Simple and sensitive nitric oxide biosensor based on the electrocatalysis of horseradish peroxidase on AuNPs@metal-organic framework composite-modified electrode.
Mikrochim Acta. 2022 03 29; 189(4):162.MA

Abstract

Fe-based metal-organic framework (MIL-101(Fe)) was synthesized through a simple solvothermal synthesis and then used to prepare the AuNPs-decorated MIL-101(Fe) nanocomposite (APPPM(Fe)) by a multi-step layer-by-layer assembly process. Benefited from the porous structure of MIL-101(Fe) and the multilayer assemble process, the loading amount of AuNPs on APPPM(Fe) was enhanced and exhibited a fine biocompatible interface and high conductivity. Through the intense Au-S bond, high loading amount of horseradish peroxidase was immobilized on APPPM(Fe) and the native bioactivity of HRP was kept to realize its direct electrochemistry. From the electrochemical kinetics, the constructed biosensor displayed fast electron transfer and good electrocatalysis activity for the detection of nitric oxide (NO) with wide linear range from 0.033 to 5370 μM and a low detection limit of 0.01 μM (3 σ) as well as fine stability, reproducibility and specificity. According to results of real sample analysis, the proposed electrochemical biosensor offers fast and simple detection of NO in real serum. Therefore, the present strategy definitely provided a potential application prospect in NO clinic detection and disease therapy.

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Authors+Show Affiliations

Wang Y

Antibiotics Research and Re-Evaluation Key Laboratory of Sichuan Province, Chengdu University, Chengdu, 610106, China. Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Chengdu University, Chengdu, 610106, China.

Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Chengdu University, Chengdu, 610106, China. lihuiming@cdu.edu.cn. College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, 610106, China. lihuiming@cdu.edu.cn.

Liu K

Antibiotics Research and Re-Evaluation Key Laboratory of Sichuan Province, Chengdu University, Chengdu, 610106, China. liukunping@cdu.edu.cn. Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Chengdu University, Chengdu, 610106, China. liukunping@cdu.edu.cn.

MeSH

Biosensing TechniquesElectrodesGoldHorseradish PeroxidaseMetal NanoparticlesMetal-Organic FrameworksNitric OxideReproducibility of Results

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

35348908

Citation

Wang, Yong, et al. "Simple and Sensitive Nitric Oxide Biosensor Based On the Electrocatalysis of Horseradish Peroxidase On AuNPs@metal-organic Framework Composite-modified Electrode." Mikrochimica Acta, vol. 189, no. 4, 2022, p. 162.

Wang Y, Zhou Y, Chen Y, et al. Simple and sensitive nitric oxide biosensor based on the electrocatalysis of horseradish peroxidase on AuNPs@metal-organic framework composite-modified electrode. Mikrochim Acta. 2022;189(4):162.

Wang, Y., Zhou, Y., Chen, Y., Yin, Z., Hao, J., Li, H., & Liu, K. (2022). Simple and sensitive nitric oxide biosensor based on the electrocatalysis of horseradish peroxidase on AuNPs@metal-organic framework composite-modified electrode. Mikrochimica Acta, 189(4), 162. https://doi.org/10.1007/s00604-022-05268-8

Wang Y, et al. Simple and Sensitive Nitric Oxide Biosensor Based On the Electrocatalysis of Horseradish Peroxidase On AuNPs@metal-organic Framework Composite-modified Electrode. Mikrochim Acta. 2022 03 29;189(4):162. PubMed PMID: 35348908.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Simple and sensitive nitric oxide biosensor based on the electrocatalysis of horseradish peroxidase on AuNPs@metal-organic framework composite-modified electrode. AU - Wang,Yong, AU - Zhou,Youting, AU - Chen,Yangjie, AU - Yin,Zhihang, AU - Hao,Juan, AU - Li,Huiming, AU - Liu,Kunping, Y1 - 2022/03/29/ PY - 2021/12/19/received PY - 2022/03/08/accepted PY - 2022/3/29/entrez PY - 2022/3/30/pubmed PY - 2022/4/5/medline KW - Biosensor KW - Electrocatalysis KW - Horseradish peroxidase KW - Metal–organic framework KW - Nanocomposite KW - Nitric oxide SP - 162 EP - 162 JF - Mikrochimica acta JO - Mikrochim Acta VL - 189 IS - 4 N2 - Fe-based metal-organic framework (MIL-101(Fe)) was synthesized through a simple solvothermal synthesis and then used to prepare the AuNPs-decorated MIL-101(Fe) nanocomposite (APPPM(Fe)) by a multi-step layer-by-layer assembly process. Benefited from the porous structure of MIL-101(Fe) and the multilayer assemble process, the loading amount of AuNPs on APPPM(Fe) was enhanced and exhibited a fine biocompatible interface and high conductivity. Through the intense Au-S bond, high loading amount of horseradish peroxidase was immobilized on APPPM(Fe) and the native bioactivity of HRP was kept to realize its direct electrochemistry. From the electrochemical kinetics, the constructed biosensor displayed fast electron transfer and good electrocatalysis activity for the detection of nitric oxide (NO) with wide linear range from 0.033 to 5370 μM and a low detection limit of 0.01 μM (3 σ) as well as fine stability, reproducibility and specificity. According to results of real sample analysis, the proposed electrochemical biosensor offers fast and simple detection of NO in real serum. Therefore, the present strategy definitely provided a potential application prospect in NO clinic detection and disease therapy. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/35348908/Simple_and_sensitive_nitric_oxide_biosensor_based_on_the_electrocatalysis_of_horseradish_peroxidase_on_AuNPs@metal_organic_framework_composite_modified_electrode_ DB - PRIME DP - Unbound Medicine ER -

Tags

Simple and sensitive nitric oxide biosensor based on the electrocatalysis of horseradish peroxidase on AuNPs@metal-organic framework composite-modified electrode.Mikrochim Acta. 2022 03 29; 189(4):162.MA