Tags

Type your tag names separated by a space and hit enter

Exogenous sucrose influences KEA1 and KEA2 to regulate abscisic acid-mediated primary root growth in Arabidopsis.
Plant Sci. 2022 Apr; 317:111209.PS

Abstract

Arabidopsis K+-efflux antiporter (KEA)1 and KEA2 are chloroplast inner envelope membrane K+/H+ antiporters that play an important role in plastid development and seedling growth. However, the function of KEA1 and KEA2 during early seedling development is poorly understood. In this work, we found that in Arabidopsis, KEA1 and KEA2 mediated primary root growth by regulating photosynthesis and the ABA signaling pathway. Phenotypic analyses revealed that in the absence of sucrose, the primary root length of the kea1kea2 mutant was significantly shorter than that of the wild-type Columbia-0 (Col-0) plant. However, this phenotype could be remedied by the external application of sucrose. Meanwhile, HPLC-MS/MS results showed that in sucrose-free medium, ABA accumulation in the kea1kea2 mutant was considerably lower than that in Col-0. Transcriptome analysis revealed that many key genes involved in ABA signals were repressed in the kea1kea2 mutant. We concluded that KEA1 and KEA2 deficiency not only affected photosynthesis but was also involved in primary root growth likely through an ABA-dependent manner. This study confirmed the new function of KEA1 and KEA2 in affecting primary root growth.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

College of Life Sciences, Northwest Normal University, Lanzhou 730070, China; Academy of Plateau Science and Sustainability, Qinghai Normal University, Xining 810016, China. Electronic address: zhengsheng@nwnu.edu.cn.

College of Life Sciences, Northwest Normal University, Lanzhou 730070, China.

College of Life Sciences, Northwest Normal University, Lanzhou 730070, China.

College of Life Sciences, Northwest Normal University, Lanzhou 730070, China.

College of Life Sciences, Northwest Normal University, Lanzhou 730070, China.

College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.

College of Life Sciences, Northwest Normal University, Lanzhou 730070, China.

College of Life Sciences, Northwest Normal University, Lanzhou 730070, China.

College of Life Sciences, Northwest Normal University, Lanzhou 730070, China.

College of Life Sciences, Northwest Normal University, Lanzhou 730070, China.

College of Life Sciences, Northwest Normal University, Lanzhou 730070, China.

College of Life Sciences, Northwest Normal University, Lanzhou 730070, China. Electronic address: zhangtengguo@163.com.

MeSH

Abscisic AcidArabidopsisArabidopsis ProteinsGene Expression Regulation, PlantMutationPlant RootsPotassium-Hydrogen AntiportersSucroseTandem Mass Spectrometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35193734

Citation

Zheng, Sheng, et al. "Exogenous Sucrose Influences KEA1 and KEA2 to Regulate Abscisic Acid-mediated Primary Root Growth in Arabidopsis." Plant Science : an International Journal of Experimental Plant Biology, vol. 317, 2022, p. 111209.

Zheng S, Su M, Shi Z, et al. Exogenous sucrose influences KEA1 and KEA2 to regulate abscisic acid-mediated primary root growth in Arabidopsis. Plant Sci. 2022;317:111209.

Zheng, S., Su, M., Shi, Z., Gao, H., Ma, C., Zhu, S., Zhang, L., Wu, G., Wu, W., Wang, J., Zhang, J., & Zhang, T. (2022). Exogenous sucrose influences KEA1 and KEA2 to regulate abscisic acid-mediated primary root growth in Arabidopsis. Plant Science : an International Journal of Experimental Plant Biology, 317, 111209. https://doi.org/10.1016/j.plantsci.2022.111209

Zheng S, et al. Exogenous Sucrose Influences KEA1 and KEA2 to Regulate Abscisic Acid-mediated Primary Root Growth in Arabidopsis. Plant Sci. 2022;317:111209. PubMed PMID: 35193734.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Exogenous sucrose influences KEA1 and KEA2 to regulate abscisic acid-mediated primary root growth in Arabidopsis. AU - Zheng,Sheng, AU - Su,Min, AU - Shi,Zhongfei, AU - Gao,Haixia, AU - Ma,Cheng, AU - Zhu,Shan, AU - Zhang,Lina, AU - Wu,Guofan, AU - Wu,Wangze, AU - Wang,Juan, AU - Zhang,Jinping, AU - Zhang,Tengguo, Y1 - 2022/02/04/ PY - 2021/11/04/received PY - 2022/01/24/revised PY - 2022/02/03/accepted PY - 2022/2/23/entrez PY - 2022/2/24/pubmed PY - 2022/2/25/medline KW - Abscisic acid KW - Arabidopsis KW - K(+)-efflux antiporters KW - Root growth KW - Sucrose SP - 111209 EP - 111209 JF - Plant science : an international journal of experimental plant biology JO - Plant Sci VL - 317 N2 - Arabidopsis K+-efflux antiporter (KEA)1 and KEA2 are chloroplast inner envelope membrane K+/H+ antiporters that play an important role in plastid development and seedling growth. However, the function of KEA1 and KEA2 during early seedling development is poorly understood. In this work, we found that in Arabidopsis, KEA1 and KEA2 mediated primary root growth by regulating photosynthesis and the ABA signaling pathway. Phenotypic analyses revealed that in the absence of sucrose, the primary root length of the kea1kea2 mutant was significantly shorter than that of the wild-type Columbia-0 (Col-0) plant. However, this phenotype could be remedied by the external application of sucrose. Meanwhile, HPLC-MS/MS results showed that in sucrose-free medium, ABA accumulation in the kea1kea2 mutant was considerably lower than that in Col-0. Transcriptome analysis revealed that many key genes involved in ABA signals were repressed in the kea1kea2 mutant. We concluded that KEA1 and KEA2 deficiency not only affected photosynthesis but was also involved in primary root growth likely through an ABA-dependent manner. This study confirmed the new function of KEA1 and KEA2 in affecting primary root growth. SN - 1873-2259 UR - https://www.unboundmedicine.com/medline/citation/35193734/Exogenous_sucrose_influences_KEA1_and_KEA2_to_regulate_abscisic_acid_mediated_primary_root_growth_in_Arabidopsis_ DB - PRIME DP - Unbound Medicine ER -