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Fungal endophytes vary by species, tissue type, and life cycle stage in intertidal macroalgae.
J Phycol. 2022 04; 58(2):330-342.JP

Abstract

Fungal symbionts of terrestrial plants are among the most widespread and well-studied symbioses, relatively little is known about fungi that are associated with macroalgae. To fill the gap in marine fungal taxonomy, we combined simple culture methods with amplicon sequencing to characterize the fungal communities associated with three brown (Sargassum muticum, Pelvetia canaliculata, and Himanthalia elongata) and two red (Mastocarpus stellatus and Chondrus crispus) macroalgae from one intertidal zone. In addition to characterizing novel fungal diversity, we tested three hypotheses: fungal diversity and community composition vary (i) among species distributed at different tidal heights, (ii) among tissue types (apices, mid-thallus, and stipe), and (iii) among "isomorphic" C. crispus life cycle stages. Almost 70% of our reads were classified as Ascomycota, 29% as Basidiomycota, and 1% that could not be classified to a phylum. Thirty fungal isolates were obtained, 18 of which were also detected with amplicon sequencing. Fungal communities differed by host and tissue type. Interestingly, P. canaliculata, a fucoid at the extreme high intertidal, did not show differences in fungal diversity across the thallus. As found in filamentous algal endophytes, fungal diversity varied among the three life cycle stages in C. crispus. Female gametophytes were also compositionally more dispersed as compared to the fewer variable tetrasporophytes and male gametophytes. We demonstrate the utility of combining relatively simple cultivation and sequencing approaches to characterize and study macroalgal-fungal associations and highlight the need to understand the role of fungi in near-shore marine ecosystems.

Authors+Show Affiliations

Institute for Chemistry and Biology of the Marine environment (ICBM), Carl-von-Ossietzky University Oldenburg, Schleusenstrasse 1, Wilhelmshaven, 26382, Germany. GEOMAR Helmholtz Centre for Ocean Research Kiel, Düsternbrooker Weg 20, Kiel, 24105, Germany.Marine Biological Association, The Laboratory, Citadel Hill, Plymouth, UK. School of Biological and Marine Sciences, University of Plymouth, Plymouth, UK.Marine Biological Association, The Laboratory, Citadel Hill, Plymouth, UK.Marine Biological Association, The Laboratory, Citadel Hill, Plymouth, UK. School of Biological and Marine Sciences, University of Plymouth, Plymouth, UK.Department of Biology, University of Alabama at Birmingham, 1300 University Blvd, Birmingham, Alabama, 35294, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

35090190

Citation

Bonthond, Guido, et al. "Fungal Endophytes Vary By Species, Tissue Type, and Life Cycle Stage in Intertidal Macroalgae." Journal of Phycology, vol. 58, no. 2, 2022, pp. 330-342.
Bonthond G, Barilo A, Allen RJ, et al. Fungal endophytes vary by species, tissue type, and life cycle stage in intertidal macroalgae. J Phycol. 2022;58(2):330-342.
Bonthond, G., Barilo, A., Allen, R. J., Cunliffe, M., & Krueger-Hadfield, S. A. (2022). Fungal endophytes vary by species, tissue type, and life cycle stage in intertidal macroalgae. Journal of Phycology, 58(2), 330-342. https://doi.org/10.1111/jpy.13237
Bonthond G, et al. Fungal Endophytes Vary By Species, Tissue Type, and Life Cycle Stage in Intertidal Macroalgae. J Phycol. 2022;58(2):330-342. PubMed PMID: 35090190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fungal endophytes vary by species, tissue type, and life cycle stage in intertidal macroalgae. AU - Bonthond,Guido, AU - Barilo,Anastasiia, AU - Allen,Ro J, AU - Cunliffe,Michael, AU - Krueger-Hadfield,Stacy A, Y1 - 2022/03/04/ PY - 2022/01/03/revised PY - 2021/06/14/received PY - 2022/01/04/accepted PY - 2022/1/29/pubmed PY - 2022/4/15/medline PY - 2022/1/28/entrez KW - evolutionary ecology KW - fungi KW - holobiont KW - life cycle KW - marine KW - mycology KW - seaweed KW - symbiosis SP - 330 EP - 342 JF - Journal of phycology JO - J Phycol VL - 58 IS - 2 N2 - Fungal symbionts of terrestrial plants are among the most widespread and well-studied symbioses, relatively little is known about fungi that are associated with macroalgae. To fill the gap in marine fungal taxonomy, we combined simple culture methods with amplicon sequencing to characterize the fungal communities associated with three brown (Sargassum muticum, Pelvetia canaliculata, and Himanthalia elongata) and two red (Mastocarpus stellatus and Chondrus crispus) macroalgae from one intertidal zone. In addition to characterizing novel fungal diversity, we tested three hypotheses: fungal diversity and community composition vary (i) among species distributed at different tidal heights, (ii) among tissue types (apices, mid-thallus, and stipe), and (iii) among "isomorphic" C. crispus life cycle stages. Almost 70% of our reads were classified as Ascomycota, 29% as Basidiomycota, and 1% that could not be classified to a phylum. Thirty fungal isolates were obtained, 18 of which were also detected with amplicon sequencing. Fungal communities differed by host and tissue type. Interestingly, P. canaliculata, a fucoid at the extreme high intertidal, did not show differences in fungal diversity across the thallus. As found in filamentous algal endophytes, fungal diversity varied among the three life cycle stages in C. crispus. Female gametophytes were also compositionally more dispersed as compared to the fewer variable tetrasporophytes and male gametophytes. We demonstrate the utility of combining relatively simple cultivation and sequencing approaches to characterize and study macroalgal-fungal associations and highlight the need to understand the role of fungi in near-shore marine ecosystems. SN - 1529-8817 UR - https://www.unboundmedicine.com/medline/citation/35090190/Fungal_endophytes_vary_by_species_tissue_type_and_life_cycle_stage_in_intertidal_macroalgae_ L2 - https://doi.org/10.1111/jpy.13237 DB - PRIME DP - Unbound Medicine ER -