Tags

Type your tag names separated by a space and hit enter

Vitamin D metabolites do not alter parathyroid hormone secretion acutely.
Bone Miner. 1986 Dec; 1(6):495-505.BM

Abstract

Evidence to date has failed to show a consistent effect of vitamin D metabolites on PTH secretion. This study was undertaken to assess the possible direct, acute effects of vitamin D metabolites on PTH secretion in vitro. Ethanol has been used in several published studies as the vehicle for vitamin D metabolites. We found that 0.2-1.0% ethanol inhibited PTH release from dispersed bovine parathyroid cells (PTC). Our experiments with vitamin D metabolites used ethanol as a vehicle at a concentration less than 0.1%. When compared to ethanol treatment, 10-100 nM 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), 25 and 100 nM 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) and 100 nM 1,24,25-trihydroxyvitamin D3 (1,24,25(OH)3D3) had no effect on PTH release from PTC incubated for up to 4 h. A combination of 1,25(OH)2D3 and 24,25(OH)2D3 (each 25 or 100 nM) was without effect. Also, 100 nM 1,25(OH)2D3 had no effect on PTH release from either bovine parathyroid gland slices or from parathyroid glands from either vitamin D-replete (+D) or vitamin D-deficient (-D) rats incubated for up to 4 h. The i.v. injection of 1 microgram 1.25(OH)2D3 into -D rats had no effect on either serum PTH or calcium (Ca), either 0.5 or 1.0 h after treatment. Parathyroid glands from -D rats incubated with 0.75 mM Ca secreted more PTH than glands of similar weight from rats given 25 micrograms vitamin D3 3 days earlier, suggesting that vitamin D or a metabolite of vitamin D may modulate the sensitivity of the parathyroid gland to medium Ca. In summary, we found no evidence for a direct, acute effect of vitamin D metabolites on PTH secretion under diverse experimental conditions.

Authors+Show Affiliations

Department of Pharmacology, University of Missouri School of Medicine, Columbia 65211.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

3509783

Citation

Magliola, L, et al. "Vitamin D Metabolites Do Not Alter Parathyroid Hormone Secretion Acutely." Bone and Mineral, vol. 1, no. 6, 1986, pp. 495-505.
Magliola L, Anast CS, Forte LR. Vitamin D metabolites do not alter parathyroid hormone secretion acutely. Bone Miner. 1986;1(6):495-505.
Magliola, L., Anast, C. S., & Forte, L. R. (1986). Vitamin D metabolites do not alter parathyroid hormone secretion acutely. Bone and Mineral, 1(6), 495-505.
Magliola L, Anast CS, Forte LR. Vitamin D Metabolites Do Not Alter Parathyroid Hormone Secretion Acutely. Bone Miner. 1986;1(6):495-505. PubMed PMID: 3509783.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vitamin D metabolites do not alter parathyroid hormone secretion acutely. AU - Magliola,L, AU - Anast,C S, AU - Forte,L R, PY - 1986/12/1/pubmed PY - 1986/12/1/medline PY - 1986/12/1/entrez SP - 495 EP - 505 JF - Bone and mineral JO - Bone Miner VL - 1 IS - 6 N2 - Evidence to date has failed to show a consistent effect of vitamin D metabolites on PTH secretion. This study was undertaken to assess the possible direct, acute effects of vitamin D metabolites on PTH secretion in vitro. Ethanol has been used in several published studies as the vehicle for vitamin D metabolites. We found that 0.2-1.0% ethanol inhibited PTH release from dispersed bovine parathyroid cells (PTC). Our experiments with vitamin D metabolites used ethanol as a vehicle at a concentration less than 0.1%. When compared to ethanol treatment, 10-100 nM 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), 25 and 100 nM 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) and 100 nM 1,24,25-trihydroxyvitamin D3 (1,24,25(OH)3D3) had no effect on PTH release from PTC incubated for up to 4 h. A combination of 1,25(OH)2D3 and 24,25(OH)2D3 (each 25 or 100 nM) was without effect. Also, 100 nM 1,25(OH)2D3 had no effect on PTH release from either bovine parathyroid gland slices or from parathyroid glands from either vitamin D-replete (+D) or vitamin D-deficient (-D) rats incubated for up to 4 h. The i.v. injection of 1 microgram 1.25(OH)2D3 into -D rats had no effect on either serum PTH or calcium (Ca), either 0.5 or 1.0 h after treatment. Parathyroid glands from -D rats incubated with 0.75 mM Ca secreted more PTH than glands of similar weight from rats given 25 micrograms vitamin D3 3 days earlier, suggesting that vitamin D or a metabolite of vitamin D may modulate the sensitivity of the parathyroid gland to medium Ca. In summary, we found no evidence for a direct, acute effect of vitamin D metabolites on PTH secretion under diverse experimental conditions. SN - 0169-6009 UR - https://www.unboundmedicine.com/medline/citation/3509783/Vitamin_D_metabolites_do_not_alter_parathyroid_hormone_secretion_acutely_ L2 - https://medlineplus.gov/vitamind.html DB - PRIME DP - Unbound Medicine ER -