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Multiplex polymerase chain reaction detection of Streptococcus pneumoniae and Haemophilus influenzae and their antibiotic resistance in patients with community-acquired pneumonia from southwest Iran.
BMC Microbiol. 2021 12 14; 21(1):343.BM

Abstract

BACKGROUND

This study aimed to evaluate the occurrence of Streptococcus pneumoniae and Haemophilus influenzae in sputum of patients with community-acquired pneumonia (CAP) using culture and multiplex polymerase chain reaction (M-PCR) methods and to survey the antibiotic resistance patterns of aforesaid isolates.

RESULT

In total, 23.9 % (n = 22/92) of sputum samples showed positive results in the culture method. S. pneumoniae and H. influenzae were isolated from 15 (16.3 %) and 7 (7.6%) samples, respectively. Using M-PCR, 44 (47.8 %) samples were positive for S. pneumoniae and H. influenzae. Of these, S. pneumoniae and H. influenzae were detected in 33 (35.8%) and 11 (11.9%) of the sputum samples, respectively. The sensitivity, specificity, and accuracy rates of PCR in detection of S. pneumoniae in comparison with culture method were 100, 76.6, and 83.6%, respectively. While, the sensitivity, specificity, and accuracy rates of PCR in detection of H. influenzae in comparison with culture method were 100, 95.3, and 95.8%, respectively. Out of 11 isolates of H. influenzae, two strains confirmed as H. influenzae type b (Hib) and 3 isolates were type f. However, 6 isolates were non-typable. The co-trimoxazole and amoxicillin/clavulanate were the less effective antibiotics against S. pneumonia and H. influenzae, respectively. Ceftriaxone with 13.3% resistance rates was the most effective antibiotic against S. pneumoniae, while, clarithromycin, ceftriaxone, and gentamicin with resistance rates of 28.6% for each one were the most effective chemicals against H. influenzae isolates.

CONCLUSION

In this study, the prevalence of S. pneumoniae was more than H. influenzae using culture and M-PCR methods. The M-PCR provided better efficiency in detecting the bacterial agents in CAP patients compared to culture method. This method can improve the early detection of pathogens contributed to CAP. The drug resistant S. pneumoniae and H. influenzae indicated the need to develop a codified monitoring program to prevent further spread of these strains.

Authors+Show Affiliations

Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. rahimirobab@gmail.com. Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. rahimirobab@gmail.com.Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. H.meghdadi20@gmail.com. Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. H.meghdadi20@gmail.com. Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. H.meghdadi20@gmail.com.Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. mortezasaki1981@gmail.com. Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. mortezasaki1981@gmail.com. Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. mortezasaki1981@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

34906085

Citation

Farajzadeh Sheikh, Ahmad, et al. "Multiplex Polymerase Chain Reaction Detection of Streptococcus Pneumoniae and Haemophilus Influenzae and Their Antibiotic Resistance in Patients With Community-acquired Pneumonia From Southwest Iran." BMC Microbiology, vol. 21, no. 1, 2021, p. 343.
Farajzadeh Sheikh A, Rahimi R, Meghdadi H, et al. Multiplex polymerase chain reaction detection of Streptococcus pneumoniae and Haemophilus influenzae and their antibiotic resistance in patients with community-acquired pneumonia from southwest Iran. BMC Microbiol. 2021;21(1):343.
Farajzadeh Sheikh, A., Rahimi, R., Meghdadi, H., Alami, A., & Saki, M. (2021). Multiplex polymerase chain reaction detection of Streptococcus pneumoniae and Haemophilus influenzae and their antibiotic resistance in patients with community-acquired pneumonia from southwest Iran. BMC Microbiology, 21(1), 343. https://doi.org/10.1186/s12866-021-02408-7
Farajzadeh Sheikh A, et al. Multiplex Polymerase Chain Reaction Detection of Streptococcus Pneumoniae and Haemophilus Influenzae and Their Antibiotic Resistance in Patients With Community-acquired Pneumonia From Southwest Iran. BMC Microbiol. 2021 12 14;21(1):343. PubMed PMID: 34906085.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiplex polymerase chain reaction detection of Streptococcus pneumoniae and Haemophilus influenzae and their antibiotic resistance in patients with community-acquired pneumonia from southwest Iran. AU - Farajzadeh Sheikh,Ahmad, AU - Rahimi,Robab, AU - Meghdadi,Hossein, AU - Alami,Ameneh, AU - Saki,Morteza, Y1 - 2021/12/14/ PY - 2021/08/10/received PY - 2021/12/02/accepted PY - 2021/12/15/entrez PY - 2021/12/16/pubmed PY - 2022/1/28/medline KW - CAP KW - Community-acquired pneumonia KW - Haemophilus influenzae KW - Multiplex polymerase chain reaction KW - Streptococcus pneumoniae SP - 343 EP - 343 JF - BMC microbiology JO - BMC Microbiol VL - 21 IS - 1 N2 - BACKGROUND: This study aimed to evaluate the occurrence of Streptococcus pneumoniae and Haemophilus influenzae in sputum of patients with community-acquired pneumonia (CAP) using culture and multiplex polymerase chain reaction (M-PCR) methods and to survey the antibiotic resistance patterns of aforesaid isolates. RESULT: In total, 23.9 % (n = 22/92) of sputum samples showed positive results in the culture method. S. pneumoniae and H. influenzae were isolated from 15 (16.3 %) and 7 (7.6%) samples, respectively. Using M-PCR, 44 (47.8 %) samples were positive for S. pneumoniae and H. influenzae. Of these, S. pneumoniae and H. influenzae were detected in 33 (35.8%) and 11 (11.9%) of the sputum samples, respectively. The sensitivity, specificity, and accuracy rates of PCR in detection of S. pneumoniae in comparison with culture method were 100, 76.6, and 83.6%, respectively. While, the sensitivity, specificity, and accuracy rates of PCR in detection of H. influenzae in comparison with culture method were 100, 95.3, and 95.8%, respectively. Out of 11 isolates of H. influenzae, two strains confirmed as H. influenzae type b (Hib) and 3 isolates were type f. However, 6 isolates were non-typable. The co-trimoxazole and amoxicillin/clavulanate were the less effective antibiotics against S. pneumonia and H. influenzae, respectively. Ceftriaxone with 13.3% resistance rates was the most effective antibiotic against S. pneumoniae, while, clarithromycin, ceftriaxone, and gentamicin with resistance rates of 28.6% for each one were the most effective chemicals against H. influenzae isolates. CONCLUSION: In this study, the prevalence of S. pneumoniae was more than H. influenzae using culture and M-PCR methods. The M-PCR provided better efficiency in detecting the bacterial agents in CAP patients compared to culture method. This method can improve the early detection of pathogens contributed to CAP. The drug resistant S. pneumoniae and H. influenzae indicated the need to develop a codified monitoring program to prevent further spread of these strains. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/34906085/Multiplex_polymerase_chain_reaction_detection_of_Streptococcus_pneumoniae_and_Haemophilus_influenzae_and_their_antibiotic_resistance_in_patients_with_community_acquired_pneumonia_from_southwest_Iran_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-021-02408-7 DB - PRIME DP - Unbound Medicine ER -