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Detection of Orientia tsutsugamushi in Novel Trombiculid Mite Species in Northern Tamil Nadu, India: Use of Targeting the Multicopy traD Gene.
J Med Entomol. 2022 03 16; 59(2):693-699.JM

Abstract

Detection of Orientia tsutsugamushi DNA in a trombiculid mite chigger species suggests that it might be a potential vector of scrub typhus in an endemic area. Over a period of 20 mo, 85 rats were trapped, 57 had chiggers that were identified by standard morphometric techniques. The chigger pools were assessed by performing PCR assays targeting fragments of the single-copy genes 56 kDa type-specific antigen gene (TSA56) by nested PCR and the 47 kDa (htrA) quantitative real-time PCR (qPCR). The novel traD SYBR green assay that detects a multicopy gene was also performed. In total, 27 chigger pools were positive by traD qPCR, of which only 7 were positive by 47 kDa qPCR and in 3 of these, 56 kDa gene was amplified by nested PCR. Orientia tsutsugamushi-specific DNA was detected in Ascoschoengastia spp., Schoengastiella ligula, Leptotrombidium rajasthanense, Leptotrombidium deliense, and Leptotrombidium jayawickremei chigger pools. Therefore, they could be potential vectors of scrub typhus in Southern India. The three 56 kDa sequences belonged to TA716 genotype and Kato genogroup. Further studies are needed to confirm these chigger species as scrub typhus vectors in Northern Tamil Nadu.

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Authors+Show Affiliations

Prakash JAJ
Department of Clinical Microbiology, Christian Medical College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India.
Kamarasu K
Institute of Vector Control and Zoonoses, 150 Titan Jewellery Road, SIPCOT Phase I, Hosur Tamil Nadu 635126, India.
Samuel PP
ICMR-Vector Control Research Centre Field Station, 4, Sarojini St, Chinna Chokikulam, Madurai Tamil Nadu 625002, India.
Govindarajan R
ICMR-Vector Control Research Centre Field Station, 4, Sarojini St, Chinna Chokikulam, Madurai Tamil Nadu 625002, India.
Govindasamy P
Department of Clinical Microbiology, Christian Medical College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India.
Johnson LA
Department of Clinical Microbiology, Christian Medical College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India.
Ramalingam P
Institute of Vector Control and Zoonoses, 150 Titan Jewellery Road, SIPCOT Phase I, Hosur Tamil Nadu 635126, India.
Nirmalson J
Institute of Vector Control and Zoonoses, 150 Titan Jewellery Road, SIPCOT Phase I, Hosur Tamil Nadu 635126, India.
Seran KC
Department of Community Medicine, Madras Medical College, Near Park Town Station, Chennai, Tamil Nadu 600003, India.

MeSH

AnimalsIndiaOrientia tsutsugamushiRatsReal-Time Polymerase Chain ReactionRodent DiseasesRodentiaScrub TyphusTrombiculidae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

34850037

Citation

Prakash, John Antony Jude, et al. "Detection of Orientia Tsutsugamushi in Novel Trombiculid Mite Species in Northern Tamil Nadu, India: Use of Targeting the Multicopy traD Gene." Journal of Medical Entomology, vol. 59, no. 2, 2022, pp. 693-699.
Prakash JAJ, Kamarasu K, Samuel PP, et al. Detection of Orientia tsutsugamushi in Novel Trombiculid Mite Species in Northern Tamil Nadu, India: Use of Targeting the Multicopy traD Gene. J Med Entomol. 2022;59(2):693-699.
Prakash, J. A. J., Kamarasu, K., Samuel, P. P., Govindarajan, R., Govindasamy, P., Johnson, L. A., Ramalingam, P., Nirmalson, J., & Seran, K. C. (2022). Detection of Orientia tsutsugamushi in Novel Trombiculid Mite Species in Northern Tamil Nadu, India: Use of Targeting the Multicopy traD Gene. Journal of Medical Entomology, 59(2), 693-699. https://doi.org/10.1093/jme/tjab180
Prakash JAJ, et al. Detection of Orientia Tsutsugamushi in Novel Trombiculid Mite Species in Northern Tamil Nadu, India: Use of Targeting the Multicopy traD Gene. J Med Entomol. 2022 03 16;59(2):693-699. PubMed PMID: 34850037.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Orientia tsutsugamushi in Novel Trombiculid Mite Species in Northern Tamil Nadu, India: Use of Targeting the Multicopy traD Gene. AU - Prakash,John Antony Jude, AU - Kamarasu,Kannan, AU - Samuel,P Philip, AU - Govindarajan,Renu, AU - Govindasamy,Punitha, AU - Johnson,Liji Anna, AU - Ramalingam,P, AU - Nirmalson,J, AU - Seran,K C, PY - 2021/07/02/received PY - 2021/12/2/pubmed PY - 2022/5/10/medline PY - 2021/12/1/entrez KW - Ascoschoengastia KW - Leptotrombidium KW - Schoengastiella KW - traD KW - scrub typhus SP - 693 EP - 699 JF - Journal of medical entomology JO - J Med Entomol VL - 59 IS - 2 N2 - Detection of Orientia tsutsugamushi DNA in a trombiculid mite chigger species suggests that it might be a potential vector of scrub typhus in an endemic area. Over a period of 20 mo, 85 rats were trapped, 57 had chiggers that were identified by standard morphometric techniques. The chigger pools were assessed by performing PCR assays targeting fragments of the single-copy genes 56 kDa type-specific antigen gene (TSA56) by nested PCR and the 47 kDa (htrA) quantitative real-time PCR (qPCR). The novel traD SYBR green assay that detects a multicopy gene was also performed. In total, 27 chigger pools were positive by traD qPCR, of which only 7 were positive by 47 kDa qPCR and in 3 of these, 56 kDa gene was amplified by nested PCR. Orientia tsutsugamushi-specific DNA was detected in Ascoschoengastia spp., Schoengastiella ligula, Leptotrombidium rajasthanense, Leptotrombidium deliense, and Leptotrombidium jayawickremei chigger pools. Therefore, they could be potential vectors of scrub typhus in Southern India. The three 56 kDa sequences belonged to TA716 genotype and Kato genogroup. Further studies are needed to confirm these chigger species as scrub typhus vectors in Northern Tamil Nadu. SN - 1938-2928 UR - https://www.unboundmedicine.com/medline/citation/34850037/Detection_of_Orientia_tsutsugamushi_in_Novel_Trombiculid_Mite_Species_in_Northern_Tamil_Nadu_India:_Use_of_Targeting_the_Multicopy_traD_Gene_ DB - PRIME DP - Unbound Medicine ER -