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Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array.
Front Plant Sci. 2021; 12:783830.FP

Abstract

Stripe rust (caused by Puccinia striiformis f. sp. tritici) is one of the most severe diseases affecting wheat production. The disease is best controlled by developing and growing resistant cultivars. Chinese wheat (Triticum aestivum) landraces have excellent resistance to stripe rust. The objectives of this study were to identify wheat landraces with stable resistance and map quantitative trait loci (QTL) for resistance to stripe rust from 271 Chinese wheat landraces using a genome-wide association study (GWAS) approach. The landraces were phenotyped for stripe rust responses at the seedling stage with two predominant Chinese races of P. striiformis f. sp. tritici in a greenhouse and the adult-plant stage in four field environments and genotyped using the 660K wheat single-nucleotide polymorphism (SNP) array. Thirteen landraces with stable resistance were identified, and 17 QTL, including eight associated to all-stage resistance and nine to adult-plant resistance, were mapped on chromosomes 1A, 1B, 2A, 2D, 3A, 3B, 5A, 5B, 6D, and 7A. These QTL explained 6.06-16.46% of the phenotypic variation. Five of the QTL, QYrCL.sicau-3AL, QYrCL.sicau-3B.4, QYrCL.sicau-3B.5, QYrCL.sicau-5AL.1 and QYrCL.sicau-7AL, were likely new. Five Kompetitive allele specific PCR (KASP) markers for four of the QTL were converted from the significant SNP markers. The identified wheat landraces with stable resistance to stripe rust, significant QTL, and KASP markers should be useful for breeding wheat cultivars with durable resistance to stripe rust.

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Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.

State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. College of Agronomy, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.

College of Agronomy, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

College of Agronomy, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.

Wheat Health, Genetics and Quality Research Unit, United States Department of Agriculture, Agricultural Research Service, Pullman, WA, United States. Department of Plant Pathology, Washington State University, Pullman, WA, United States.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35003168

Citation

Yao, Fangjie, et al. "Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array." Frontiers in Plant Science, vol. 12, 2021, p. 783830.

Yao F, Guan F, Duan L, et al. Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array. Front Plant Sci. 2021;12:783830.

Yao, F., Guan, F., Duan, L., Long, L., Tang, H., Jiang, Y., Li, H., Jiang, Q., Wang, J., Qi, P., Kang, H., Li, W., Ma, J., Pu, Z., Deng, M., Wei, Y., Zheng, Y., Chen, X., & Chen, G. (2021). Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array. Frontiers in Plant Science, 12, 783830. https://doi.org/10.3389/fpls.2021.783830

Yao F, et al. Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array. Front Plant Sci. 2021;12:783830. PubMed PMID: 35003168.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array. AU - Yao,Fangjie, AU - Guan,Fangnian, AU - Duan,Luyao, AU - Long,Li, AU - Tang,Hao, AU - Jiang,Yunfeng, AU - Li,Hao, AU - Jiang,Qiantao, AU - Wang,Jirui, AU - Qi,Pengfei, AU - Kang,Houyang, AU - Li,Wei, AU - Ma,Jian, AU - Pu,Zhien, AU - Deng,Mei, AU - Wei,Yuming, AU - Zheng,Youliang, AU - Chen,Xianming, AU - Chen,Guoyue, Y1 - 2021/12/22/ PY - 2021/09/27/received PY - 2021/11/23/accepted PY - 2022/1/10/entrez PY - 2022/1/11/pubmed PY - 2022/1/11/medline KW - GWAS KW - KASP markers KW - resistance KW - stripe rust KW - wheat landraces SP - 783830 EP - 783830 JF - Frontiers in plant science JO - Front Plant Sci VL - 12 N2 - Stripe rust (caused by Puccinia striiformis f. sp. tritici) is one of the most severe diseases affecting wheat production. The disease is best controlled by developing and growing resistant cultivars. Chinese wheat (Triticum aestivum) landraces have excellent resistance to stripe rust. The objectives of this study were to identify wheat landraces with stable resistance and map quantitative trait loci (QTL) for resistance to stripe rust from 271 Chinese wheat landraces using a genome-wide association study (GWAS) approach. The landraces were phenotyped for stripe rust responses at the seedling stage with two predominant Chinese races of P. striiformis f. sp. tritici in a greenhouse and the adult-plant stage in four field environments and genotyped using the 660K wheat single-nucleotide polymorphism (SNP) array. Thirteen landraces with stable resistance were identified, and 17 QTL, including eight associated to all-stage resistance and nine to adult-plant resistance, were mapped on chromosomes 1A, 1B, 2A, 2D, 3A, 3B, 5A, 5B, 6D, and 7A. These QTL explained 6.06-16.46% of the phenotypic variation. Five of the QTL, QYrCL.sicau-3AL, QYrCL.sicau-3B.4, QYrCL.sicau-3B.5, QYrCL.sicau-5AL.1 and QYrCL.sicau-7AL, were likely new. Five Kompetitive allele specific PCR (KASP) markers for four of the QTL were converted from the significant SNP markers. The identified wheat landraces with stable resistance to stripe rust, significant QTL, and KASP markers should be useful for breeding wheat cultivars with durable resistance to stripe rust. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/35003168/Genome_Wide_Association_Analysis_of_Stable_Stripe_Rust_Resistance_Loci_in_a_Chinese_Wheat_Landrace_Panel_Using_the_660K_SNP_Array_ DB - PRIME DP - Unbound Medicine ER -