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Analytical Strategies for LC-MS-Based Untargeted and Targeted Metabolomics Approaches Reveal the Entomological Origins of Honey.
J Agric Food Chem. 2022 Feb 02; 70(4):1358-1366.JA

Abstract

A comprehensive liquid chromatography-mass spectrometry (LC-MS)-based metabolomics approach was developed to discriminate honey harvested from Apis mellifera ligustica Spinola (A. mellifera) and Apis cerana cerana Fabricius (A. cerana). Based on an untargeted strategy, ultrahigh-performance liquid chromatography electrospray ionization quadrupole orbitrap high-resolution mass spectrometry (UPLC Q-Orbitrap) was combined with chemometrics techniques to screen and identify tentative markers from A. mellifera and A. cerana honey. In targeted metabolomics analysis, a sensitive method of solid-phase extraction followed by ultrahigh-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UPLC-MS/MS) was established for quantifying three markers, and the results showed that 3-amino-2-naphthoic acid and methyl indole-3-acetate could be considered markers of A. cerana honey, as they were present in higher amounts in A. cerana honey than in A. mellifera honey, whereas kynurenic acid was determined to be a marker of A. mellifera honey. This work highlights critical information for the authentication of A. cerana and A. mellifera honey.

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Authors+Show Affiliations

Wang X
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, P. R. China.
Li Y
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China.
Chen L
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, P. R. China. Laboratory of Risk Assessment for Quality and Safety of Bee Products, Ministry of Agriculture and Rural Affairs, Beijing 100093, P. R. China. Key Laboratory of Bee Products for Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Beijing 100093, P. R. China.
Zhou J
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, P. R. China. Laboratory of Risk Assessment for Quality and Safety of Bee Products, Ministry of Agriculture and Rural Affairs, Beijing 100093, P. R. China. Key Laboratory of Bee Products for Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Beijing 100093, P. R. China.

MeSH

AnimalsBeesChromatography, LiquidHoneyMetabolomicsSolid Phase ExtractionTandem Mass Spectrometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35023735

Citation

Wang, Xinran, et al. "Analytical Strategies for LC-MS-Based Untargeted and Targeted Metabolomics Approaches Reveal the Entomological Origins of Honey." Journal of Agricultural and Food Chemistry, vol. 70, no. 4, 2022, pp. 1358-1366.
Wang X, Li Y, Chen L, et al. Analytical Strategies for LC-MS-Based Untargeted and Targeted Metabolomics Approaches Reveal the Entomological Origins of Honey. J Agric Food Chem. 2022;70(4):1358-1366.
Wang, X., Li, Y., Chen, L., & Zhou, J. (2022). Analytical Strategies for LC-MS-Based Untargeted and Targeted Metabolomics Approaches Reveal the Entomological Origins of Honey. Journal of Agricultural and Food Chemistry, 70(4), 1358-1366. https://doi.org/10.1021/acs.jafc.1c07153
Wang X, et al. Analytical Strategies for LC-MS-Based Untargeted and Targeted Metabolomics Approaches Reveal the Entomological Origins of Honey. J Agric Food Chem. 2022 Feb 2;70(4):1358-1366. PubMed PMID: 35023735.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analytical Strategies for LC-MS-Based Untargeted and Targeted Metabolomics Approaches Reveal the Entomological Origins of Honey. AU - Wang,Xinran, AU - Li,Yi, AU - Chen,Lanzhen, AU - Zhou,Jinhui, Y1 - 2022/01/13/ PY - 2022/1/14/pubmed PY - 2022/2/4/medline PY - 2022/1/13/entrez KW - A. mellifera and A. cerana KW - UPLC Q-Orbitrap KW - UPLC-MS/MS KW - honey KW - untargeted and targeted discrimination SP - 1358 EP - 1366 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 70 IS - 4 N2 - A comprehensive liquid chromatography-mass spectrometry (LC-MS)-based metabolomics approach was developed to discriminate honey harvested from Apis mellifera ligustica Spinola (A. mellifera) and Apis cerana cerana Fabricius (A. cerana). Based on an untargeted strategy, ultrahigh-performance liquid chromatography electrospray ionization quadrupole orbitrap high-resolution mass spectrometry (UPLC Q-Orbitrap) was combined with chemometrics techniques to screen and identify tentative markers from A. mellifera and A. cerana honey. In targeted metabolomics analysis, a sensitive method of solid-phase extraction followed by ultrahigh-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UPLC-MS/MS) was established for quantifying three markers, and the results showed that 3-amino-2-naphthoic acid and methyl indole-3-acetate could be considered markers of A. cerana honey, as they were present in higher amounts in A. cerana honey than in A. mellifera honey, whereas kynurenic acid was determined to be a marker of A. mellifera honey. This work highlights critical information for the authentication of A. cerana and A. mellifera honey. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/35023735/Analytical_Strategies_for_LC_MS_Based_Untargeted_and_Targeted_Metabolomics_Approaches_Reveal_the_Entomological_Origins_of_Honey_ DB - PRIME DP - Unbound Medicine ER -