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Pharmacological Activities and Characterization of Phenolic and Flavonoid Compounds in Methanolic Extract of Euphorbia cuneata Vahl Aerial Parts.
Molecules. 2021 Dec 03; 26(23)M

Abstract

Euphorbia cuneata Vahl. (Euphorbiaceae) is a plant used in folk medicine for the treatment of pain and inflammation, although the biological basis for these effects has not been thoroughly investigated. The goal of this study was to investigate the pharmacological properties and characterization of phenolic and flavonoid compounds present in the aerial parts of E. cuneata. E. cuneata aerial parts were tested for antioxidant activity (DPPH), antibacterial activity, cell viability and cytotoxic effects, and anti-inflammatory activity. Phenolic and flavonoid contents (HPLC), and volatile constituents (GC-MS) were also characterized. The methanol extract had the highest antioxidant activity, while the ether extract had the lowest. The antioxidant activity of E. cuneata extract increased from (21.11%) at a concentration of 10 µg/mL to (95.53%) at a concentration of 1280 µg/mL. S. aureus was the most sensitive organism with the highest zone of inhibition and lowest MIC, with acetone extract; whereas C. tropicalis was the most resistant, with the lowest inhibition zone. MTT assay revealed that the methanol extract of E. cuneata had significant cytotoxic effects on the A549, Caco-2, and MDA-MB-231 cell lines, respectively. Lower concentrations of methanolic extract gave anti-inflammatory activity, and those effects were compared with indomethacin as a positive control. Pyrogallol was the most abundant phenolic acid, followed by caffeic, p-coumaric, ferulic, syringic, and gallic acids, respectively. The 7-hydroxyflavone and rutin flavonoids were also found in the extract. GC-mass analysis showed that aerial parts of E. cuneata were rich in methyl 12-hydroxy-9-octadecenoate. The volatile components were also composed of considerable amounts of hexadecanoic acid, methyl ester, (9E,12E)-octadeca-9,12-dienoyl chloride, and methyl octadeca-9,12-dienoate as well as a little amount of hexanal dimethyl acetal. It can be concluded that methanolic extract of E. cuneata could be used as an available source of natural bioactive constituents with consequent health benefits.

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Authors+Show Affiliations

Soliman MSM
Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USC), Sadat City 32897, Egypt.
Abdella A
Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USC), Sadat City 32897, Egypt.
Khidr YA
Department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USC), Sadat City 32897, Egypt.
Hassan GOO
Medicinal and Aromatic Plants Department, Natural Products Unit, Desert Research Center, 1 Mathaf El-Matarya St., El-Matareya, Cairo 11753, Egypt.
Al-Saman MA
Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USC), Sadat City 32897, Egypt.
Elsanhoty RM
Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USC), Sadat City 32897, Egypt.

MeSH

A549 CellsAnti-Bacterial AgentsAnti-Inflammatory AgentsAntineoplastic AgentsAntioxidantsCaco-2 CellsEuphorbiaFlavonoidsHumansMethanolPhenolsPlant Extracts

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34885927

Citation

Soliman, Mohamed S M., et al. "Pharmacological Activities and Characterization of Phenolic and Flavonoid Compounds in Methanolic Extract of Euphorbia Cuneata Vahl Aerial Parts." Molecules (Basel, Switzerland), vol. 26, no. 23, 2021.
Soliman MSM, Abdella A, Khidr YA, et al. Pharmacological Activities and Characterization of Phenolic and Flavonoid Compounds in Methanolic Extract of Euphorbia cuneata Vahl Aerial Parts. Molecules. 2021;26(23).
Soliman, M. S. M., Abdella, A., Khidr, Y. A., Hassan, G. O. O., Al-Saman, M. A., & Elsanhoty, R. M. (2021). Pharmacological Activities and Characterization of Phenolic and Flavonoid Compounds in Methanolic Extract of Euphorbia cuneata Vahl Aerial Parts. Molecules (Basel, Switzerland), 26(23). https://doi.org/10.3390/molecules26237345
Soliman MSM, et al. Pharmacological Activities and Characterization of Phenolic and Flavonoid Compounds in Methanolic Extract of Euphorbia Cuneata Vahl Aerial Parts. Molecules. 2021 Dec 3;26(23) PubMed PMID: 34885927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pharmacological Activities and Characterization of Phenolic and Flavonoid Compounds in Methanolic Extract of Euphorbia cuneata Vahl Aerial Parts. AU - Soliman,Mohamed S M, AU - Abdella,Asmaa, AU - Khidr,Yehia A, AU - Hassan,Gamal O O, AU - Al-Saman,Mahmoud A, AU - Elsanhoty,Rafaat M, Y1 - 2021/12/03/ PY - 2021/10/30/received PY - 2021/11/27/revised PY - 2021/11/29/accepted PY - 2021/12/10/entrez PY - 2021/12/11/pubmed PY - 2022/2/11/medline KW - Euphorbia cuneata Vahl. KW - anti-inflammatory KW - antibacterial KW - antioxidant KW - cell lines KW - cytotoxicity KW - flavonoids KW - phenolics JF - Molecules (Basel, Switzerland) JO - Molecules VL - 26 IS - 23 N2 - Euphorbia cuneata Vahl. (Euphorbiaceae) is a plant used in folk medicine for the treatment of pain and inflammation, although the biological basis for these effects has not been thoroughly investigated. The goal of this study was to investigate the pharmacological properties and characterization of phenolic and flavonoid compounds present in the aerial parts of E. cuneata. E. cuneata aerial parts were tested for antioxidant activity (DPPH), antibacterial activity, cell viability and cytotoxic effects, and anti-inflammatory activity. Phenolic and flavonoid contents (HPLC), and volatile constituents (GC-MS) were also characterized. The methanol extract had the highest antioxidant activity, while the ether extract had the lowest. The antioxidant activity of E. cuneata extract increased from (21.11%) at a concentration of 10 µg/mL to (95.53%) at a concentration of 1280 µg/mL. S. aureus was the most sensitive organism with the highest zone of inhibition and lowest MIC, with acetone extract; whereas C. tropicalis was the most resistant, with the lowest inhibition zone. MTT assay revealed that the methanol extract of E. cuneata had significant cytotoxic effects on the A549, Caco-2, and MDA-MB-231 cell lines, respectively. Lower concentrations of methanolic extract gave anti-inflammatory activity, and those effects were compared with indomethacin as a positive control. Pyrogallol was the most abundant phenolic acid, followed by caffeic, p-coumaric, ferulic, syringic, and gallic acids, respectively. The 7-hydroxyflavone and rutin flavonoids were also found in the extract. GC-mass analysis showed that aerial parts of E. cuneata were rich in methyl 12-hydroxy-9-octadecenoate. The volatile components were also composed of considerable amounts of hexadecanoic acid, methyl ester, (9E,12E)-octadeca-9,12-dienoyl chloride, and methyl octadeca-9,12-dienoate as well as a little amount of hexanal dimethyl acetal. It can be concluded that methanolic extract of E. cuneata could be used as an available source of natural bioactive constituents with consequent health benefits. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/34885927/Pharmacological_Activities_and_Characterization_of_Phenolic_and_Flavonoid_Compounds_in_Methanolic_Extract_of_Euphorbia_cuneata_Vahl_Aerial_Parts_ DB - PRIME DP - Unbound Medicine ER -