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HLA-DP can be expressed with or without -DR molecules on a malignant B cell line.
J Immunol. 1986 Sep 15; 137(6):1932-6.JI

Abstract

HLA class II molecules (HLA-DR, -DQ, -DP) appear to differ in their ability to serve as corecognition elements in antigen presentation to T lymphocytes. Expression of these molecules on the autologous malignant B cell lines BALM-3, -4, and -5 was studied by binding of alloantisera and by indirect immunofluorescence and immune precipitation performed with well-characterized monoclonal antibodies. The following phenotypes were identified: BALM-5, HLA-DR+, -DP+; BALM-4, HLA-DR-, -DP+; BALM-3, HLA-DR-, -DP-. When treated with phorbol acetate (TPA), all three cell lines synthesize and express both HLA-DR and -DP molecules, indicating that the structural genes for these molecules remain intact. Thus HLA-DP can be expressed with or without HLA-DR molecules. Immune precipitation studies of metabolically labeled BALM-4 cells detect HLA-DR molecules in cells from TPA-treated but not control cultures, suggesting that TPA acts by inducing the transcription and/or translation of the genes for these class II molecules. HLA-DP molecules are detected in cells from both BALM-4 cultures. Recent studies suggest that BALM-5 but not BALM-3 or BALM-4 cells are HLA-DQ positive. TPA also appears to induce expression of HLA-DQ molecules on the latter two cell lines. The unique class II phenotypes of these autologous B cell lines in the resting state therefore appear to reflect differences in their ability to execute discrete steps leading to the surface expression of individual class II molecules. Variable expression of individual class II molecules by different cell populations may affect their ability to present cell-associated antigens to T lymphocytes.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3489045

Citation

Pesando, J M., et al. "HLA-DP Can Be Expressed With or Without -DR Molecules On a Malignant B Cell Line." Journal of Immunology (Baltimore, Md. : 1950), vol. 137, no. 6, 1986, pp. 1932-6.
Pesando JM, Graf L, Hoffman P. HLA-DP can be expressed with or without -DR molecules on a malignant B cell line. J Immunol. 1986;137(6):1932-6.
Pesando, J. M., Graf, L., & Hoffman, P. (1986). HLA-DP can be expressed with or without -DR molecules on a malignant B cell line. Journal of Immunology (Baltimore, Md. : 1950), 137(6), 1932-6.
Pesando JM, Graf L, Hoffman P. HLA-DP Can Be Expressed With or Without -DR Molecules On a Malignant B Cell Line. J Immunol. 1986 Sep 15;137(6):1932-6. PubMed PMID: 3489045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HLA-DP can be expressed with or without -DR molecules on a malignant B cell line. AU - Pesando,J M, AU - Graf,L, AU - Hoffman,P, PY - 1986/9/15/pubmed PY - 1986/9/15/medline PY - 1986/9/15/entrez SP - 1932 EP - 6 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 137 IS - 6 N2 - HLA class II molecules (HLA-DR, -DQ, -DP) appear to differ in their ability to serve as corecognition elements in antigen presentation to T lymphocytes. Expression of these molecules on the autologous malignant B cell lines BALM-3, -4, and -5 was studied by binding of alloantisera and by indirect immunofluorescence and immune precipitation performed with well-characterized monoclonal antibodies. The following phenotypes were identified: BALM-5, HLA-DR+, -DP+; BALM-4, HLA-DR-, -DP+; BALM-3, HLA-DR-, -DP-. When treated with phorbol acetate (TPA), all three cell lines synthesize and express both HLA-DR and -DP molecules, indicating that the structural genes for these molecules remain intact. Thus HLA-DP can be expressed with or without HLA-DR molecules. Immune precipitation studies of metabolically labeled BALM-4 cells detect HLA-DR molecules in cells from TPA-treated but not control cultures, suggesting that TPA acts by inducing the transcription and/or translation of the genes for these class II molecules. HLA-DP molecules are detected in cells from both BALM-4 cultures. Recent studies suggest that BALM-5 but not BALM-3 or BALM-4 cells are HLA-DQ positive. TPA also appears to induce expression of HLA-DQ molecules on the latter two cell lines. The unique class II phenotypes of these autologous B cell lines in the resting state therefore appear to reflect differences in their ability to execute discrete steps leading to the surface expression of individual class II molecules. Variable expression of individual class II molecules by different cell populations may affect their ability to present cell-associated antigens to T lymphocytes. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/3489045/HLA_DP_can_be_expressed_with_or_without__DR_molecules_on_a_malignant_B_cell_line_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=3489045 DB - PRIME DP - Unbound Medicine ER -