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[Genetic and heteroduplex analysis of deletion mutants of plasmid pAS8].
Genetika. 1978 May; 14(5):853-66.G

Abstract

Heteroduplex analysis of deletion mutants of plasmid pAS8 permitted to construct a physical map and to elaborate in greater detail the genetic map of RP4 plasmid. The correlation between the ability of mutants to replicate in cells lacking functional DNA-polymerase I and the length of the deleted segment permitted to map rep genes of RP4 on DNA region with coordinates 9.8-17.3 kb. A relationship between the manifestation of incompatibility of mutants with IncP-1 plasmids and the length of deletions indicates that inc genes(s) are located on DNA region with coordinates 2.1-9.8 kb. The analysis of replication of deletion mutants and the manifestation of incompatibility just as of the data about the size of appropriate deletions permitted to make the conclusion about the functional and genetic non-identity of the replication control and incompatibility control in RP4 plasmid. Different degrees of incompatibility manifested by different plasmids suggest a possible polygenic control of the incompatibility.

Authors

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Pub Type(s)

Comparative Study
English Abstract
Journal Article

Language

rus

PubMed ID

352803

Citation

Sakanian, V A., et al. "[Genetic and Heteroduplex Analysis of Deletion Mutants of Plasmid PAS8]." Genetika, vol. 14, no. 5, 1978, pp. 853-66.
Sakanian VA, Iakubov IZ, Alikhanian SI, et al. [Genetic and heteroduplex analysis of deletion mutants of plasmid pAS8]. Genetika. 1978;14(5):853-66.
Sakanian, V. A., Iakubov, I. Z., Alikhanian, S. I., & Stepanov, A. I. (1978). [Genetic and heteroduplex analysis of deletion mutants of plasmid pAS8]. Genetika, 14(5), 853-66.
Sakanian VA, et al. [Genetic and Heteroduplex Analysis of Deletion Mutants of Plasmid PAS8]. Genetika. 1978;14(5):853-66. PubMed PMID: 352803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Genetic and heteroduplex analysis of deletion mutants of plasmid pAS8]. AU - Sakanian,V A, AU - Iakubov,I Z, AU - Alikhanian,S I, AU - Stepanov,A I, PY - 1978/5/1/pubmed PY - 1978/5/1/medline PY - 1978/5/1/entrez SP - 853 EP - 66 JF - Genetika JO - Genetika VL - 14 IS - 5 N2 - Heteroduplex analysis of deletion mutants of plasmid pAS8 permitted to construct a physical map and to elaborate in greater detail the genetic map of RP4 plasmid. The correlation between the ability of mutants to replicate in cells lacking functional DNA-polymerase I and the length of the deleted segment permitted to map rep genes of RP4 on DNA region with coordinates 9.8-17.3 kb. A relationship between the manifestation of incompatibility of mutants with IncP-1 plasmids and the length of deletions indicates that inc genes(s) are located on DNA region with coordinates 2.1-9.8 kb. The analysis of replication of deletion mutants and the manifestation of incompatibility just as of the data about the size of appropriate deletions permitted to make the conclusion about the functional and genetic non-identity of the replication control and incompatibility control in RP4 plasmid. Different degrees of incompatibility manifested by different plasmids suggest a possible polygenic control of the incompatibility. SN - 0016-6758 UR - https://www.unboundmedicine.com/medline/citation/352803/[Genetic_and_heteroduplex_analysis_of_deletion_mutants_of_plasmid_pAS8]_ DB - PRIME DP - Unbound Medicine ER -