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The colonization of Peyer's patches by a strain of Salmonella typhimurium cured of the cryptic plasmid.
J Infect Dis. 1986 Jun; 153(6):1119-25.JI

Abstract

We cured a strain of Salmonella typhimurium of its cryptic plasmid and confirmed that orally administered cured strains lost virulence for mice. Loss of the cryptic plasmid rendered the S. typhimurium strain sensitive to the bactericidal action of normal human serum. However, loss of the plasmid did not change the ability of the strain to associate with HeLa cells in tissue culture. Furthermore, when administered orally to mice, both the plasmid-containing and plasmid-free strains invaded the Peyer's patches of the small intestine to the same extent, and both were capable of inducing resistance to oral challenge with virulent S. typhimurium. When injected intraperitoneally, the cured strain was eliminated rapidly, whereas the parental strain persisted. We also showed that the cured strain did not contain a plasmid copy in the chromosome. We propose that although the plasmid-cured strain of S. typhimurium is able to colonize Peyer's patches, it cannot survive when administered intraperitoneally because it is susceptible to elimination by macrophages.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3517188

Citation

Hackett, J, et al. "The Colonization of Peyer's Patches By a Strain of Salmonella Typhimurium Cured of the Cryptic Plasmid." The Journal of Infectious Diseases, vol. 153, no. 6, 1986, pp. 1119-25.
Hackett J, Kotlarski I, Mathan V, et al. The colonization of Peyer's patches by a strain of Salmonella typhimurium cured of the cryptic plasmid. J Infect Dis. 1986;153(6):1119-25.
Hackett, J., Kotlarski, I., Mathan, V., Francki, K., & Rowley, D. (1986). The colonization of Peyer's patches by a strain of Salmonella typhimurium cured of the cryptic plasmid. The Journal of Infectious Diseases, 153(6), 1119-25.
Hackett J, et al. The Colonization of Peyer's Patches By a Strain of Salmonella Typhimurium Cured of the Cryptic Plasmid. J Infect Dis. 1986;153(6):1119-25. PubMed PMID: 3517188.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The colonization of Peyer's patches by a strain of Salmonella typhimurium cured of the cryptic plasmid. AU - Hackett,J, AU - Kotlarski,I, AU - Mathan,V, AU - Francki,K, AU - Rowley,D, PY - 1986/6/1/pubmed PY - 1986/6/1/medline PY - 1986/6/1/entrez SP - 1119 EP - 25 JF - The Journal of infectious diseases JO - J Infect Dis VL - 153 IS - 6 N2 - We cured a strain of Salmonella typhimurium of its cryptic plasmid and confirmed that orally administered cured strains lost virulence for mice. Loss of the cryptic plasmid rendered the S. typhimurium strain sensitive to the bactericidal action of normal human serum. However, loss of the plasmid did not change the ability of the strain to associate with HeLa cells in tissue culture. Furthermore, when administered orally to mice, both the plasmid-containing and plasmid-free strains invaded the Peyer's patches of the small intestine to the same extent, and both were capable of inducing resistance to oral challenge with virulent S. typhimurium. When injected intraperitoneally, the cured strain was eliminated rapidly, whereas the parental strain persisted. We also showed that the cured strain did not contain a plasmid copy in the chromosome. We propose that although the plasmid-cured strain of S. typhimurium is able to colonize Peyer's patches, it cannot survive when administered intraperitoneally because it is susceptible to elimination by macrophages. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/3517188/The_colonization_of_Peyer's_patches_by_a_strain_of_Salmonella_typhimurium_cured_of_the_cryptic_plasmid_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/153.6.1119 DB - PRIME DP - Unbound Medicine ER -