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miRNA profiling of human nasopharyngeal carcinoma cell lines HONE1 and CNE2 after X-ray therapy.
Adv Clin Exp Med. 2022 Jun; 31(6):671-687.AC

Abstract

BACKGROUND

Radiotherapy is the main treatment for nasopharyngeal carcinoma. The radioresistance mechanism of cells is related to miRNAs.

OBJECTIVES

To investigate the miRNA profiling of HONE1 and CNE2 after X-ray therapy.

MATERIAL AND METHODS

The HONE1 and CNE2 cells were treated with X-ray at 4 Gy, 8 Gy, 16 Gy, and 20 Gy doses. The cell lines CNE2 with the best therapy effects and HONE1 with the worst therapy effects were screened out. Apoptosis and cell viability were detected with flow cytometry and Cell Counting Kit-8 (CCK-8). High-throughput sequencing was performed. A miRNA library was constructed. The miRNA annotation expression distribution, family prediction and target gene interaction, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were conducted.

RESULTS

The 24-hour 20 Gy dose X-rays were selected as the optimal therapy conditions. The CNE2_C, CNE2_M, HONE1_C and HONE1_M miRNAs accounted for 26.5%, 31.7%, 21.3%, and 22.9% of the Cleandata reads count, respectively, and the contents of rRNAs accounted for 24.9%, 14.7%, 25.1%, and 25.1% of the Cleandata reads count, respectively. The miRNAs with differential expression between the HONE1 and CNE2 cell lines including hsa-miR-21-5p, hsa-let-7a-5p, hsa-miR-125a-5p, hsa-miR-26a-5p, hsa-let-7f-5p, hsa-miR-20a-5p, and hsa-miR-24a-3p. There were also differentially expressed miRNAs in HONE1_C vs. HONE1_M, such as hsa-miR-21-5p and hsa-let-7i-5p. The differentially expressed miRNA in CNE2_C vs. CNE2_M was hsa-miR-148b-3p. The Gene Ontology analysis showed that the differentially expressed miRNA interacting genes in HONE1_M vs. CNE2_M were mainly enriched in biological process such as negative and positive regulation of transcription from RNA polymerase II promoter, cellular component such as cytosol and molecular function such as protein binding factor. The KEGG pathway analysis revealed that the differentially expressed miRNA interacting genes in HONE1_M vs. CNE2_M were enriched in the cancer-related pathways, such as pathways in cancer, MAPK signaling pathway and Wnt signaling pathway.

CONCLUSIONS

Twelve miRNAs and 9 genes which contribute to X-ray radiation resistance were identified. Among those with differential expression between the HONE1 and CNE2 cell lines, which played a regulatory role in multiple pathways, were hsa-miR-20a-5p, hsa-let-7a-5p, hsa-let-7f5p, hsa-let-7i-5p, hsa-miR-30e-5p, hsa-miR-148b-3p, and hsa-miR-200c-3p. The corresponding genes were MAPK1, SOS1, TGFBR1, TGFBR2, TP53, CASP3, CCNE2, PTEN, and CDK2.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Luo H
Department of Oncology, The First Affiliated Hospital of Nanchang University, China.
Zhong F
Department of Oncology, The First Affiliated Hospital of Nanchang University, China.
Jing X
Department of Oncology, Jiangxi Lushan People's Hospital, Jiujiang, China.
Lin H
Department of Oncology, The First Affiliated Hospital of Nanchang University, China.
Li Y
Department of Oncology, The First Affiliated Hospital of Nanchang University, China.

MeSH

Cell Line, TumorGene Expression ProfilingHumansMicroRNAsNasopharyngeal CarcinomaNasopharyngeal Neoplasms

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35275451

Citation

Luo, Hui, et al. "MiRNA Profiling of Human Nasopharyngeal Carcinoma Cell Lines HONE1 and CNE2 After X-ray Therapy." Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University, vol. 31, no. 6, 2022, pp. 671-687.
Luo H, Zhong F, Jing X, et al. MiRNA profiling of human nasopharyngeal carcinoma cell lines HONE1 and CNE2 after X-ray therapy. Adv Clin Exp Med. 2022;31(6):671-687.
Luo, H., Zhong, F., Jing, X., Lin, H., & Li, Y. (2022). MiRNA profiling of human nasopharyngeal carcinoma cell lines HONE1 and CNE2 after X-ray therapy. Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University, 31(6), 671-687. https://doi.org/10.17219/acem/146580
Luo H, et al. MiRNA Profiling of Human Nasopharyngeal Carcinoma Cell Lines HONE1 and CNE2 After X-ray Therapy. Adv Clin Exp Med. 2022;31(6):671-687. PubMed PMID: 35275451.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - miRNA profiling of human nasopharyngeal carcinoma cell lines HONE1 and CNE2 after X-ray therapy. AU - Luo,Hui, AU - Zhong,Fangyan, AU - Jing,Xiang, AU - Lin,Hong, AU - Li,Yong, PY - 2022/3/12/pubmed PY - 2022/6/30/medline PY - 2022/3/11/entrez KW - CNE2 KW - HONE1 KW - X-ray radiation resistance KW - human nasopharyngeal carcinoma KW - miRNA profiling SP - 671 EP - 687 JF - Advances in clinical and experimental medicine : official organ Wroclaw Medical University JO - Adv Clin Exp Med VL - 31 IS - 6 N2 - BACKGROUND: Radiotherapy is the main treatment for nasopharyngeal carcinoma. The radioresistance mechanism of cells is related to miRNAs. OBJECTIVES: To investigate the miRNA profiling of HONE1 and CNE2 after X-ray therapy. MATERIAL AND METHODS: The HONE1 and CNE2 cells were treated with X-ray at 4 Gy, 8 Gy, 16 Gy, and 20 Gy doses. The cell lines CNE2 with the best therapy effects and HONE1 with the worst therapy effects were screened out. Apoptosis and cell viability were detected with flow cytometry and Cell Counting Kit-8 (CCK-8). High-throughput sequencing was performed. A miRNA library was constructed. The miRNA annotation expression distribution, family prediction and target gene interaction, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were conducted. RESULTS: The 24-hour 20 Gy dose X-rays were selected as the optimal therapy conditions. The CNE2_C, CNE2_M, HONE1_C and HONE1_M miRNAs accounted for 26.5%, 31.7%, 21.3%, and 22.9% of the Cleandata reads count, respectively, and the contents of rRNAs accounted for 24.9%, 14.7%, 25.1%, and 25.1% of the Cleandata reads count, respectively. The miRNAs with differential expression between the HONE1 and CNE2 cell lines including hsa-miR-21-5p, hsa-let-7a-5p, hsa-miR-125a-5p, hsa-miR-26a-5p, hsa-let-7f-5p, hsa-miR-20a-5p, and hsa-miR-24a-3p. There were also differentially expressed miRNAs in HONE1_C vs. HONE1_M, such as hsa-miR-21-5p and hsa-let-7i-5p. The differentially expressed miRNA in CNE2_C vs. CNE2_M was hsa-miR-148b-3p. The Gene Ontology analysis showed that the differentially expressed miRNA interacting genes in HONE1_M vs. CNE2_M were mainly enriched in biological process such as negative and positive regulation of transcription from RNA polymerase II promoter, cellular component such as cytosol and molecular function such as protein binding factor. The KEGG pathway analysis revealed that the differentially expressed miRNA interacting genes in HONE1_M vs. CNE2_M were enriched in the cancer-related pathways, such as pathways in cancer, MAPK signaling pathway and Wnt signaling pathway. CONCLUSIONS: Twelve miRNAs and 9 genes which contribute to X-ray radiation resistance were identified. Among those with differential expression between the HONE1 and CNE2 cell lines, which played a regulatory role in multiple pathways, were hsa-miR-20a-5p, hsa-let-7a-5p, hsa-let-7f5p, hsa-let-7i-5p, hsa-miR-30e-5p, hsa-miR-148b-3p, and hsa-miR-200c-3p. The corresponding genes were MAPK1, SOS1, TGFBR1, TGFBR2, TP53, CASP3, CCNE2, PTEN, and CDK2. SN - 1899-5276 UR - https://www.unboundmedicine.com/medline/citation/35275451/miRNA_profiling_of_human_nasopharyngeal_carcinoma_cell_lines_HONE1_and_CNE2_after_X_ray_therapy_ DB - PRIME DP - Unbound Medicine ER -