Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat-Thinopyrum intermedium and wheat-Thinopyrum ponticum substitution lines.
BMC Plant Biol. 2022 Mar 12; 22(1):111.BP

Abstract

BACKGROUND

Owing to their excellent resistance to abiotic and biotic stress, Thinopyrum intermedium (2n = 6x = 42, JJJsJsStSt) and Th. ponticum (2n = 10x = 70) are both widely utilized in wheat germplasm innovation programs. Disomic substitution lines (DSLs) carrying one pair of alien chromosomes are valuable bridge materials for transmission of novel genes, fluorescence in situ hybridization (FISH) karyotype construction and specific molecular marker development.

RESULTS

Six wheat-Thinopyrum DSLs derived from crosses between Abbondanza nullisomic lines (2n = 40) and two octoploid Trititrigia lines (2n = 8x = 56), were characterized by sequential FISH-genome in situ hybridization (GISH), multicolor GISH (mc-GISH), and an analysis of the wheat 15 K SNP array combined with molecular marker selection. ES-9 (DS2St (2A)) and ES-10 (DS3St (3D)) are wheat-Th. ponticum DSLs, while ES-23 (DS2St (2A)), ES-24 (DS3St (3D)), ES-25(DS2St (2B)), and ES-26 (DS2St (2D)) are wheat-Th. intermedium DSLs. ES-9, ES-23, ES-25 and ES-26 conferred high thousand-kernel weight and stripe rust resistance at adult stages, while ES-10 and ES-24 were highly resistant to stripe rust at all stages. Furthermore, cytological analysis showed that the alien chromosomes belonging to the same homoeologous group (2 or 3) derived from different donors carried the same FISH karyotype and could form a bivalent. Based on specific-locus amplified fragment sequencing (SLAF-seq), two 2St-chromosome-specific markers (PTH-005 and PTH-013) and two 3St-chromosome-specific markers (PTH-113 and PTH-135) were developed.

CONCLUSIONS

The six wheat-Thinopyrum DSLs conferring stripe rust resistance can be used as bridging parents for transmission of valuable resistance genes. The utility of PTH-113 and PTH-135 in a BC1F2 population showed that the newly developed markers could be useful tools for efficient identification of St chromosomes in a common wheat background.

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Authors+Show Affiliations

Wang S

College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Wang C

College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, 712100, Shaanxi, China. State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China.

Feng X

College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China.

State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China. College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Ji W

College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China. jiwanquan2008@126.com. Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, 712100, Shaanxi, China. jiwanquan2008@126.com. State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China. jiwanquan2008@126.com.

MeSH

Chromosomes, PlantCytogenetic AnalysisDisease ResistanceGenetic MarkersGenetic VariationGenotypePoaceaePucciniaTriticum

Pub Type(s)

Comparative Study

Journal Article

Language

eng

PubMed ID

35279089

Citation

Wang, Siwen, et al. "Molecular Cytogenetics and Development of St-chromosome-specific Molecular Markers of Novel Stripe Rust Resistant wheat-Thinopyrum Intermedium and wheat-Thinopyrum Ponticum Substitution Lines." BMC Plant Biology, vol. 22, no. 1, 2022, p. 111.

Wang S, Wang C, Feng X, et al. Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat-Thinopyrum intermedium and wheat-Thinopyrum ponticum substitution lines. BMC Plant Biol. 2022;22(1):111.

Wang, S., Wang, C., Feng, X., Zhao, J., Deng, P., Wang, Y., Zhang, H., Liu, X., Li, T., Chen, C., Wang, B., & Ji, W. (2022). Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat-Thinopyrum intermedium and wheat-Thinopyrum ponticum substitution lines. BMC Plant Biology, 22(1), 111. https://doi.org/10.1186/s12870-022-03496-x

Wang S, et al. Molecular Cytogenetics and Development of St-chromosome-specific Molecular Markers of Novel Stripe Rust Resistant wheat-Thinopyrum Intermedium and wheat-Thinopyrum Ponticum Substitution Lines. BMC Plant Biol. 2022 Mar 12;22(1):111. PubMed PMID: 35279089.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat-Thinopyrum intermedium and wheat-Thinopyrum ponticum substitution lines. AU - Wang,Siwen, AU - Wang,Changyou, AU - Feng,Xianbo, AU - Zhao,Jixin, AU - Deng,Pingchuan, AU - Wang,Yajuan, AU - Zhang,Hong, AU - Liu,Xinlun, AU - Li,Tingdong, AU - Chen,Chunhuan, AU - Wang,Baotong, AU - Ji,Wanquan, Y1 - 2022/03/12/ PY - 2021/09/16/received PY - 2022/02/24/accepted PY - 2022/3/13/entrez PY - 2022/3/14/pubmed PY - 2022/3/22/medline KW - St-chromosome-specific molecular markers KW - Stripe rust resistance KW - Substitution lines KW - Thinopyrum intermedium KW - Thinopyrum ponticum SP - 111 EP - 111 JF - BMC plant biology JO - BMC Plant Biol VL - 22 IS - 1 N2 - BACKGROUND: Owing to their excellent resistance to abiotic and biotic stress, Thinopyrum intermedium (2n = 6x = 42, JJJsJsStSt) and Th. ponticum (2n = 10x = 70) are both widely utilized in wheat germplasm innovation programs. Disomic substitution lines (DSLs) carrying one pair of alien chromosomes are valuable bridge materials for transmission of novel genes, fluorescence in situ hybridization (FISH) karyotype construction and specific molecular marker development. RESULTS: Six wheat-Thinopyrum DSLs derived from crosses between Abbondanza nullisomic lines (2n = 40) and two octoploid Trititrigia lines (2n = 8x = 56), were characterized by sequential FISH-genome in situ hybridization (GISH), multicolor GISH (mc-GISH), and an analysis of the wheat 15 K SNP array combined with molecular marker selection. ES-9 (DS2St (2A)) and ES-10 (DS3St (3D)) are wheat-Th. ponticum DSLs, while ES-23 (DS2St (2A)), ES-24 (DS3St (3D)), ES-25(DS2St (2B)), and ES-26 (DS2St (2D)) are wheat-Th. intermedium DSLs. ES-9, ES-23, ES-25 and ES-26 conferred high thousand-kernel weight and stripe rust resistance at adult stages, while ES-10 and ES-24 were highly resistant to stripe rust at all stages. Furthermore, cytological analysis showed that the alien chromosomes belonging to the same homoeologous group (2 or 3) derived from different donors carried the same FISH karyotype and could form a bivalent. Based on specific-locus amplified fragment sequencing (SLAF-seq), two 2St-chromosome-specific markers (PTH-005 and PTH-013) and two 3St-chromosome-specific markers (PTH-113 and PTH-135) were developed. CONCLUSIONS: The six wheat-Thinopyrum DSLs conferring stripe rust resistance can be used as bridging parents for transmission of valuable resistance genes. The utility of PTH-113 and PTH-135 in a BC1F2 population showed that the newly developed markers could be useful tools for efficient identification of St chromosomes in a common wheat background. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/35279089/Molecular_cytogenetics_and_development_of_St_chromosome_specific_molecular_markers_of_novel_stripe_rust_resistant_wheat_Thinopyrum_intermedium_and_wheat_Thinopyrum_ponticum_substitution_lines_ DB - PRIME DP - Unbound Medicine ER -

Tags

Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat-Thinopyrum intermedium and wheat-Thinopyrum ponticum substitution lines.BMC Plant Biol. 2022 Mar 12; 22(1):111.BP