TY - JOUR T1 - Identification and DNA Marker Development for a Wheat-Leymus mollis 2Ns (2D) Disomic Chromosome Substitution. AU - Feng,Xianbo, AU - Du,Xin, AU - Wang,Siwen, AU - Deng,Pingchuan, AU - Wang,Yongfu, AU - Shang,Lihui, AU - Tian,Zengrong, AU - Wang,Changyou, AU - Chen,Chunhuan, AU - Zhao,Jixin, AU - Ji,Wanquan, Y1 - 2022/02/28/ PY - 2021/12/24/received PY - 2022/02/17/revised PY - 2022/02/27/accepted PY - 2022/3/10/entrez PY - 2022/3/11/pubmed PY - 2022/4/9/medline KW - Leymus mollis KW - SLAF-seq KW - molecular cytogenetics KW - stripe rust KW - wheat 55K array JF - International journal of molecular sciences JO - Int J Mol Sci VL - 23 IS - 5 N2 - Leymus mollis (2n = 4x = 28, NsNsXmXm), a wild relative of common wheat (Triticum aestivum L.), carries numerous loci which could potentially be used in wheat improvement. In this study, line 17DM48 was isolated from the progeny of a wheat and L. mollis hybrid. This line has 42 chromosomes forming 21 bivalents at meiotic metaphase I. Genomic in situ hybridization (GISH) demonstrated the presence of a pair chromosomes from the Ns genome of L. mollis. This pair substituted for wheat chromosome 2D, as shown by fluorescence in situ hybridization (FISH), DNA marker analysis, and hybridization to wheat 55K SNP array. Therefore, 17DM48 is a wheat-L. mollis 2Ns (2D) disomic substitution line. It shows longer spike and a high level of stripe rust resistance. Using specific-locus amplified fragment sequencing (SLAF-seq), 13 DNA markers were developed to identify and trace chromosome 2Ns of L. mollis in wheat background. This line provides a potential bridge germplasm for genetic improvement of wheat stripe rust resistance. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/35269816/Identification_and_DNA_Marker_Development_for_a_Wheat_Leymus_mollis_2Ns__2D__Disomic_Chromosome_Substitution_ DB - PRIME DP - Unbound Medicine ER -