Tags

Type your tag names separated by a space and hit enter

Optimization Method for Phenolic Compounds Extraction from Medicinal Plant (Juniperus procera) and Phytochemicals Screening.
Molecules. 2021 Dec 09; 26(24)M

Abstract

Juniperus procera is a natural source of bioactive compounds with the potential of antitumor, antimicrobial, insecticidal, antifungal, and antioxidant activities. An optimization method was developed for total phenolic content (TPC), total flavonoid content (TFC), and total tannin content (TTC) in leaf and seed extract of Juniperus procera. Organic solvents (methanol (99.8%), ethanol (99%), and acetone (99.5%)), and deionized water (DI) were used for extraction. The estimation of TPC, TFC, and TTC in plant materials was carried out using UV-spectrophotometer and HPLC with the standards gallic acid, quercetin, and tannic acid. Recovery of TPC in leaf extract ranged from 2.9 to 9.7 mg GAE/g DW, TFC from 0.9 to 5.9 mg QE/g DW, and TTC ranged from 1.5 to 4.3 mg TA/g DW while the TPC value in the seed extract ranged from 0.53 to 2.6 mg GAE/g DW, TFC from 0.5 to 1.6 mg QE/g DW, and TTC ranged from 0.5 to 1.4 mg TA/g DW. This result revealed that methanol is the best solvent for recovery of the TPC value (9.7 mg) from leaf extract in comparison to other solvents. Ethanol recorded the highest result of TFC (5.9 mg) in leaf extract among the solvents whereas acetone was the best for TTC yield recovery from leaf extract (4.3 mg). In the case of the seed extract, ethanol was the best solvent for both TPC (2.6 mg), and TFC (1.6 mg) recovery in comparison to other solvents. Total tannin content in methanol resulted in significant recovery from seed extract (1.4 mg). Separation and quantification of gallic acid, quercetin, and tannic acid in plant materials were undertaken using HPLC. Gallic acid in leaf and seed of J. procera ranged from 6.6 to 9.2, 6.5 to 7.2 µg/g DW, quercetin from 6.3 to 18.2, 0.9 to 4.2 µg/g DW, and tannic acid from 16.2 to 29.3, 6.6 to 9.3 µg/g DW, respectively. Solvents have shown a significant effect in the extraction of phenolic compounds. Moreover, phytochemicals in plant materials were identified using GC-MS and resulted in very important bioactive compounds, which include anti-inflammatory, antibacterial, and antitumor agents such as ferruginol, phenanthrene, and n-hexadecanoic acid. In conclusion, the optimal solvent for extraction depends on the part of the plant material and the compounds that are to be isolated.

Links

Authors+Show Affiliations

Botany and Microbiology Department, College of Science King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

Botany and Microbiology Department, College of Science King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

Botany and Microbiology Department, College of Science King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

Botany and Microbiology Department, College of Science King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

Botany and Microbiology Department, College of Science King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

Shaikhaldein HO

Botany and Microbiology Department, College of Science King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

Botany and Microbiology Department, College of Science King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

Botany and Microbiology Department, College of Science King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

Botany and Microbiology Department, College of Science King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

MeSH

AntioxidantsFlavonoidsJuniperusPhenolsPhytochemicalsPlant ExtractsPlants, Medicinal

Pub Type(s)

Journal Article

Language

eng

PubMed ID

34946537

Citation

Salih, Abdalrhaman M., et al. "Optimization Method for Phenolic Compounds Extraction From Medicinal Plant (Juniperus Procera) and Phytochemicals Screening." Molecules (Basel, Switzerland), vol. 26, no. 24, 2021.

Salih AM, Al-Qurainy F, Nadeem M, et al. Optimization Method for Phenolic Compounds Extraction from Medicinal Plant (Juniperus procera) and Phytochemicals Screening. Molecules. 2021;26(24).

Salih, A. M., Al-Qurainy, F., Nadeem, M., Tarroum, M., Khan, S., Shaikhaldein, H. O., Al-Hashimi, A., Alfagham, A., & Alkahtani, J. (2021). Optimization Method for Phenolic Compounds Extraction from Medicinal Plant (Juniperus procera) and Phytochemicals Screening. Molecules (Basel, Switzerland), 26(24). https://doi.org/10.3390/molecules26247454

Salih AM, et al. Optimization Method for Phenolic Compounds Extraction From Medicinal Plant (Juniperus Procera) and Phytochemicals Screening. Molecules. 2021 Dec 9;26(24) PubMed PMID: 34946537.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Optimization Method for Phenolic Compounds Extraction from Medicinal Plant (Juniperus procera) and Phytochemicals Screening. AU - Salih,Abdalrhaman M, AU - Al-Qurainy,Fahad, AU - Nadeem,Mohammad, AU - Tarroum,Mohamed, AU - Khan,Salim, AU - Shaikhaldein,Hassan O, AU - Al-Hashimi,Abdulrahman, AU - Alfagham,Alanoud, AU - Alkahtani,Jawaher, Y1 - 2021/12/09/ PY - 2021/11/22/received PY - 2021/12/05/revised PY - 2021/12/07/accepted PY - 2021/12/24/entrez PY - 2021/12/25/pubmed PY - 2022/2/12/medline KW - chromatography analysis KW - flavonoid KW - new approach KW - phytochemicals profiling KW - solvents KW - spectrophotometry KW - tannin JF - Molecules (Basel, Switzerland) JO - Molecules VL - 26 IS - 24 N2 - Juniperus procera is a natural source of bioactive compounds with the potential of antitumor, antimicrobial, insecticidal, antifungal, and antioxidant activities. An optimization method was developed for total phenolic content (TPC), total flavonoid content (TFC), and total tannin content (TTC) in leaf and seed extract of Juniperus procera. Organic solvents (methanol (99.8%), ethanol (99%), and acetone (99.5%)), and deionized water (DI) were used for extraction. The estimation of TPC, TFC, and TTC in plant materials was carried out using UV-spectrophotometer and HPLC with the standards gallic acid, quercetin, and tannic acid. Recovery of TPC in leaf extract ranged from 2.9 to 9.7 mg GAE/g DW, TFC from 0.9 to 5.9 mg QE/g DW, and TTC ranged from 1.5 to 4.3 mg TA/g DW while the TPC value in the seed extract ranged from 0.53 to 2.6 mg GAE/g DW, TFC from 0.5 to 1.6 mg QE/g DW, and TTC ranged from 0.5 to 1.4 mg TA/g DW. This result revealed that methanol is the best solvent for recovery of the TPC value (9.7 mg) from leaf extract in comparison to other solvents. Ethanol recorded the highest result of TFC (5.9 mg) in leaf extract among the solvents whereas acetone was the best for TTC yield recovery from leaf extract (4.3 mg). In the case of the seed extract, ethanol was the best solvent for both TPC (2.6 mg), and TFC (1.6 mg) recovery in comparison to other solvents. Total tannin content in methanol resulted in significant recovery from seed extract (1.4 mg). Separation and quantification of gallic acid, quercetin, and tannic acid in plant materials were undertaken using HPLC. Gallic acid in leaf and seed of J. procera ranged from 6.6 to 9.2, 6.5 to 7.2 µg/g DW, quercetin from 6.3 to 18.2, 0.9 to 4.2 µg/g DW, and tannic acid from 16.2 to 29.3, 6.6 to 9.3 µg/g DW, respectively. Solvents have shown a significant effect in the extraction of phenolic compounds. Moreover, phytochemicals in plant materials were identified using GC-MS and resulted in very important bioactive compounds, which include anti-inflammatory, antibacterial, and antitumor agents such as ferruginol, phenanthrene, and n-hexadecanoic acid. In conclusion, the optimal solvent for extraction depends on the part of the plant material and the compounds that are to be isolated. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/34946537/Optimization_Method_for_Phenolic_Compounds_Extraction_from_Medicinal_Plant__Juniperus_procera__and_Phytochemicals_Screening_ DB - PRIME DP - Unbound Medicine ER -