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[Electroacupuncture improves behavioral activities by suppressing neuroinflammation and TLR4/NF-κB signaling in substantia nigra of midbrain in Parkinson's disease rats].
Zhen Ci Yan Jiu. 2021 Nov 25; 46(11):929-34.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture(EA) at "Fengfu" (GV16) and "Taichong"(LR3) on the expression of tyrosine hydroxylase (TH), α-synuclein (α-syn) and microglial-related microglial (MG), Toll-like receptor 4(TLR4)/nuclear factor kappa B (NF-κB) signaling pathway in the substantia nigra (SN) of midbrain in Rotenone-induced Parkinson's disease (PD) rats, so as to explore its mechanisms underlying improvement of PD.

METHODS

SD male rats were randomly divided into normal control, PD model and EA groups (n=12 in each group). The PD model was established by subcutaneous injection of rotenone (1 mg/kg) at the back of neck. EA (2 Hz, 1 mA) was applied to bilateral GV16 and LR3, once daily for 2 weeks. The rats' behavior(hair color, reaction capacity, locomotion and gait state)scores (0-10 points) were given and the autonomic movement state (trajectory of autonomous motion, total distance, average speed and duration of motion in 8 min) was detected by using open field tests. The immunoactivity of TH and α-syn in the SN tissue were determined by using immunohistochemistry staining, and the number of Iba-1-labelled microglia (MG) was detected by using immunofluorescence staining. The expression levels of TLR4 and NF-κB P65 proteins in the SN were detected by Western blot, and the contents of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the SN were determined by using enzyme-linked immunosorbent assay.

RESULTS

In comparison with the normal group, the behavioral score, α-syn immunoactivity, number of Iba-1 labelled microglia, expression of TLR4 and NF-κB P65 proteins and the contents of TNF-α and IL-6 in the SN were significantly increased (P<0.01), whereas the total distance, average speed, duration of motion of the autonomic movement in 8 min, and the TH immunoactivity were remarkably decreased in the model group (P<0.01). After EA intervention, compared with the model group, the increase of the behavioral score, α-syn immunoactivity, number of Iba-1-labelled microglia, expression of TLR4 and NF-κB P65 proteins, the contents of TNF-α and IL-6 in the SN, and the decrease of the total distance, average speed, duration of motion of the autonomic movement in 8 min, and the TH immunoactivity were reversed (P<0.05, P<0.01).

CONCLUSION

EA can improve the behavioral manifestations of PD rats, which may be associated with its functions in down-regulating the abnormal accumulation of pathological α-syn, TLR4/NF-κB signaling, inhibiting activities of microglia and in up-regulating the expression of TH in the SN of midbrain.

Authors+Show Affiliations

College of Acupuncture-moxibustion and Orthopaedics, Hubei University of Chinese Medicine, Wuhan 430065, China;Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-Hmoxibustion, Wuhan 430065, China.College of Acupuncture-moxibustion and Orthopaedics, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-Hmoxibustion, Wuhan 430065, China.College of Acupuncture-moxibustion and Orthopaedics, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-Hmoxibustion, Wuhan 430065, China.College of Acupuncture-moxibustion and Orthopaedics, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-Hmoxibustion, Wuhan 430065, China.College of Acupuncture-moxibustion and Orthopaedics, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Provincial Collaborative Innovation Center of Preventive Treatment of Diseases by Acupuncture-Hmoxibustion, Wuhan 430065, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

34865329

Citation

Qi, Ling, et al. "[Electroacupuncture Improves Behavioral Activities By Suppressing Neuroinflammation and TLR4/NF-κB Signaling in Substantia Nigra of Midbrain in Parkinson's Disease Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 46, no. 11, 2021, pp. 929-34.
Qi L, Wang Y, Li YN, et al. [Electroacupuncture improves behavioral activities by suppressing neuroinflammation and TLR4/NF-κB signaling in substantia nigra of midbrain in Parkinson's disease rats]. Zhen Ci Yan Jiu. 2021;46(11):929-34.
Qi, L., Wang, Y., Li, Y. N., Guo, L., & Ma, J. (2021). [Electroacupuncture improves behavioral activities by suppressing neuroinflammation and TLR4/NF-κB signaling in substantia nigra of midbrain in Parkinson's disease rats]. Zhen Ci Yan Jiu = Acupuncture Research, 46(11), 929-34. https://doi.org/10.13702/j.1000-0607.200874
Qi L, et al. [Electroacupuncture Improves Behavioral Activities By Suppressing Neuroinflammation and TLR4/NF-κB Signaling in Substantia Nigra of Midbrain in Parkinson's Disease Rats]. Zhen Ci Yan Jiu. 2021 Nov 25;46(11):929-34. PubMed PMID: 34865329.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Electroacupuncture improves behavioral activities by suppressing neuroinflammation and TLR4/NF-κB signaling in substantia nigra of midbrain in Parkinson's disease rats]. AU - Qi,Ling, AU - Wang,Yao, AU - Li,Ya-Nan, AU - Guo,Lei, AU - Ma,Jun, PY - 2021/12/5/entrez PY - 2021/12/6/pubmed PY - 2021/12/6/medline KW - Electroacupuncture KW - Inflammatory factor KW - Microglia KW - Nuclear factor kappa B KW - Parkinson’s Disease KW - Toll-like receptor 4 SP - 929 EP - 34 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 46 IS - 11 N2 - OBJECTIVE: To observe the effect of electroacupuncture(EA) at "Fengfu" (GV16) and "Taichong"(LR3) on the expression of tyrosine hydroxylase (TH), α-synuclein (α-syn) and microglial-related microglial (MG), Toll-like receptor 4(TLR4)/nuclear factor kappa B (NF-κB) signaling pathway in the substantia nigra (SN) of midbrain in Rotenone-induced Parkinson's disease (PD) rats, so as to explore its mechanisms underlying improvement of PD. METHODS: SD male rats were randomly divided into normal control, PD model and EA groups (n=12 in each group). The PD model was established by subcutaneous injection of rotenone (1 mg/kg) at the back of neck. EA (2 Hz, 1 mA) was applied to bilateral GV16 and LR3, once daily for 2 weeks. The rats' behavior(hair color, reaction capacity, locomotion and gait state)scores (0-10 points) were given and the autonomic movement state (trajectory of autonomous motion, total distance, average speed and duration of motion in 8 min) was detected by using open field tests. The immunoactivity of TH and α-syn in the SN tissue were determined by using immunohistochemistry staining, and the number of Iba-1-labelled microglia (MG) was detected by using immunofluorescence staining. The expression levels of TLR4 and NF-κB P65 proteins in the SN were detected by Western blot, and the contents of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the SN were determined by using enzyme-linked immunosorbent assay. RESULTS: In comparison with the normal group, the behavioral score, α-syn immunoactivity, number of Iba-1 labelled microglia, expression of TLR4 and NF-κB P65 proteins and the contents of TNF-α and IL-6 in the SN were significantly increased (P<0.01), whereas the total distance, average speed, duration of motion of the autonomic movement in 8 min, and the TH immunoactivity were remarkably decreased in the model group (P<0.01). After EA intervention, compared with the model group, the increase of the behavioral score, α-syn immunoactivity, number of Iba-1-labelled microglia, expression of TLR4 and NF-κB P65 proteins, the contents of TNF-α and IL-6 in the SN, and the decrease of the total distance, average speed, duration of motion of the autonomic movement in 8 min, and the TH immunoactivity were reversed (P<0.05, P<0.01). CONCLUSION: EA can improve the behavioral manifestations of PD rats, which may be associated with its functions in down-regulating the abnormal accumulation of pathological α-syn, TLR4/NF-κB signaling, inhibiting activities of microglia and in up-regulating the expression of TH in the SN of midbrain. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/34865329/[Electroacupuncture_improves_behavioral_activities_by_suppressing_neuroinflammation_and_TLR4/NF_κB_signaling_in_substantia_nigra_of_midbrain_in_Parkinson's_disease_rats]_ L2 - https://medlineplus.gov/parkinsonsdisease.html DB - PRIME DP - Unbound Medicine ER -