. PubMed PMID: 34837509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Wheat genetic loci conferring resistance to stripe rust in the face of genetically diverse races of the fungus Puccinia striiformis f. sp. tritici. AU - Bouvet,Laura, AU - Percival-Alwyn,Lawrence, AU - Berry,Simon, AU - Fenwick,Paul, AU - Mantello,Camila Campos, AU - Sharma,Rajiv, AU - Holdgate,Sarah, AU - Mackay,Ian J, AU - Cockram,James, Y1 - 2021/11/27/ PY - 2021/04/23/received PY - 2021/10/05/accepted PY - 2021/11/28/pubmed PY - 2022/3/18/medline PY - 2021/11/27/entrez SP - 301 EP - 319 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 135 IS - 1 N2 - KEY MESSAGE: Analysis of a wheat multi-founder population identified 14 yellow rust resistance QTL. For three of the four most significant QTL, haplotype analysis indicated resistance alleles were rare in European wheat. Stripe rust, or yellow rust (YR), is a major fungal disease of wheat (Triticum aestivum) caused by Puccinia striiformis Westend f. sp. tritici (Pst). Since 2011, the historically clonal European Pst races have been superseded by the rapid incursion of genetically diverse lineages, reducing the resistance of varieties previously showing durable resistance. Identification of sources of genetic resistance to such races is a high priority for wheat breeding. Here we use a wheat eight-founder multi-parent population genotyped with a 90,000 feature single nucleotide polymorphism array to genetically map YR resistance to such new Pst races. Genetic analysis of five field trials at three UK sites identified 14 quantitative trait loci (QTL) conferring resistance. Of these, four highly significant loci were consistently identified across all test environments, located on chromosomes 1A (QYr.niab-1A.1), 2A (QYr.niab-2A.1), 2B (QYr.niab-2B.1) and 2D (QYr.niab-2D.1), together explaining ~ 50% of the phenotypic variation. Analysis of these four QTL in two-way and three-way combinations showed combinations conferred greater resistance than single QTL, and genetic markers were developed that distinguished resistant and susceptible alleles. Haplotype analysis in a collection of wheat varieties found that the haplotypes associated with YR resistance at three of these four major loci were rare (≤ 7%) in European wheat, highlighting their potential utility for future targeted improvement of disease resistance. Notably, the physical interval for QTL QYr.niab-2B.1 contained five nucleotide-binding leucine-rich repeat candidate genes with integrated BED domains, of which two corresponded to the cloned resistance genes Yr7 and Yr5/YrSp. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/34837509/Wheat_genetic_loci_conferring_resistance_to_stripe_rust_in_the_face_of_genetically_diverse_races_of_the_fungus_Puccinia_striiformis_f__sp__tritici_ L2 - https://dx.doi.org/10.1007/s00122-021-03967-z DB - PRIME DP - Unbound Medicine ER -