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[Use of the plasmid R89S replicon for constructing integration vectors].

Abstract

It has been shown that the plasmid R89S derivatives can be used as integrative vectors for bacteria in which the plasmid is unable to replicate autonomously. The chromosomal and plasmid fragments of phototrophic bacterium Rhodobacter sphaeroides have been cloned in plasmid pVZ365, a SmRKmR-derivative of R89S. The obtained recombinant plasmids were mobilized into R. sphaeroides cells by the I pcP-group conjugative plasmid R751. The frequencies of the SmR-transconjugants formation are 3.7.10(-5) to 5.6.10(-3) per recipient cell. The formation of the SmR-transconjugants has not been revealed in case of the plasmid pVZ365 mobilization. The recombinant molecules containing R. sphaeroides plasmid fragments have been shown to integrate into endogenous plasmids and form cointegrates with them.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

3499569

Citation

Zinchenko, V V., et al. "[Use of the Plasmid R89S Replicon for Constructing Integration Vectors]." Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, 1987, pp. 11-3.
Zinchenko VV, Saano AK, Babykin MM, et al. [Use of the plasmid R89S replicon for constructing integration vectors]. Mol Gen Mikrobiol Virusol. 1987.
Zinchenko, V. V., Saano, A. K., Babykin, M. M., & Shestakov, S. V. (1987). [Use of the plasmid R89S replicon for constructing integration vectors]. Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, (7), 11-3.
Zinchenko VV, et al. [Use of the Plasmid R89S Replicon for Constructing Integration Vectors]. Mol Gen Mikrobiol Virusol. 1987;(7)11-3. PubMed PMID: 3499569.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Use of the plasmid R89S replicon for constructing integration vectors]. AU - Zinchenko,V V, AU - Saano,A K, AU - Babykin,M M, AU - Shestakov,S V, PY - 1987/7/1/pubmed PY - 1987/7/1/medline PY - 1987/7/1/entrez SP - 11 EP - 3 JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia JO - Mol. Gen. Mikrobiol. Virusol. IS - 7 N2 - It has been shown that the plasmid R89S derivatives can be used as integrative vectors for bacteria in which the plasmid is unable to replicate autonomously. The chromosomal and plasmid fragments of phototrophic bacterium Rhodobacter sphaeroides have been cloned in plasmid pVZ365, a SmRKmR-derivative of R89S. The obtained recombinant plasmids were mobilized into R. sphaeroides cells by the I pcP-group conjugative plasmid R751. The frequencies of the SmR-transconjugants formation are 3.7.10(-5) to 5.6.10(-3) per recipient cell. The formation of the SmR-transconjugants has not been revealed in case of the plasmid pVZ365 mobilization. The recombinant molecules containing R. sphaeroides plasmid fragments have been shown to integrate into endogenous plasmids and form cointegrates with them. SN - 0208-0613 UR - https://www.unboundmedicine.com/medline/citation/3499569/[Use_of_the_plasmid_R89S_replicon_for_constructing_integration_vectors]_ DB - PRIME DP - Unbound Medicine ER -
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