Tags

Type your tag names separated by a space and hit enter

T cell regulation of thyroglobulin autoantibody IgG subclasses in Hashimoto's thyroiditis.
Clin Exp Immunol. 1987 Aug; 69(2):314-22.CE

Abstract

Microsomal and thyroglobulin (Tg) antibodies in patients with autoimmune thyroid disease are usually predominantly of subclasses IgG1 and/or IgG4 and the distribution pattern is characteristic for the serum of an individual. We have studied the role of T cells in synthesis of total IgG and Tg antibody IgG subclasses (measured by ELISA) in cultures of peripheral blood lymphocytes (PBL) from Hashimoto patients. Unfractionated PBL incubated with the T dependent activator pokeweed mitogen (PWM) synthesized IgG of all four IgG subclasses in the proportions 69% IgG1, 20% IgG2, 8% IgG3 and 3% IgG4; these values are similar to the proportions of the subclasses in serum. In contrast, the IgG subclass of Tg antibody was predominantly IgG1 in one patient, approximately equal proportions of IgG1 and IgG4 in four patients, and almost completely restricted to IgG4 in one patient; these patterns were similar to the subclass distribution of the autoantibodies in the individual patients' serum. B cells incubated alone secreted little Tg antibody but the response could be restored to the original levels and proportions of IgG1 and/or IgG4 Tg antibody by the addition of T cells either from the same individual or from another donor. Further, removal of suppressor T cells had little effect on the proportions of IgG1 and IgG4 Tg antibody although the total amounts of Tg antibody of both subclasses were sometimes increased. Our studies indicate that T cells are required in this in vitro system to elicit Tg antibody synthesis and to control the magnitude of the antibody response. However, the characteristic IgG subclass distribution of Tg antibody in an individual is determined at the level of the B cell.

Authors+Show Affiliations

Department of Pathology, University of Newcastle upon Tyne, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3498591

Citation

Forouhi, N G., et al. "T Cell Regulation of Thyroglobulin Autoantibody IgG Subclasses in Hashimoto's Thyroiditis." Clinical and Experimental Immunology, vol. 69, no. 2, 1987, pp. 314-22.
Forouhi NG, McLachlan SM, Middleton SL, et al. T cell regulation of thyroglobulin autoantibody IgG subclasses in Hashimoto's thyroiditis. Clin Exp Immunol. 1987;69(2):314-22.
Forouhi, N. G., McLachlan, S. M., Middleton, S. L., Atherton, M. C., Baylis, P., Clark, F., & Smith, B. R. (1987). T cell regulation of thyroglobulin autoantibody IgG subclasses in Hashimoto's thyroiditis. Clinical and Experimental Immunology, 69(2), 314-22.
Forouhi NG, et al. T Cell Regulation of Thyroglobulin Autoantibody IgG Subclasses in Hashimoto's Thyroiditis. Clin Exp Immunol. 1987;69(2):314-22. PubMed PMID: 3498591.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - T cell regulation of thyroglobulin autoantibody IgG subclasses in Hashimoto's thyroiditis. AU - Forouhi,N G, AU - McLachlan,S M, AU - Middleton,S L, AU - Atherton,M C, AU - Baylis,P, AU - Clark,F, AU - Smith,B R, PY - 1987/8/1/pubmed PY - 1987/8/1/medline PY - 1987/8/1/entrez SP - 314 EP - 22 JF - Clinical and experimental immunology JO - Clin. Exp. Immunol. VL - 69 IS - 2 N2 - Microsomal and thyroglobulin (Tg) antibodies in patients with autoimmune thyroid disease are usually predominantly of subclasses IgG1 and/or IgG4 and the distribution pattern is characteristic for the serum of an individual. We have studied the role of T cells in synthesis of total IgG and Tg antibody IgG subclasses (measured by ELISA) in cultures of peripheral blood lymphocytes (PBL) from Hashimoto patients. Unfractionated PBL incubated with the T dependent activator pokeweed mitogen (PWM) synthesized IgG of all four IgG subclasses in the proportions 69% IgG1, 20% IgG2, 8% IgG3 and 3% IgG4; these values are similar to the proportions of the subclasses in serum. In contrast, the IgG subclass of Tg antibody was predominantly IgG1 in one patient, approximately equal proportions of IgG1 and IgG4 in four patients, and almost completely restricted to IgG4 in one patient; these patterns were similar to the subclass distribution of the autoantibodies in the individual patients' serum. B cells incubated alone secreted little Tg antibody but the response could be restored to the original levels and proportions of IgG1 and/or IgG4 Tg antibody by the addition of T cells either from the same individual or from another donor. Further, removal of suppressor T cells had little effect on the proportions of IgG1 and IgG4 Tg antibody although the total amounts of Tg antibody of both subclasses were sometimes increased. Our studies indicate that T cells are required in this in vitro system to elicit Tg antibody synthesis and to control the magnitude of the antibody response. However, the characteristic IgG subclass distribution of Tg antibody in an individual is determined at the level of the B cell. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/3498591/T_cell_regulation_of_thyroglobulin_autoantibody_IgG_subclasses_in_Hashimoto's_thyroiditis_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/3498591/ DB - PRIME DP - Unbound Medicine ER -