Tags

Type your tag names separated by a space and hit enter

Phenotypically cryptic EcoRI endonuclease activity specified by the ColE1 plasmid.
Proc Natl Acad Sci U S A. 1978 Mar; 75(3):1265-9.PN

Abstract

An endonuclease having EcoRI specificity is produced by bacteria containing the ColE1 plasmid. Such bacterial cells fail to express restriction or modification functions in vivo, and phage or plasmid DNA obtained from ColE1-containing cells has unmodified EcoRI sites that are cleaved in vitro by purified EcoRI endonuclease or by enzyme extracted from bacteria that carry ColE1. No EcoRI DNA methylase activity associated with ColE1 has been detected. The finding of phenotypically cryptic ColE1-dependent EcoRI endonuclease activity and the absence of any detectable EcoRI modification system in ColE1-containing cells suggest a control mechanism that appears to prevent functional expression of the ColE1-determined enzyme in vivo.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

349563

Citation

Miller, C A., and S N. Cohen. "Phenotypically Cryptic EcoRI Endonuclease Activity Specified By the ColE1 Plasmid." Proceedings of the National Academy of Sciences of the United States of America, vol. 75, no. 3, 1978, pp. 1265-9.
Miller CA, Cohen SN. Phenotypically cryptic EcoRI endonuclease activity specified by the ColE1 plasmid. Proc Natl Acad Sci USA. 1978;75(3):1265-9.
Miller, C. A., & Cohen, S. N. (1978). Phenotypically cryptic EcoRI endonuclease activity specified by the ColE1 plasmid. Proceedings of the National Academy of Sciences of the United States of America, 75(3), 1265-9.
Miller CA, Cohen SN. Phenotypically Cryptic EcoRI Endonuclease Activity Specified By the ColE1 Plasmid. Proc Natl Acad Sci USA. 1978;75(3):1265-9. PubMed PMID: 349563.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypically cryptic EcoRI endonuclease activity specified by the ColE1 plasmid. AU - Miller,C A, AU - Cohen,S N, PY - 1978/3/1/pubmed PY - 1978/3/1/medline PY - 1978/3/1/entrez SP - 1265 EP - 9 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 75 IS - 3 N2 - An endonuclease having EcoRI specificity is produced by bacteria containing the ColE1 plasmid. Such bacterial cells fail to express restriction or modification functions in vivo, and phage or plasmid DNA obtained from ColE1-containing cells has unmodified EcoRI sites that are cleaved in vitro by purified EcoRI endonuclease or by enzyme extracted from bacteria that carry ColE1. No EcoRI DNA methylase activity associated with ColE1 has been detected. The finding of phenotypically cryptic ColE1-dependent EcoRI endonuclease activity and the absence of any detectable EcoRI modification system in ColE1-containing cells suggest a control mechanism that appears to prevent functional expression of the ColE1-determined enzyme in vivo. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/349563/Phenotypically_cryptic_EcoRI_endonuclease_activity_specified_by_the_ColE1_plasmid_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/349563/ DB - PRIME DP - Unbound Medicine ER -