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Biogenic Synthesis, Characterization and Antibacterial Properties of Silver Nanoparticles against Human Pathogens.
J Oleo Sci. 2022 Feb 03; 71(2):257-265.JO

Abstract

Biogenic synthesis of silver nanoparticles (AgNPs) is more eco-friendly and cost-effective approach as compared to the conventional chemical synthesis. Biologically synthesized AgNPs have been proved as therapeutically effective and valuable compounds. In this study, the four bacterial strains Escherichia coli (MT448673), Pseudomonas aeruginosa (MN900691), Bacillus subtilis (MN900684) and Bacillus licheniformis (MN900686) were used for the biogenic synthesis of AgNPs. Agar well diffusion assay revealed to determine the antibacterial activity of all biogenically synthesized AGNPs showed that P. aeruginosa AgNPs possessed significantly high (p < 0.05) antibacterial potential against all tested isolates. The one-way ANOVA test showed that that P. aeruginosa AgNPs showed significantly (p < 0.05) larger zones of inhibition (ZOI: 19 to 22 mm) compared to the positive control (rifampicin: 50 µg/mL) while no ZOI was observed against negative control (Dimethyl sulfoxide: DMSO). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) concentration against four test strains also showed that among all biogenically synthesized NPs, P. aeruginosa AgNPs showed effective MIC (3.3-3.6 µg/mL) and MBC (4.3-4.6 µg/mL). Hence, P. aeruginosa AGNPs were characterized using visual UV vis-spectroscopy, X-ray diffractometer (XRD), fourier transform infrared (FTIR) and scanning electron microscopy (SEM). The formation of peak around 430 nm indicated the formation of AgNPs while the FTIR confirmed the involvement of biological molecules in the formation of nanoparticles (NPs). SEM revealed that the NPs were of approximately 40 nm. Overall, this study suggested that the biogenically synthesized nanoparticles could be utilized as effective antimicrobial agents for effective disease control.

Authors+Show Affiliations

Microbiology Lab, Department of Zoology, GC University.Microbiology Lab, Department of Zoology, GC University.Department of Zoology, University of Azad Jammu and Kashmir.Department of Zoology, University of Education, Lower Mall Campus.Department of Microbiology, University of Karachi.Department of Chemistry, University of Lahore.Microbiology Lab, Department of Zoology, GC University.Microbiology Lab, Department of Zoology, GC University.Department of Zoology, The Women University.Department of Zoology, The University of Lahore.Department of Pathology, University of Veterinary and Animal Sciences.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35034942

Citation

Tufail, Mohammad Shahzad, et al. "Biogenic Synthesis, Characterization and Antibacterial Properties of Silver Nanoparticles Against Human Pathogens." Journal of Oleo Science, vol. 71, no. 2, 2022, pp. 257-265.
Tufail MS, Liaqat I, Andleeb S, et al. Biogenic Synthesis, Characterization and Antibacterial Properties of Silver Nanoparticles against Human Pathogens. J Oleo Sci. 2022;71(2):257-265.
Tufail, M. S., Liaqat, I., Andleeb, S., Naseem, S., Zafar, U., Sadiqa, A., Liaqat, I., Ali, N. M., Bibi, A., Arshad, N., & Saleem, G. (2022). Biogenic Synthesis, Characterization and Antibacterial Properties of Silver Nanoparticles against Human Pathogens. Journal of Oleo Science, 71(2), 257-265. https://doi.org/10.5650/jos.ess21291
Tufail MS, et al. Biogenic Synthesis, Characterization and Antibacterial Properties of Silver Nanoparticles Against Human Pathogens. J Oleo Sci. 2022 Feb 3;71(2):257-265. PubMed PMID: 35034942.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biogenic Synthesis, Characterization and Antibacterial Properties of Silver Nanoparticles against Human Pathogens. AU - Tufail,Mohammad Shahzad, AU - Liaqat,Iram, AU - Andleeb,Saiqa, AU - Naseem,Sajida, AU - Zafar,Urooj, AU - Sadiqa,Ayesha, AU - Liaqat,Irfana, AU - Ali,Nazish Mazhar, AU - Bibi,Asia, AU - Arshad,Najma, AU - Saleem,Gulbeena, Y1 - 2022/01/14/ PY - 2022/1/18/pubmed PY - 2022/2/15/medline PY - 2022/1/17/entrez KW - biogenic silver nanoparticles KW - MIC and MBC determination KW - agar well diffusion method KW - antibacterial activity KW - tube dilution method SP - 257 EP - 265 JF - Journal of oleo science JO - J Oleo Sci VL - 71 IS - 2 N2 - Biogenic synthesis of silver nanoparticles (AgNPs) is more eco-friendly and cost-effective approach as compared to the conventional chemical synthesis. Biologically synthesized AgNPs have been proved as therapeutically effective and valuable compounds. In this study, the four bacterial strains Escherichia coli (MT448673), Pseudomonas aeruginosa (MN900691), Bacillus subtilis (MN900684) and Bacillus licheniformis (MN900686) were used for the biogenic synthesis of AgNPs. Agar well diffusion assay revealed to determine the antibacterial activity of all biogenically synthesized AGNPs showed that P. aeruginosa AgNPs possessed significantly high (p < 0.05) antibacterial potential against all tested isolates. The one-way ANOVA test showed that that P. aeruginosa AgNPs showed significantly (p < 0.05) larger zones of inhibition (ZOI: 19 to 22 mm) compared to the positive control (rifampicin: 50 µg/mL) while no ZOI was observed against negative control (Dimethyl sulfoxide: DMSO). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) concentration against four test strains also showed that among all biogenically synthesized NPs, P. aeruginosa AgNPs showed effective MIC (3.3-3.6 µg/mL) and MBC (4.3-4.6 µg/mL). Hence, P. aeruginosa AGNPs were characterized using visual UV vis-spectroscopy, X-ray diffractometer (XRD), fourier transform infrared (FTIR) and scanning electron microscopy (SEM). The formation of peak around 430 nm indicated the formation of AgNPs while the FTIR confirmed the involvement of biological molecules in the formation of nanoparticles (NPs). SEM revealed that the NPs were of approximately 40 nm. Overall, this study suggested that the biogenically synthesized nanoparticles could be utilized as effective antimicrobial agents for effective disease control. SN - 1347-3352 UR - https://www.unboundmedicine.com/medline/citation/35034942/Biogenic_Synthesis_Characterization_and_Antibacterial_Properties_of_Silver_Nanoparticles_against_Human_Pathogens_ L2 - https://dx.doi.org/10.5650/jos.ess21291 DB - PRIME DP - Unbound Medicine ER -