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Oligo-FISH Paints in Triticeae.
Curr Protoc. 2022 Feb; 2(2):e364.CP

Abstract

We developed seven oligonucleotide (oligo) pools based on single-copy sequences, targeting chromosomes 1 to 7 of barley (Hordeum vulgare L.) and wheat (Triticum aestivum) for chromosomal Oligo-FISH painting methods. The probes were applied to high-throughput karyotyping for the Triticeae tribe of over 350 species including 30 genera such as Triticum, Hordeum, Secale, Aegilops, Thinopyrum, and Dasypyrum, as well as several wheat alien-derived lines. In combination with other nondenaturing FISH (ND-FISH) procedures using tandem-repeat oligos, the newly developed Oligo-FISH painting technique provides an efficient tool for the identification of individual chromosomes with homologous linkage groups to establish standard karyotypes, particularly with any wild Triticeae species having nonsequenced genomes for chromosome evolutionary analysis. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Oligo-pool probe development Basic Protocol 2: Nondenaturing FISH Basic Protocol 3: Oligo-FISH painting.

Authors+Show Affiliations

Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35157369

Citation

Li, Guangrong, and Zujun Yang. "Oligo-FISH Paints in Triticeae." Current Protocols, vol. 2, no. 2, 2022, pp. e364.
Li G, Yang Z. Oligo-FISH Paints in Triticeae. Curr Protoc. 2022;2(2):e364.
Li, G., & Yang, Z. (2022). Oligo-FISH Paints in Triticeae. Current Protocols, 2(2), e364. https://doi.org/10.1002/cpz1.364
Li G, Yang Z. Oligo-FISH Paints in Triticeae. Curr Protoc. 2022;2(2):e364. PubMed PMID: 35157369.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oligo-FISH Paints in Triticeae. AU - Li,Guangrong, AU - Yang,Zujun, PY - 2022/2/14/entrez PY - 2022/2/15/pubmed PY - 2022/2/17/medline KW - Oligo-FISH KW - Triticeae KW - karyotyping SP - e364 EP - e364 JF - Current protocols JO - Curr Protoc VL - 2 IS - 2 N2 - We developed seven oligonucleotide (oligo) pools based on single-copy sequences, targeting chromosomes 1 to 7 of barley (Hordeum vulgare L.) and wheat (Triticum aestivum) for chromosomal Oligo-FISH painting methods. The probes were applied to high-throughput karyotyping for the Triticeae tribe of over 350 species including 30 genera such as Triticum, Hordeum, Secale, Aegilops, Thinopyrum, and Dasypyrum, as well as several wheat alien-derived lines. In combination with other nondenaturing FISH (ND-FISH) procedures using tandem-repeat oligos, the newly developed Oligo-FISH painting technique provides an efficient tool for the identification of individual chromosomes with homologous linkage groups to establish standard karyotypes, particularly with any wild Triticeae species having nonsequenced genomes for chromosome evolutionary analysis. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Oligo-pool probe development Basic Protocol 2: Nondenaturing FISH Basic Protocol 3: Oligo-FISH painting. SN - 2691-1299 UR - https://www.unboundmedicine.com/medline/citation/35157369/Oligo_FISH_Paints_in_Triticeae_ L2 - https://doi.org/10.1002/cpz1.364 DB - PRIME DP - Unbound Medicine ER -
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