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IgM and IgG antibodies to phenolic glycolipid I from Mycobacterium leprae in leprosy: insight into patient monitoring, erythema nodosum leprosum, and bacillary persistence.
J Invest Dermatol. 1986 May; 86(5):529-34.JI

Abstract

Serum IgM and IgG antibodies against Mycobacterium leprae-derived phenolic glycolipid I (PG) were determined in leprosy patients, contacts, and controls by enzyme-linked immunosorbent assay (ELISA). Anti-PG IgM levels increased from the tuberculoid (TT) to the lepromatous (LL) pole of the disease spectrum. There was a positive linear correlation between anti-PG IgM and bacillary index (BI). Patients with erythema nodosum leprosum (ENL) had lower levels of serum anti-PG IgM than non-ENL patients of comparable BI, suggesting that anti-PG IgM is involved in the pathogenesis of ENL. Initial observations indicate that high anti-PG IgM levels in bacillary-negative patients might reflect bacillary persistence. A study of 2 different substrate reagents in the ELISA [2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 0.1 mM H2O2, serum diluted 1:20, and o-phenylenediamine (OPD), 5 mM H2O2, serum diluted 1:300] showed generally good correlation in detection of anti-PG IgM. However the OPD system detected more paucibacillary disease (BT), while the ABTS system detected the significant effect of ENL on the relationship between BI and anti-PG IgM. Anti-PG IgM was clearly dominant over anti-PG IgG. However, certain patients, including several patients who had upgraded from LL and borderline lepromatous leprosy (BL), showed high levels of anti-PG IgG. Since studies have shown that LL patients are selectively deficient in cell-mediated immunity, T-cell products may be required for the IgM to IgG isotype switch. We conclude that anti-PG IgM is useful for monitoring the bacillary load in individual patients and should prove useful for leprosy control strategies.

Authors

Levis WR
No affiliation info available
Meeker HC
No affiliation info available
Schuller-Levis G
No affiliation info available
Sersen E
No affiliation info available
Schwerer B
No affiliation info available

MeSH

Antibodies, BacterialAntigens, BacterialBinding Sites, AntibodyEnzyme-Linked Immunosorbent AssayErythema NodosumGlycolipidsHumansImmunoglobulin GImmunoglobulin MLeprosyMycobacterium leprae

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3528312

Citation

Levis, W R., et al. "IgM and IgG Antibodies to Phenolic Glycolipid I From Mycobacterium Leprae in Leprosy: Insight Into Patient Monitoring, Erythema Nodosum Leprosum, and Bacillary Persistence." The Journal of Investigative Dermatology, vol. 86, no. 5, 1986, pp. 529-34.
Levis WR, Meeker HC, Schuller-Levis G, et al. IgM and IgG antibodies to phenolic glycolipid I from Mycobacterium leprae in leprosy: insight into patient monitoring, erythema nodosum leprosum, and bacillary persistence. J Invest Dermatol. 1986;86(5):529-34.
Levis, W. R., Meeker, H. C., Schuller-Levis, G., Sersen, E., & Schwerer, B. (1986). IgM and IgG antibodies to phenolic glycolipid I from Mycobacterium leprae in leprosy: insight into patient monitoring, erythema nodosum leprosum, and bacillary persistence. The Journal of Investigative Dermatology, 86(5), 529-34.
Levis WR, et al. IgM and IgG Antibodies to Phenolic Glycolipid I From Mycobacterium Leprae in Leprosy: Insight Into Patient Monitoring, Erythema Nodosum Leprosum, and Bacillary Persistence. J Invest Dermatol. 1986;86(5):529-34. PubMed PMID: 3528312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IgM and IgG antibodies to phenolic glycolipid I from Mycobacterium leprae in leprosy: insight into patient monitoring, erythema nodosum leprosum, and bacillary persistence. AU - Levis,W R, AU - Meeker,H C, AU - Schuller-Levis,G, AU - Sersen,E, AU - Schwerer,B, PY - 1986/5/1/pubmed PY - 1986/5/1/medline PY - 1986/5/1/entrez SP - 529 EP - 34 JF - The Journal of investigative dermatology JO - J Invest Dermatol VL - 86 IS - 5 N2 - Serum IgM and IgG antibodies against Mycobacterium leprae-derived phenolic glycolipid I (PG) were determined in leprosy patients, contacts, and controls by enzyme-linked immunosorbent assay (ELISA). Anti-PG IgM levels increased from the tuberculoid (TT) to the lepromatous (LL) pole of the disease spectrum. There was a positive linear correlation between anti-PG IgM and bacillary index (BI). Patients with erythema nodosum leprosum (ENL) had lower levels of serum anti-PG IgM than non-ENL patients of comparable BI, suggesting that anti-PG IgM is involved in the pathogenesis of ENL. Initial observations indicate that high anti-PG IgM levels in bacillary-negative patients might reflect bacillary persistence. A study of 2 different substrate reagents in the ELISA [2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 0.1 mM H2O2, serum diluted 1:20, and o-phenylenediamine (OPD), 5 mM H2O2, serum diluted 1:300] showed generally good correlation in detection of anti-PG IgM. However the OPD system detected more paucibacillary disease (BT), while the ABTS system detected the significant effect of ENL on the relationship between BI and anti-PG IgM. Anti-PG IgM was clearly dominant over anti-PG IgG. However, certain patients, including several patients who had upgraded from LL and borderline lepromatous leprosy (BL), showed high levels of anti-PG IgG. Since studies have shown that LL patients are selectively deficient in cell-mediated immunity, T-cell products may be required for the IgM to IgG isotype switch. We conclude that anti-PG IgM is useful for monitoring the bacillary load in individual patients and should prove useful for leprosy control strategies. SN - 0022-202X UR - https://www.unboundmedicine.com/medline/citation/3528312/IgM_and_IgG_antibodies_to_phenolic_glycolipid_I_from_Mycobacterium_leprae_in_leprosy:_insight_into_patient_monitoring_erythema_nodosum_leprosum_and_bacillary_persistence_ DB - PRIME DP - Unbound Medicine ER -